Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris)
Loading amount
25 µg
Specification
Brain
Blocking step
(agent) for 30 minute(s) · Concentration: 0.001% · Temperature: 25°C
Other product details
Incubation time
10 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odyssey block + 1:1000 Tween 20
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Goat anti rabbit 680
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye® 680RD
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye® 680RD
Detection
Detection method
Licor
Exposure
30 second(s)
Bands
Specific: 17, 25 kDa
Additional data
Additional Notes
Bottom band H2A, top band ubiquitinated H2A
All samples are whole protein lysates from mouse brain
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Gillian Hunter
Verified customer
投稿 Feb 15 2019