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    histone-h2b-antibody-chip-grade-ab1790.pdf

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Epigenetics and Nuclear Signaling ChIP assays ChIP antibodies
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Anti-Histone H2B 抗体 - ChIP Grade (ab1790)

  • Datasheet
Reviews (28)Q&A (20)References (204)

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Western blot - Anti-Histone H2B antibody - ChIP Grade (ab1790)
  • ChIP - Anti-Histone H2B antibody - ChIP Grade (ab1790)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B antibody - ChIP Grade (ab1790)
  • Immunoprecipitation - Anti-Histone H2B antibody - ChIP Grade (ab1790)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B antibody - ChIP Grade (ab1790)
  • ChIP - Anti-Histone H2B antibody - ChIP Grade (ab1790)
  • Western blot - Anti-Histone H2B antibody - ChIP Grade (ab1790)
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B antibody - ChIP Grade (ab1790)
  • Western blot - Anti-Histone H2B antibody - ChIP Grade (ab1790)

Key features and details

  • Rabbit polyclonal to Histone H2B - ChIP Grade
  • Suitable for: WB, IHC-P, ChIP, ICC/IF, IP
  • Reacts with: Cow, Human, Saccharomyces cerevisiae, Xenopus laevis, Arabidopsis thaliana
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Histone H2B antibody [EP957Y] (ab52599)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Histone H2B antibody - ChIP Grade
    Histone H2B 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Histone H2B - ChIP Grade
  • 由来種

    Rabbit
  • 特異性

    This antibody is specific for Histone 2B.
  • アプリケーション

    適用あり: WB, IHC-P, ChIP, ICC/IF, IPmore details
  • 種交差性

    交差種: Cow, Human, Saccharomyces cerevisiae, Xenopus laevis, Arabidopsis thaliana
    交差が予測される動物種: Mouse, Rat, Chicken, Zebrafish
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab16101)

  • ポジティブ・コントロール

    • Calf Thymus Histone Preparation This antibody gave a positive result when used in the following methanol fixed cell lines: HeLa
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 軽鎖の種類

    unknown
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies

関連製品

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human Histone H2B peptide (ab16101)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Histone H2B protein (ab92409)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab1790の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (18)
Use a concentration of 0.1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa).Can be blocked with Human Histone H2B peptide (ab16101).
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ChIP (3)
Use a concentration of 2 - 3 µg/ml.
ICC/IF (2)
Use a concentration of 0.5 µg/ml.
IP (3)
Use a concentration of 5 µg/ml.
特記事項
WB
Use a concentration of 0.1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa).Can be blocked with Human Histone H2B peptide (ab16101).
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ChIP
Use a concentration of 2 - 3 µg/ml.
ICC/IF
Use a concentration of 0.5 µg/ml.
IP
Use a concentration of 5 µg/ml.

ターゲット情報

  • 関連性

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Post-translational modification Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription. Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
  • 細胞内局在

    Nuclear
  • 参照データベース

    • Entrez Gene: 337874 Human
    • Entrez Gene: 54145 Human
    • Entrez Gene: 8349 Human
    • Entrez Gene: 104021 Mouse
    • Entrez Gene: 64647 Rat
    • SwissProt: P0C1H5 Chicken
    • SwissProt: P62808 Cow
    • SwissProt: O60814 Human
    • SwissProt: P06899 Human
    • SwissProt: P23527 Human
    • SwissProt: P33778 Human
    • SwissProt: P57053 Human
    • SwissProt: P58876 Human
    • SwissProt: P62807 Human
    • SwissProt: Q16778 Human
    • SwissProt: Q5QNW6 Human
    • SwissProt: Q8N257 Human
    • SwissProt: Q93079 Human
    • SwissProt: Q99877 Human
    • SwissProt: Q99879 Human
    • SwissProt: Q99880 Human
    • SwissProt: P10854 Mouse
    • SwissProt: Q64475 Mouse
    • SwissProt: Q00729 Rat
    see all
  • 別名

