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Epigenetics and Nuclear Signaling Histones H3 Acetylated
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Anti-Histone H3 (acetyl K18) 抗体 - ChIP Grade (ab1191)

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Reviews (8)Q&A (1)References (147)

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ChIP - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (ab1191)
  • Peptide Array - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (ab1191)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (ab1191)
  • Western blot - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (ab1191)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (ab1191)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (ab1191)
  • Western blot - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (ab1191)

Key features and details

  • Rabbit polyclonal to Histone H3 (acetyl K18) - ChIP Grade
  • Suitable for: WB, PepArr, IHC-P, ICC/IF, ChIP
  • Reacts with: Mouse, Human, Arabidopsis thaliana
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Histone H3 (acetyl K18) antibody [EP959Y] - ChIP Grade (ab40888)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Histone H3 (acetyl K18) antibody - ChIP Grade
    Histone H3 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Histone H3 (acetyl K18) - ChIP Grade
  • 由来種

    Rabbit
  • 特異性

    Specificity for acetyl K18 has been validated by blocking peptide/antibody incubation followed by immunostaining western blots.
  • アプリケーション

    適用あり: WB, PepArr, IHC-P, ICC/IF, ChIPmore details
  • 種交差性

    交差種: Mouse, Human, Arabidopsis thaliana
    交差が予測される動物種: Rat, Cow, Saccharomyces cerevisiae, Drosophila melanogaster, Fungi
  • 免疫原

    Synthetic peptide corresponding to Histone H3 aa 1-100 (acetyl K18) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab24003)

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • Histones
    • H3
    • Acetylated
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies

関連製品

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Control Peptide

    • Human Histone H4 (acetyl K12) peptide (ab15662)
    • Human Histone H3 (acetyl K9) peptide (ab16635)
    • Human Histone H3 (acetyl K27) peptide (ab24404)
    • Human Histone H3 (acetyl K23) peptide (ab48359)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Histone H3 protein (Active) (ab198757)
  • Related Products

    • Histone H3 (acetyl K18) Quantification Kit (Colorimetric) (ab115108)
    • Histone Deacetylase (HDAC) Activity Assay Kit (Fluorometric) (ab156064)
    • Histone Acetyltransferase Activity Assay Kit (Colorimetric) (ab65352)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab1191の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (3)
1/1000. Detects a band of approximately 17 kDa.
PepArr
Use a concentration of 0.2 - 0.02 µg/ml.
IHC-P
1/200.
ICC/IF (1)
Use a concentration of 1 µg/ml.
ChIP (4)
Use 2µg for 106 cells.
特記事項
WB
1/1000. Detects a band of approximately 17 kDa.
PepArr
Use a concentration of 0.2 - 0.02 µg/ml.
IHC-P
1/200.
ICC/IF
Use a concentration of 1 µg/ml.
ChIP
Use 2µg for 106 cells.

ターゲット情報

  • 機能

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 配列類似性

    Belongs to the histone H3 family.
  • 発生段階

    Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
  • 翻訳後修飾

    Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
    Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
    Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
    Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
    Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
    Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
  • 細胞内局在

    Nucleus. Chromosome.
  • Target information above from: UniProt accession P68431 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 8350 Human
    • Entrez Gene: 8351 Human
    • Entrez Gene: 8352 Human
    • Entrez Gene: 8353 Human
    • Entrez Gene: 8354 Human
    • Entrez Gene: 8355 Human
    • Entrez Gene: 8356 Human
    • Entrez Gene: 8357 Human
    • Entrez Gene: 8358 Human
    • Entrez Gene: 8968 Human
    • Entrez Gene: 319152 Mouse
    • Entrez Gene: 319153 Mouse
    • Entrez Gene: 360198 Mouse
    • Entrez Gene: 97908 Mouse
    • Entrez Gene: 100364501 Rat
    • Entrez Gene: 100365669 Rat
    • Entrez Gene: 291159 Rat
    • Entrez Gene: 679994 Rat
    • Entrez Gene: 680511 Rat
    • Entrez Gene: 682330 Rat
    • Omim: 602810 Human
    • SwissProt: P68432 Cow
    • SwissProt: P68431 Human
    • SwissProt: P68433 Mouse
    • SwissProt: Q6LED0 Rat
    • Unigene: 132854 Human
    • Unigene: 247813 Human
    • Unigene: 247814 Human
    • Unigene: 248176 Human
    • Unigene: 443021 Human
    • Unigene: 484990 Human
    • Unigene: 532144 Human
    • Unigene: 533292 Human
    • Unigene: 546315 Human
    • Unigene: 586261 Human
    • Unigene: 591778 Human
    • Unigene: 221301 Mouse
    • Unigene: 261657 Mouse
    • Unigene: 377874 Mouse
    • Unigene: 390558 Mouse
    • Unigene: 397328 Mouse
    • Unigene: 138090 Rat
    see all
  • 別名

