Anti-Histone H2B (acetyl K20) 抗体 [RM235] (ab271344)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM235] to Histone H2B (acetyl K20)
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Human
製品の概要
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製品名
Anti-Histone H2B (acetyl K20) antibody [RM235]
Histone H2B 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [RM235] to Histone H2B (acetyl K20) -
由来種
Rabbit -
特異性
No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.
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アプリケーション
適用あり: WB, ELISA, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Histone H2B (acetyl K20).
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ポジティブ・コントロール
- ICC/IF: HeLa cells treated with sodium butyrate. WB: HeLa cells treated with sodium butyrate.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
RM235 -
アイソタイプ
IgG
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab271344の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 14 kDa.
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ELISA |
Use a concentration of 0.2 - 1 µg/ml.
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ICC/IF |
Use a concentration of 1 - 2 µg/ml.
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特記事項 |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 14 kDa. |
ELISA
Use a concentration of 0.2 - 1 µg/ml. |
ICC/IF
Use a concentration of 1 - 2 µg/ml. |
ターゲット情報
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関連性
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Post-translational modification Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons. Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription. Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 337874 Human
- Entrez Gene: 54145 Human
- Entrez Gene: 8349 Human
- SwissProt: O60814 Human
- SwissProt: P06899 Human
- SwissProt: P23527 Human
- SwissProt: P33778 Human
- SwissProt: P57053 Human
see all -
別名
- GL105 antibody
- H2B GL105 antibody
- H2B histone family member O antibody
see all
画像
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All lanes : Anti-Histone H2B (acetyl K20) antibody [RM235] (ab271344) at 0.5 µg/ml
Lane 1 : Acid extracts of HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with sodium butyrate
Lane 2 : Acid extracts of untreated HeLa cells
Predicted band size: 14 kDa -
Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells treated with sodium butyrate stained for Histone H2B (acetyl K20) using ab271344 at 2 µg/ml (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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ab271344 specifically reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab271344 は論文での使用が確認できていません。