    • GL105 antibody
    • H2B GL105 antibody
    • H2B histone family member O antibody
    • H2B histone family member S antibody
    • H2B.1 antibody
    • H2B.1 B antibody
    • H2B.b antibody
    • H2B.c antibody
    • H2B.d antibody
    • H2B.e antibody
    • H2B.f antibody
    • H2B.j antibody
    • H2B.q antibody
    • H2B/b antibody
    • H2B/c antibody
    • H2B/d antibody
    • H2B/e antibody
    • H2B/f antibody
    • H2B/j antibody
    • H2B/o antibody
    • H2B/q antibody
    • H2BFB antibody
    • H2BFC antibody
    • H2BFD antibody
    • H2BFE antibody
    • H2BFF antibody
    • H2BFJ antibody
    • H2BFO antibody
    • H2BFQ antibody
    • H2BFS antibody
    • H2BGL105 antibody
    • H2BQ antibody
    • HIRIP2 antibody
    • HIST1H2BB antibody
    • HIST1H2BD antibody
    • HIST1H2BH antibody
    • HIST1H2BL antibody
    • HIST1H2BM antibody
    • HIST1H2BN antibody
    • HIST2H2BE antibody
    • histone cluster 2 antibody
    • Histone H2B antibody
    • histone H2B GL105 antibody
    • Histone H2B type 1 B antibody
    • Histone H2B type 1 D antibody
    • Histone H2B type 1 H antibody
    • Histone H2B type 1 L antibody
    • Histone H2B type 1 M antibody
    • Histone H2B type 1 N antibody
    • Histone H2B type 2 E antibody
    • Histone H2B.q antibody
    • histone protein antibody
    see all

画像

  • Western blot - Anti-Histone H2B antibody - ChIP Grade (ab1790)
    Western blot - Anti-Histone H2B antibody - ChIP Grade (ab1790)
    All lanes : Anti-Histone H2B antibody - ChIP Grade (ab1790)

    Lane 1 : Hela Histone prep
    Lane 2 : Hela whole cell lysate
    Lane 3 : S. cerevisiae whole cell lysate

    Performed under reducing conditions.

    Predicted band size: 14 kDa

  • ChIP - Anti-Histone H2B antibody - ChIP Grade (ab1790)
    ChIP - Anti-Histone H2B antibody - ChIP Grade (ab1790)

    Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 2µg of ab1790 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B antibody - ChIP Grade (ab1790)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B antibody - ChIP Grade (ab1790)This image is courtesy of Roberto Giambruno and Patrizia Lavia

    HeLa cells were fixed in 100% methanol for 6 minutes at -20°C. The cells were washed 3 times in PBS then incubated with ab1790 (0.5µg/ml) for 1 hour at room temperature. The panel of images shows the cells stained with ab1790 (green) and counterstained with DAPI (blue). 100x magnification.

  • Immunoprecipitation - Anti-Histone H2B antibody - ChIP Grade (ab1790)
    Immunoprecipitation - Anti-Histone H2B antibody - ChIP Grade (ab1790)

    Histone H2B - ChIP Grade was immunoprecipitated using 0.5mg HeLa whole cell extract, 5µg of Rabbit polyclonal to and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, HeLa whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab1790.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 14kDa; Histone H2B - ChIP Grade

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B antibody - ChIP Grade (ab1790)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2B antibody - ChIP Grade (ab1790)
    IHC image of Histone H2B staining in human breast carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1790, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • ChIP - Anti-Histone H2B antibody - ChIP Grade (ab1790)
    ChIP - Anti-Histone H2B antibody - ChIP Grade (ab1790)

    Chromatin from Xenopus laevis oocytes was prepared according to the Abcam X-ChIP protocol. Oocytes were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 3 µg of ab1790 (anti-H2B, light blue) and 3 µg of ab1791 (anti-H3, dark blue), and 20 µl of Protein A/G sepharose beads. A non-specific antibody was used as a control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach).

  • Western blot - Anti-Histone H2B antibody - ChIP Grade (ab1790)
    Western blot - Anti-Histone H2B antibody - ChIP Grade (ab1790)
    All lanes : Anti-Histone H2B antibody - ChIP Grade (ab1790) at 0.1 µg/ml

    Lane 1 : Calf thymus histone prep
    Lane 2 : Calf thymus histone prep with Human Histone H2B peptide (ab16101) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Alexa fluor Goat polyclonal anti-Rabbit IgG at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 14 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B antibody - ChIP Grade (ab1790)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B antibody - ChIP Grade (ab1790)

