Anti-Histone H2A.Z 抗体 [EPR6172] - ChIP Grade - BSA and Azide free (ab248007)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6172] to Histone H2A.Z - ChIP Grade – BSA and Azide free
- Suitable for: WB, IHC-P, ChIP-sequencing
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Histone H2A.Z antibody [EPR6172] - ChIP Grade - BSA and Azide free
Histone H2A.Z 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6172] to Histone H2A.Z - ChIP Grade – BSA and Azide free -
由来種
Rabbit -
特異性
This antibody is known to detect both isoforms of H2A.Z (H2A.Z.1 and H2A.Z.2).
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アプリケーション
適用あり: WB, IHC-P, ChIP-sequencingmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: C6, Raw 264.7, PC-12, NIH/3T3, HeLa, HepG2, and Human thymus lysates; IHC-P: Human breast tissue; ChIP-seq: Chromatin from HeLa cells.
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特記事項
ab248007 is the carrier-free version of ab124793.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6172 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248007の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended.
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ChIP-sequencing |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended.
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ChIP-sequencing
Use at an assay dependent concentration. |
ターゲット情報
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機能
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division. -
配列類似性
Belongs to the histone H2A family. -
翻訳後修飾
Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
Not phosphorylated. -
細胞内局在
Nucleus. Chromosome. - Information by UniProt
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参照データベース
- Entrez Gene: 3015 Human
- Entrez Gene: 51788 Mouse
- Entrez Gene: 58940 Rat
- Omim: 142763 Human
- SwissProt: P0C0S5 Human
- SwissProt: P0C0S6 Mouse
- SwissProt: P0C0S7 Rat
- Unigene: 119192 Human
see all -
別名
- H2A histone family member Z antibody
- H2A.z antibody
- H2A/z antibody
see all
画像
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All lanes : Anti-Histone H2A.Z antibody [EPR6172] - ChIP Grade (ab124793) at 1/1000 dilution
Lane 1 : C6 lysates
Lane 2 : Raw 264.7 lysates
Lane 3 : PC-12 lysates
Lane 4 : NIH/3T3 lysates
Lane 5 : HeLa lysates
Lane 6 : HepG2 lysates
Lane 7 : Human thymus lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 14 kDaThis data was developed using ab124793, the same antibody clone in a different buffer formulation.
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This data was developed using ab124793, the same antibody clone in a different buffer formulation.ab124793, at a 1/250 dilution, staining Histone H2A.Z in paraffin embedded Human breast tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab248007 は論文での使用が確認できていません。