    • H3 histone family member E pseudogene antibody
    • H3 histone family, member A antibody
    • H3/A antibody
    • H31_HUMAN antibody
    • H3F3 antibody
    • H3FA antibody
    • Hist1h3a antibody
    • HIST1H3B antibody
    • HIST1H3C antibody
    • HIST1H3D antibody
    • HIST1H3E antibody
    • HIST1H3F antibody
    • HIST1H3G antibody
    • HIST1H3H antibody
    • HIST1H3I antibody
    • HIST1H3J antibody
    • HIST3H3 antibody
    • histone 1, H3a antibody
    • Histone cluster 1, H3a antibody
    • Histone H3 3 pseudogene antibody
    • Histone H3.1 antibody
    • Histone H3/a antibody
    • Histone H3/b antibody
    • Histone H3/c antibody
    • Histone H3/d antibody
    • Histone H3/f antibody
    • Histone H3/h antibody
    • Histone H3/i antibody
    • Histone H3/j antibody
    • Histone H3/k antibody
    • Histone H3/l antibody
    see all

画像

  • ChIP - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (ab1191)
    ChIP - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (ab1191)

    Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab1191 (blue), and 20 µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.

  • Peptide Array - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (ab1191)
    Peptide Array - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (ab1191)

    All batches of ab1191 are tested in Peptide Array against peptides to different Histone H3 and H4 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - acetyl K18 peptide (ab24003), indicating that this antibody specifically recognises the Histone H3 - acetyl K18 modification.

    ab17163 - Histone H3 - unmodified

    ab16635 - Histone H3 - acetyl K9

    ab15591 - Histone H3 - acetyl K14

    ab24003 - Histone H3 - acetyl K18

    ab48359 - Histone H3 - acetyl K23

    ab24404 - Histone H3 - acetyl K27

    ab41409 - Histone H3 - acetyl K36

    ab15662 - Histone H4 - acetyl K12

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (ab1191)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (ab1191)

    ab1191 stained in HeLa cells. Cells were fixed with 4% paraformaldehyde (10 min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% Triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab1191 at 0.1µg/ml and ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.

  • Western blot - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (ab1191)
    Western blot - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (ab1191)
    All lanes : Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (ab1191) at 1 µg/ml

    Lane 1 : HeLa Histone Preparation Nuclear Lysate
    Lane 2 : HeLa Histone Preparation Nuclear Lysate with Histone H3 peptide - unmodified R17 at 0.5 µg/ml
    Lane 3 : HeLa Histone Preparation Nuclear Lysate with Human Histone H3 (acetyl K9) peptide (ab16635) at 0.5 µg/ml
    Lane 4 : HeLa Histone Preparation Nuclear Lysate with Histone H3 peptide - acetyl K14 at 0.5 µg/ml
    Lane 5 : HeLa Histone Preparation Nuclear Lysate with Human Histone H3 (acetyl K18) peptide (ab24003) at 0.5 µg/ml
    Lane 6 : HeLa Histone Preparation Nuclear Lysate with Human Histone H3 (acetyl K23) peptide (ab48359) at 0.5 µg/ml
    Lane 7 : HeLa Histone Preparation Nuclear Lysate with Human Histone H3 (acetyl K27) peptide (ab24404) at 0.5 µg/ml
    Lane 8 : HeLa Histone Preparation Nuclear Lysate with Histone H3 peptide - acetyl K36 at 0.5 µg/ml
    Lane 9 : HeLa Histone Preparation Nuclear Lysate with Human Histone H4 (acetyl K12) peptide (ab15662) at 0.5 µg/ml

    Lysates/proteins at 2.5 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 15.4 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (ab1191)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (ab1191)This image is courtesy of Danièle Roche, Geneviève Almouzni's lab, UMR218
    L929, 3T3 and HeLa cells fixed with 2% paraformaldehyde in PBS were incubated for 5 min in 0.2% Triton X-100 in PBS before a blocking step in 5% BSA, 0.1% Tween 20 in PBS (blocking buffer). Ac K18-H3 (ab1191) antibody was added diluted at 1/500 in blocking buffer for a 45 min incubation at room temperature. For visualization, fluorescein isothiocyanate (FITC) anti rabbit secondary antibody was used diluted at 1/800. DAPI (Sigma) in 0.1% Tween 20 in PBS was used in the final washing steps to stain DNA. Vectashield (Vector Laboratories Inc.) was used as mounting medium.
    The ac K18-H3 antibody revealed a granular staining throughout the nucleoplasm, excluding regions that show an intense DAPI staining (mostly pericentric heterochromatin in mice cells).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (ab1191)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (ab1191)

    IHC image of Histone H3 (acetyl K18) staining in Human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1191, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (ab1191)
    Western blot - Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (ab1191)
    All lanes : Anti-Histone H3 (acetyl K18) antibody - ChIP Grade (ab1191) at 1 µg/ml

    Lane 1 : Histone prep
    Lane 2 : Histone prep with Human Histone H3 (acetyl K18) peptide (ab24003) at 1 µg/ml

    Lysates/proteins at 0.5 µg/ml per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

    Predicted band size: 15.4 kDa

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (147)

ab1191 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab1191 は 147 報の論文で使用されています。