    ICC/IF image of ab1790 stained HeLa cells. The cells were 100% methanol fixed (5 min) then permeabilised using 0.1% PBS-Triton and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to further permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab1790 at 0.1µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Western blot - Anti-Histone H2B antibody - ChIP Grade (ab1790)
    Western blot - Anti-Histone H2B antibody - ChIP Grade (ab1790)This image is courtesy of an Abreview submitted by Dr Igor Landais
    ab1790 at 1/3000 detecting Histone H2B from Xenopus laevis (S phase egg extracts - whole cell lysates 60ug per lane) by Western Blot. The egg extracts were fractionated using a gel filtration column and every other fraction (4 - 26) was loaded onto a 8-16% gel. The input corresponds to 1ul of crude extract. In this experiment an HRP conjugated donkey anti-rabbit antibody was used as the secondary.

    See Abreview

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

データシートおよび資料

  • Datasheet download

    Download

参考文献 (204)

ab1790 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab1790 は 204 報の論文で使用されています。

  • Zhang L  et al. Function of histone H2B monoubiquitination in transcriptional regulation of auxin biosynthesis in Arabidopsis. Commun Biol 4:206 (2021). PubMed: 33589721
  • Itoh Y  et al. 1,6-hexanediol rapidly immobilizes and condenses chromatin in living human cells. Life Sci Alliance 4:N/A (2021). PubMed: 33536240
  • Suraweera A  et al. COMMD4 functions with the histone H2A-H2B dimer for the timely repair of DNA double-strand breaks. Commun Biol 4:484 (2021). PubMed: 33875784
  • Norin U  et al. Endophilin A2 deficiency protects rodents from autoimmune arthritis by modulating T cell activation. Nat Commun 12:610 (2021). PubMed: 33504785
  • Silva Cascales H  et al. Cyclin A2 localises in the cytoplasm at the S/G2 transition to activate PLK1. Life Sci Alliance 4:N/A (2021). PubMed: 33402344
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 48 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H2B antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cells)
Specification
HeLa cells
Fixative
Formaldehyde
Permeabilization
Yes - 0.1 % TritonX-100 for 5 minutes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Jul 13 2012

Immunoprecipitation abreview for Anti-Histone H2B antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - nuclear (HeLa cell line)
Total protein in input
5e+006 cells
Specification
HeLa cell line
Immuno-precipitation step
Protein A
Read More

Abcam user community

Verified customer

投稿 Mar 28 2011

Blocking abreview for Anti-Histone H2B antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Blocking
Sample
Human Cell lysate - nuclear (gastric)
Specification
gastric
Read More

Abcam user community

Verified customer

投稿 Apr 19 2019

Western blot abreview for Anti-Histone H2B antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (spinal cord)
Gel Running Conditions
Reduced Denaturing (Bis Tris 4-12%)
Loading amount
25 µg
Specification
spinal cord
Blocking step
(agent) for 30 minute(s) · Concentration: 0.001% · Temperature: 25°C
Read More

DR. Gillian Hunter

Verified customer

投稿 Feb 15 2019

Western blot abreview for Anti-Histone H2B antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - nuclear (MEF)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
MEF
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Apr 06 2017

Western blot abreview for Anti-Histone H2B antibody - ChIP Grade

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
HeLa
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Jul 01 2016

Immunohistochemistry (Resin sections) abreview for Anti-Histone H2B antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Resin sections)
Sample
Astatotilapia burtoni Tissue sections (Brain Preoptic area LRWhite embedded)
Specification
Brain Preoptic area LRWhite embedded
Read More

Sebastian Alvarado

Verified customer

投稿 May 03 2016

Western blot abreview for Anti-Histone H2B antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
0.5 µg
Gel Running Conditions
Reduced Denaturing (18% gel)
Sample
Human Recombinant protein (Recombinant octamers & purified K-562 cell histone)
Specification
Recombinant octamers & purified K-562 cell histone
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

DR. Ragnhild Eskeland

Verified customer

投稿 Mar 04 2015

Western blot abreview for Anti-Histone H2B antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Xenopus laevis Cell lysate - nuclear (Egg extract (total and chromatin))
Specification
Egg extract (total and chromatin)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

投稿 Sep 03 2013

Question

I will test your new lot of antibody... hoping that it works as well as the old one.
The order number is GGP10032A/0038
Best regard

Read More

Abcam community

Verified customer

Asked on Oct 29 2012

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1192133.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Abcam Scientific Support

Answered on Oct 29 2012

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