Anti-Histone H2A (phospho S129) 抗体 (ab17353)
Key features and details
- Rabbit polyclonal to Histone H2A (phospho S129)
- Suitable for: WB
- Reacts with: Schizosaccharomyces pombe
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Histone H2A (phospho S129) antibody
Histone H2A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Histone H2A (phospho S129) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Schizosaccharomyces pombe
交差が予測される動物種: Saccharomyces cerevisiae
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免疫原
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ポジティブ･コントロール
- S. pombe lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab17353の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 15.5 kDa (predicted molecular weight: 15.5 kDa).
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| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 15.5 kDa (predicted molecular weight: 15.5 kDa). |
ターゲット情報
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機能
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
配列類似性
Belongs to the histone H2A family. -
翻訳後修飾
The chromatin-associated form is phosphorylated on Thr-121 during mitosis.
Deiminated on Arg-4 in granulocytes upon calcium entry.
Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.
Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage. -
細胞内局在
Nucleus. Chromosome. - Information by UniProt
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参照データベース
- SwissProt: P04912 Saccharomyces cerevisiae
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別名
- H2a 615 antibody
- H2A antibody
- H2A GL101 antibody
see all
画像
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Western blot - Anti-Histone H2A (phospho S129) antibody (ab17353)ab17353 recognised a band of the predicted size in S. pombe lysate (lane 1). This band was blocked by the immunising peptide (lane 2) but not by the unmodified version of the immunising peptide (lane 3). This suggests specificity for the modified residue.
データシートおよび資料
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Datasheet download
参考文献 (9)
ab17353 は 9 報の論文で使用されています。
- Schmücker A et al. Crosstalk between H2A variant-specific modifications impacts vital cell functions. PLoS Genet 17:e1009601 (2021). PubMed: 34086674
- Tatebe H et al. Rad50 zinc hook functions as a constitutive dimerization module interchangeable with SMC hinge. Nat Commun 11:370 (2020). PubMed: 31953386
- Cal M et al. The Anticancer Drug 3-Bromopyruvate Induces DNA Damage Potentially Through Reactive Oxygen Species in Yeast and in Human Cancer Cells. Cells 9:N/A (2020). PubMed: 32397119
- Kim SM & Forsburg SL Active Replication Checkpoint Drives Genome Instability in Fission Yeast mcm4 Mutant. Mol Cell Biol 40:N/A (2020). PubMed: 32341083
- Jablonowski CM et al. Termination of Replication Stress Signaling via Concerted Action of the Slx4 Scaffold and the PP4 Phosphatase. Genetics 201:937-49 (2015). PubMed: 26362319
- Tapia-Alveal C et al. H2A.Z-dependent regulation of cohesin dynamics on chromosome arms. Mol Cell Biol 34:2092-104 (2014). PubMed: 24687850
- Lee M et al. Rad52/Rad59-dependent recombination as a means to rectify faulty Okazaki fragment processing. J Biol Chem 289:15064-79 (2014). PubMed: 24711454
- Garcia V et al. Bidirectional resection of DNA double-strand breaks by Mre11 and Exo1. Nature 479:241-4 (2011). PubMed: 22002605
- Outwin EA et al. Smc5-Smc6-dependent removal of cohesin from mitotic chromosomes. Mol Cell Biol 29:4363-75 (2009). WB ; Schizosaccharomyces pombe . PubMed: 19528228
