Anti-Histone H2A-Bbd 抗体 (ab4175)
Key features and details
- Rabbit polyclonal to Histone H2A-Bbd
- Suitable for: ICC, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Histone H2A-Bbd antibody -
製品の詳細
Rabbit polyclonal to Histone H2A-Bbd -
由来種
Rabbit -
特異性
Known to detect the recombinant form of human Histone H2A BBD. However, no reaction has yet been demonstrated against the endogenous protein in histone preparations. We believe that this is because of the low abundance of this variant in such preparations. -
アプリケーション
適用あり: ICC, WBmore details -
種交差性
交差種: Human -
免疫原
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab4175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC | Use a concentration of 3 µg/ml. Staining may not be specific for H2A.Bbd (see image). | |
| WB | 1/500. Detects a band of approximately 12.5 kDa (predicted molecular weight: 12.5 kDa). As the WB shows, we can not find any reaction with any of the different histone extracts that we have used from different sources (calf, human, chicken). However, the antibody is very specific against the recombinant form of the protein. |
ターゲット情報
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機能
Atypical histone H2A which can replace conventional H2A in some nucleosomes and is associated with active transcription and mRNA processing. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Nucleosomes containing this histone are less rigid and organize less DNA than canonical nucleosomes in vivo. They are enriched in actively transcribed genes and associate with the elongating form of RNA polymerase. They associate with spliceosome components and are required for mRNA splicing. May participate in spermatogenesis. -
組織特異性
Present in mature sperm. -
配列類似性
Belongs to the histone H2A family. -
ドメイン
The docking domain is responsible for the weaker heterodimerization with H2B. -
細胞内局在
Nucleus. Chromosome. Associated with the active X chromosome and with autosomes, while it is absent from the inactive X chromosome and excluded from Barr bodies. - Information by UniProt
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参照データベース
- Entrez Gene: 474381 Human
- Entrez Gene: 474382 Human
- Entrez Gene: 83740 Human
- Omim: 300445 Human
- SwissProt: P0C5Y9 Human
- SwissProt: P0C5Z0 Human
- Unigene: 534498 Human
- Unigene: 632841 Human
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別名
- H2A Barr body deficient antibody
- H2A Barr body-deficient antibody
- H2A histone family member B antibody
see all
画像
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Western blot - Anti-Histone H2A-Bbd antibody (ab4175)Juan AusioH2A-Bbd (ab4175)
CE= chicken erythrocyte (10 and 2 micrograms) , HE= HeLa cells (10 micrograms) and 2A*= recombinant H2ABbd (the numbers underneath the lanes are the number of nanograms loaded in each lane).
The primary antibody dilution was 1:500 and the secondary was 1:3000.As the WB shows, we can not find any reaction with any of the different histone extracts that we have used from different sources (calf, human, chicken). However, the antibody is very specific against the recombinant form of the protein.
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Immunocytochemistry - Anti-Histone H2A-Bbd antibody (ab4175)This image is courtesy of Petra Hajkova, University of CambridgeMouse embryonic fibroblasts (MEFs) were fixed in 4%PFA, permeabilised in PBS 1%BSA 0.1%TX-100 and incubated with ab4175 (anti-H2A.Bbd) antibody o/n. The antibody appears to show nucleolar staining. The antibody gave the same staining pattern in different cell types. The dilution used was 3ug/ml.
プロトコール
データシートおよび資料
参考文献 (3)
ab4175 は 3 報の論文で使用されています。
- Wu BJ et al. Localization and expression of histone H2A variants during mouse oogenesis and preimplantation embryo development. Genet Mol Res 13:5929-39 (2014). PubMed: 25117351
- Nashun B et al. Changes in the nuclear deposition of histone H2A variants during pre-implantation development in mice. Development 137:3785-94 (2010). WB, ICC/IF ; Mouse . PubMed: 20943707
- Lee JH et al. Chromatin analysis of occluded genes. Hum Mol Genet 18:2567-74 (2009). ChIP ; Human . PubMed: 19380460
