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  1. Link

    histone-h2a-acetyl-k5-antibody-ab1764.pdf

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Epigenetics and Nuclear Signaling Histones H2A Acetylated
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Anti-Histone H2A (acetyl K5) 抗体 (ab1764)

  • Datasheet
Reviews (10)Q&A (4)References (12)

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Abpromise

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody - ChIP Grade (ab1764)

    Key features and details

    • Rabbit polyclonal to Histone H2A (acetyl K5)
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-Histone H2A (acetyl K5) antibody
      Histone H2A 一次抗体 製品一覧
    • 製品の詳細

      Rabbit polyclonal to Histone H2A (acetyl K5)
    • 由来種

      Rabbit
    • アプリケーション

      適用あり: IHC-Pmore details
    • 種交差性

      交差種: Human
    • 免疫原

      Synthetic peptide corresponding to Histone H2A (acetyl K5) conjugated to ovalbumin.

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 特記事項

      ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • バッファー

      Preservative: 0.02% Sodium azide
    • Concentration information loading...
    • 精製度

      Whole antiserum
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      IgG
    • 研究分野

      • Epigenetics and Nuclear Signaling
      • Histones
      • H2A
      • Acetylated

    関連製品

    • ChIP Related Products

      • Rabbit Anti-Mouse IgG H&L (ab46540)
      • ChIP Kit (ab500)
    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Histone H2A (biotinylated ) protein (ab200286)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab1764の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P (2)
    1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    特記事項
    IHC-P
    1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    ターゲット情報

    • 機能

      Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
    • 配列類似性

      Belongs to the histone H2A family.
    • 翻訳後修飾

      The chromatin-associated form is phosphorylated on Thr-121 during mitosis.
      Deiminated on Arg-4 in granulocytes upon calcium entry.
      Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
      Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.
      Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
    • 細胞内局在

      Nucleus. Chromosome.
    • Target information above from: UniProt accession P04908 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 3012 Human
      • Entrez Gene: 317772 Human
      • Entrez Gene: 8335 Human
      • Entrez Gene: 8338 Human
      • Omim: 142720 Human
      • Omim: 602786 Human
      • Omim: 602797 Human
      • SwissProt: P04908 Human
      • SwissProt: P0C0S8 Human
      • SwissProt: P20671 Human
      • SwissProt: Q16777 Human
      • SwissProt: Q6FI13 Human
      • SwissProt: Q7L7L0 Human
      • SwissProt: Q8IUE6 Human
      • SwissProt: Q93077 Human
      • SwissProt: Q96KK5 Human
      • SwissProt: Q96QV6 Human
      • SwissProt: Q99878 Human
      • Unigene: 121017 Human
      • Unigene: 248174 Human
      see all
    • 別名

      • H2a 615 antibody
      • H2A antibody
      • H2A GL101 antibody
      • H2A histone family member A antibody
      • H2A.1 antibody
      • H2A.2 antibody
      • H2A/a antibody
      • H2A/m antibody
      • H2A/O antibody
      • H2A/q antibody
      • H2A1B_HUMAN antibody
      • H2AFA antibody
      • H2AFE antibody
      • H2AFL antibody
      • H2AFM antibody
      • H2AFO antibody
      • H2AFQ antibody
      • HIST1H2AE antibody
      • HIST1H2AJ antibody
      • HIST2H2AA antibody
      • HIST2H2AA3 antibody
      • HIST2H2AB antibody
      • HIST2H2AC antibody
      • Histone 1 H2ae antibody
      • Histone 2 H2aa3 antibody
      • Histone 2 H2ab antibody
      • Histone 2 H2ac antibody
      • Histone H2A type 1 B antibody
      • Histone H2A type 1 C antibody
      • Histone H2A type 1 E antibody
      • Histone H2A type 1 J antibody
      • Histone H2A type 1-B/E antibody
      • Histone H2A.2 antibody
      • Histone H2A/a antibody
      • Histone H2A/m antibody
      • MGC74460 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody - ChIP Grade (ab1764)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody - ChIP Grade (ab1764)

      IHC image of ab1764 staining Histone H2A (acetyl K5) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1764, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    プロトコール

    • Immunohistochemistry protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet download

      Download

    参考文献 (12)

    ab1764 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab1764 は 12 報の論文で使用されています。

    • Lau AC  et al. An H4K16 histone acetyltransferase mediates decondensation of the X chromosome in C. elegans males. Epigenetics Chromatin 9:44 (2016). ICC/IF . PubMed: 27777629
    • Shirakawa K  et al. Salicylate, diflunisal and their metabolites inhibit CBP/p300 and exhibit anticancer activity. Elife 5:N/A (2016). WB . PubMed: 27244239
    • Min SW  et al. Critical role of acetylation in tau-mediated neurodegeneration and cognitive deficits. Nat Med 21:1154-62 (2015). PubMed: 26390242
    • Rothbart SB  et al. An Interactive Database for the Assessment of Histone Antibody Specificity. Mol Cell 59:502-11 (2015). PubMed: 26212453
    • Hu R  et al. Zinc finger protein 668 interacts with Tip60 to promote H2AX acetylation after DNA damage. Cell Cycle 12:2033-41 (2013). PubMed: 23777805
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 14 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H2A (acetyl K5) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Colon cancer tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization
    Yes - Tween plus PBS
    Specification
    Colon cancer tissue
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jul 12 2021

    Western blot abreview for Anti-Histone H2A (acetyl K5) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (15%)
    Sample
    Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Fruit fly embryo)
    Specification
    Fruit fly embryo
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Dec 18 2013

    Western blot abreview for Anti-Histone H2A (acetyl K5) antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing (15%)
    Sample
    Mouse Cell lysate - nuclear (Mouse embryonic fibroblasts)
    Specification
    Mouse embryonic fibroblasts
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Dec 18 2013

    IHC - Wholemount abreview for Anti-Histone H2A (acetyl K5) antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Caenorhabditis elegans Tissue (Adult C. elegans whole body)
    Specification
    Adult C. elegans whole body
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Dec 18 2013

    Western blot abreview for Anti-Histone H2A (acetyl K5) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Caenorhabditis elegans Tissue lysate - whole (C. elegans embryo)
    Gel Running Conditions
    Reduced Denaturing (15%)
    Loading amount
    20 µg
    Specification
    C. elegans embryo
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Dec 18 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H2A (acetyl K5) antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Antigen retrieval step
    Heat mediated
    Sample
    Mouse Tissue sections (Mouse brain)
    Specification
    Mouse brain
    Permeabilization
    Yes - 0.3% Triton X-100
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Dec 18 2013

    Western blot abreview for Anti-Histone H2A (acetyl K5) antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (Keratinocytes)
    Loading amount
    50 µg
    Specification
    Keratinocytes
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Dec 23 2010

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H2A (acetyl K5) antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (keratinocytes)
    Specification
    keratinocytes
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.1% triton-x-100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Dec 23 2010

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H2A (acetyl K5) antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Embryonic Stem)
    Specification
    Embryonic Stem
    Fixative
    Formaldehyde
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Apr 20 2007

    Western blot abreview for Anti-Histone H2A (acetyl K5) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (osteosarcoma (U2OS))
    Specification
    osteosarcoma (U2OS)
    Blocking step
    Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Mar 14 2006

    1-10 of 14 Abreviews or Q&A

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