  • Chen L  et al. The failure of DAC to induce OCT2 expression and its remission by hemoglobin-based nanocarriers under hypoxia in renal cell carcinoma. Theranostics 10:3562-3578 (2020). PubMed: 32206108
  • Hirano K  et al. Indonesian Ginger (Bangle) Extract Promotes Neurogenesis of Human Neural Stem Cells through WNT Pathway Activation. Int J Mol Sci 21:N/A (2020). PubMed: 32635647
  • Martire S  et al. Differential contribution of p300 and CBP to regulatory element acetylation in mESCs. BMC Mol Cell Biol 21:55 (2020). PubMed: 32690000
  • Bi S  et al. SIRT7 antagonizes human stem cell aging as a heterochromatin stabilizer. Protein Cell 11:483-504 (2020). PubMed: 32504224
  • Ebrahimi A  et al. Bromodomain inhibition of the coactivators CBP/EP300 facilitate cellular reprogramming. Nat Chem Biol 15:519-528 (2019). PubMed: 30962627
View all Publications for this product

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1-9 of 9 Abreviews or Q&A

Western blot abreview for Anti-Histone H3 (acetyl K18) antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (15%)
Sample
Human Cell lysate - whole cell (Huvec)
Specification
Huvec
Treatment
MS275
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 4°C
Read More

Abcam user community

Verified customer

投稿 Nov 05 2013

Western blot

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (12% SDS gel)
Sample
Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Whole mosquito (Culex pipiens))
Specification
Whole mosquito (Culex pipiens)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 May 30 2013

Western blot abreview for Anti-Histone H3 (acetyl K18) antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (s. cerevisiae cells)
Loading amount
10 µg
Specification
s. cerevisiae cells
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

投稿 Aug 16 2011

ChIP abreview for Anti-Histone H3 (acetyl K18) antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
ChIP
Sample
Human Cell lysate - nuclear (5 million LNCaP cells/IP)
Specification
5 million LNCaP cells/IP
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step
Real-time PCR
Positive control
PSA enhancer
Negative control
rabbit IgG
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Abcam user community

Verified customer

投稿 Jul 01 2010

ChIP abreview for Anti-Histone H3 (acetyl K18) antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
ChIP
Sample
Human Cell lysate - whole cell (HeLa)
Specification
HeLa
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step
Other
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Lindsey Harp

Verified customer

投稿 Nov 30 2006

Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (acetyl K18) antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Arabidopsis thaliana Cell (Culture cell, ecotype Lansberg)
Specification
Culture cell, ecotype Lansberg
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3%
Read More

Dr. Peter McKeown

Verified customer

投稿 Aug 04 2006

Question

what I should use for a positive control? We use this antibodies for parafine section of breast cancers ab1191 - h3 (acetyl K18) ab1761 - h4 (acetyl K12) ab4441 - h3 (acetyl K9) ab7766 - h3 (dimethyl K4)

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Abcam community

Verified customer

Asked on Sep 20 2005

Answer

These histone modifications are ubiquitous across all cell types (except yeast which do not have acetylation at lysine 12 of H4). You could use a standard HeLa cell line and prepare them as detailed in the abcam immunocytochemistry protocol (http://ops.abcam.com/index.html?pageconfig=view_protocol&pid=159) or alternatively, http://ops.abcam.com/index.html?pageconfig=protocols&pid=174&intAbID=7766&strTab=protocols&mode=prot It is worth noting that you can elevate the level of histone acetylation across all of the lysine residues you have antibodies against by incubating (if possible) with Na Butyrate (5mM). This will in turn improve your levels of staining.

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Abcam Scientific Support

Answered on Sep 21 2005

ChIP abreview for Anti-Histone H3 (acetyl K18) antibody - ChIP Grade

Poor
Abreviews
Abreviews
abreview image
Application
ChIP
Sample
Saccharomyces cerevisiae Cell lysate - whole cell
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Detection step
Semiquantitative PCR
Positive control
The ChIP positive control used in this experiment was an rpd3 knock out Saccharomyces cerevisiae strain refered to as wt because it contains no amino acid substitutions at the H3 amino tail. Following ChIP the positive control locus INO1 was amplified by PCR. INO1 acetylation levels have been shown previously to be regulated by Rpd3 and are elevated for all sites of acetylation on histone H3.
Negative control
Negative control loci SPS2 and TEL were amplified concurrently with INO1. Both loci have been shown to have depleted levels of H3 acetylation and to be independently regulated of Rpd3.
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Maria Vogelauer

Verified customer

投稿 Sep 16 2005

ChIP abreview for Anti-Histone H3 (acetyl K18) antibody - ChIP Grade

Good
Abreviews
Abreviews
Application
ChIP
Sample
Mouse Cell lysate - nuclear (Macrophages)
Specification
Macrophages
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: formaldehyde
Detection step
Real-time PCR
Positive control
Rabbit IgGs
Read More

Abcam user community

Verified customer

投稿 Jul 29 2005

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