Application
ChIP
Sample
Human Cell lysate - nuclear (thyroid cancer)
Negative control
negative control: published negative region (10.1093/nar/gkx802)
Specification
thyroid cancer
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldheyde
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldheyde
Positive control
positive control promoter: GAPDH promoter
positive control enhancer: enhancer region of a published target (10.1093/nar/gkx802)
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Concentration
1 µg/µg chromatin
Additional data
Additional Notes
Worked well for Chip and Chip-Seq
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 01 2021