Anti-Histone H2A.Z 抗体 [EPR6172] - ChIP Grade (ab124793)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6172] to Histone H2A.Z - ChIP Grade
- Suitable for: ChIP-sequencing, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Histone H2A.Z antibody [EPR6172] - ChIP Grade
Histone H2A.Z 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6172] to Histone H2A.Z - ChIP Grade -
由来種
Rabbit -
特異性
This antibody is known to detect both isoforms of H2A.Z (H2A.Z.1 and H2A.Z.2). -
アプリケーション
適用あり: ChIP-sequencing, WB, IHC-Pmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: C6, Raw 264.7, PC-12, NIH/3T3, HeLa, HepG2, and Human thymus lysates; IHC-P: Human breast tissue; ChIP-seq: Chromatin from HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6172 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124793の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ChIP-sequencing |
Use 4µg for 107 cells.
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WB | (5) |
1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
antigen retrieval is recommended. |
特記事項 |
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ChIP-sequencing
Use 4µg for 107 cells. |
WB
1/1000 - 1/10000. Predicted molecular weight: 14 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. antigen retrieval is recommended. |
ターゲット情報
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機能
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division. -
配列類似性
Belongs to the histone H2A family. -
翻訳後修飾
Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
Not phosphorylated. -
細胞内局在
Nucleus. Chromosome. - Information by UniProt
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参照データベース
- Entrez Gene: 3015 Human
- Entrez Gene: 51788 Mouse
- Entrez Gene: 58940 Rat
- Omim: 142763 Human
- SwissProt: P0C0S5 Human
- SwissProt: P0C0S6 Mouse
- SwissProt: P0C0S7 Rat
- Unigene: 119192 Human
see all -
別名
- H2A histone family member Z antibody
- H2A.z antibody
- H2A/z antibody
see all
画像
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Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 HeLa cells and 4 µg of ab124793 [EPR6172]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.
Additional screenshots of mapped reads can be downloaded here.
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All lanes : Anti-Histone H2A.Z antibody [EPR6172] - ChIP Grade (ab124793) at 1/1000 dilution
Lane 1 : C6 lysates
Lane 2 : Raw 264.7 lysates
Lane 3 : PC-12 lysates
Lane 4 : NIH/3T3 lysates
Lane 5 : HeLa lysates
Lane 6 : HepG2 lysates
Lane 7 : Human thymus lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 14 kDa -
ab124793, at a 1/250 dilution, staining Histone H2A.Z in paraffin embedded Human breast tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab124793 は論文での使用が確認できていません。