Anti-Histone H2A (acetyl K5) 抗体 (ab1764)
Key features and details
- Rabbit polyclonal to Histone H2A (acetyl K5)
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human, Caenorhabditis elegans, Drosophila melanogaster, Mammals, Asian toad
- Isotype: IgG
製品の概要
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製品名
Anti-Histone H2A (acetyl K5) antibody
Histone H2A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Histone H2A (acetyl K5) -
由来種
Rabbit -
特異性
Histone H2A acetylated at lysine 5. -
アプリケーション
適用あり: IHC-P, ICC/IFmore details -
種交差性
交差種: Human, Caenorhabditis elegans, Drosophila melanogaster, Mammals, Asian toad -
免疫原
Synthetic peptide corresponding to Histone H2A (acetyl K5) conjugated to Ovalbumin.
Sequence:SGRGK(Ac)QGGKYC
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ポジティブ･コントロール
- HL60 cells treated with butyrate. IHC-P: Human normal colon FFPE tissue sections.
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特記事項
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab1764 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use at an assay dependent concentration. |
ターゲット情報
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機能
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
配列類似性
Belongs to the histone H2A family. -
翻訳後修飾
The chromatin-associated form is phosphorylated on Thr-121 during mitosis.
Deiminated on Arg-4 in granulocytes upon calcium entry.
Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.
Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage. -
細胞内局在
Nucleus. Chromosome. - Information by UniProt
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参照データベース
- Entrez Gene: 191549 Caenorhabditis elegans
- Entrez Gene: 45876 Drosophila melanogaster
- Entrez Gene: 3012 Human
- Entrez Gene: 317772 Human
- Entrez Gene: 8335 Human
- Entrez Gene: 8338 Human
- Omim: 142720 Human
- Omim: 602786 Human
see all -
別名
- H2a 615 antibody
- H2A antibody
- H2A GL101 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody - ChIP Grade (ab1764)IHC image of ab1764 staining Histone H2A (acetyl K5) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1764, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H2A (acetyl K5) antibody - ChIP Grade (ab1764)ab1764 staining mouse embryonic stem cells differentiated for 7 days by immunocytochemistry. The antibody was used at a dilution of 1/200 and incubated with the cells for 1 hour. Bound antibody was detected with a FITC conjugated Goat anti-Rabbit polyclonal antibody.
DAPI counterstain is shown pseudocoloured as white on the right of the image (b). Centric heterochromatin was underacetylated in differentiated cells (arrowhead in a). A pale staining chromosome was observed at high frequencies; previoulsy shown to be the inactive female X chromosome (arrow in a).
This image is courtesy of an Abreview by Hugh Spotswood submitted on 13 February 2006.
データシートおよび資料
参考文献
ab1764 は 12 報の論文で使用されています。
- Lau AC et al. An H4K16 histone acetyltransferase mediates decondensation of the X chromosome in C. elegans males. Epigenetics Chromatin 9:44 (2016). ICC/IF . PubMed: 27777629
- Shirakawa K et al. Salicylate, diflunisal and their metabolites inhibit CBP/p300 and exhibit anticancer activity. Elife 5:N/A (2016). WB . PubMed: 27244239
- Min SW et al. Critical role of acetylation in tau-mediated neurodegeneration and cognitive deficits. Nat Med 21:1154-62 (2015). PubMed: 26390242
- Rothbart SB et al. An Interactive Database for the Assessment of Histone Antibody Specificity. Mol Cell 59:502-11 (2015). PubMed: 26212453
- Hu R et al. Zinc finger protein 668 interacts with Tip60 to promote H2AX acetylation after DNA damage. Cell Cycle 12:2033-41 (2013). PubMed: 23777805
