Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Mouse Tissue sections (thyroid tissue)
Specification
thyroid tissue
Permeabilization
Yes - 0.1% Tween 20
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: 10% goat serum in PBS.
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: goat antirabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Dilution
1/500
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Sanjay Gawade
Verified customer
投稿 Jun 26 2013