Application
Western blot
Sample
Human Recombinant protein
Loading amount
0.25 µg
Blocking step
Milk as blocking agent for 11 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/5000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
1 second(s)
Additional data
Additional Notes
The antibody was excellent. Even when 0.5ug of peptide was loaded and membrane was exposed for a longer period, there was no cross reactivity. This is one of the best H3K27ac antibodies I have come across.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 28 2017