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Epigenetics and Nuclear Signaling Histones H2A Acetylated
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Anti-Histone H2A (acetyl K5) 抗体 (ab1764)

  • Datasheet
Reviews (9)Q&A (4)References (12)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody - ChIP Grade (ab1764)

    Key features and details

    • Rabbit polyclonal to Histone H2A (acetyl K5)
    • Suitable for: IHC-P
    • Reacts with: Human, Mammals, Asian toad
    • Isotype: IgG

    こちらの製品もご検討ください

    タンパク質
    Product image
    Recombinant Human Histone H2A (biotinylated ) protein (ab200286)
    二次抗体
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    関連製品

    製品の概要

    • 製品名

      Anti-Histone H2A (acetyl K5) antibody
      Histone H2A 一次抗体 製品一覧
    • 製品の詳細

      Rabbit polyclonal to Histone H2A (acetyl K5)
    • 由来種

      Rabbit
    • 特異性

      Histone H2A acetylated at lysine 5.
    • アプリケーション

      適用あり: IHC-Pmore details
    • 種交差性

      交差種: Human, Mammals, Asian toad
    • 免疫原

      Synthetic peptide corresponding to Histone H2A (acetyl K5) conjugated to Ovalbumin.
      Sequence:

      SGRGK(Ac)QGGKYC

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 特記事項

      ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • バッファー

      Preservative: 0.02% Sodium azide
    • Concentration information loading...
    • 精製度

      Whole antiserum
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      IgG
    • 研究分野

      • Epigenetics and Nuclear Signaling
      • Histones
      • H2A
      • Acetylated

    関連製品

    • ChIP Related Products

      • Rabbit Anti-Mouse IgG H&L (ab46540)
      • ChIP Kit (ab500)
    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Histone H2A (biotinylated ) protein (ab200286)

    アプリケーション

    Our Abpromise guarantee covers the use of ab1764 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    ターゲット情報

    • 機能

      Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
    • 配列類似性

      Belongs to the histone H2A family.
    • 翻訳後修飾

      The chromatin-associated form is phosphorylated on Thr-121 during mitosis.
      Deiminated on Arg-4 in granulocytes upon calcium entry.
      Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
      Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.
      Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
    • 細胞内局在

      Nucleus. Chromosome.
    • Target information above from: UniProt accession P04908 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 3012 Human
      • Entrez Gene: 317772 Human
      • Entrez Gene: 8335 Human
      • Entrez Gene: 8338 Human
      • Omim: 142720 Human
      • Omim: 602786 Human
      • Omim: 602797 Human
      • SwissProt: P04908 Human
      • SwissProt: P0C0S8 Human
      • SwissProt: P20671 Human
      • SwissProt: Q16777 Human
      • SwissProt: Q6FI13 Human
      • SwissProt: Q7L7L0 Human
      • SwissProt: Q8IUE6 Human
      • SwissProt: Q93077 Human
      • SwissProt: Q96KK5 Human
      • SwissProt: Q96QV6 Human
      • SwissProt: Q99878 Human
      • Unigene: 121017 Human
      • Unigene: 248174 Human
      see all
    • 別名

      • H2a 615 antibody
      • H2A antibody
      • H2A GL101 antibody
      • H2A histone family member A antibody
      • H2A.1 antibody
      • H2A.2 antibody
      • H2A/a antibody
      • H2A/m antibody
      • H2A/O antibody
      • H2A/q antibody
      • H2A1B_HUMAN antibody
      • H2AFA antibody
      • H2AFE antibody
      • H2AFL antibody
      • H2AFM antibody
      • H2AFO antibody
      • H2AFQ antibody
      • HIST1H2AE antibody
      • HIST1H2AJ antibody
      • HIST2H2AA antibody
      • HIST2H2AA3 antibody
      • HIST2H2AB antibody
      • HIST2H2AC antibody
      • Histone 1 H2ae antibody
      • Histone 2 H2aa3 antibody
      • Histone 2 H2ab antibody
      • Histone 2 H2ac antibody
      • Histone H2A type 1 B antibody
      • Histone H2A type 1 C antibody
      • Histone H2A type 1 E antibody
      • Histone H2A type 1 J antibody
      • Histone H2A type 1-B/E antibody
      • Histone H2A.2 antibody
      • Histone H2A/a antibody
      • Histone H2A/m antibody
      • MGC74460 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody - ChIP Grade (ab1764)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H2A (acetyl K5) antibody - ChIP Grade (ab1764)

      IHC image of ab1764 staining Histone H2A (acetyl K5) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1764, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    プロトコール

    • Immunohistochemistry protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet
  • 参考文献 (12)

    ab1764 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab1764 は 12 報の論文で使用されています。

    • Lau AC  et al. An H4K16 histone acetyltransferase mediates decondensation of the X chromosome in C. elegans males. Epigenetics Chromatin 9:44 (2016). ICC/IF . PubMed: 27777629
    • Shirakawa K  et al. Salicylate, diflunisal and their metabolites inhibit CBP/p300 and exhibit anticancer activity. Elife 5:N/A (2016). WB . PubMed: 27244239
    • Min SW  et al. Critical role of acetylation in tau-mediated neurodegeneration and cognitive deficits. Nat Med 21:1154-62 (2015). PubMed: 26390242
    • Rothbart SB  et al. An Interactive Database for the Assessment of Histone Antibody Specificity. Mol Cell 59:502-11 (2015). PubMed: 26212453
    • Hu R  et al. Zinc finger protein 668 interacts with Tip60 to promote H2AX acetylation after DNA damage. Cell Cycle 12:2033-41 (2013). PubMed: 23777805
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 13 Abreviews or Q&A

    Western blot abreview for Anti-Histone H2A (acetyl K5) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (15%)
    Sample
    Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Fruit fly embryo)
    Specification
    Fruit fly embryo
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 18 2013

    Western blot abreview for Anti-Histone H2A (acetyl K5) antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing (15%)
    Sample
    Mouse Cell lysate - nuclear (Mouse embryonic fibroblasts)
    Specification
    Mouse embryonic fibroblasts
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 18 2013

    IHC - Wholemount abreview for Anti-Histone H2A (acetyl K5) antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Caenorhabditis elegans Tissue (Adult C. elegans whole body)
    Specification
    Adult C. elegans whole body
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 18 2013

    Western blot abreview for Anti-Histone H2A (acetyl K5) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (15%)
    Sample
    Caenorhabditis elegans Tissue lysate - whole (C. elegans embryo)
    Specification
    C. elegans embryo
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 18 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H2A (acetyl K5) antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Antigen retrieval step
    Heat mediated
    Sample
    Mouse Tissue sections (Mouse brain)
    Specification
    Mouse brain
    Permeabilization
    Yes - 0.3% Triton X-100
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 18 2013

    Western blot abreview for Anti-Histone H2A (acetyl K5) antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (Keratinocytes)
    Loading amount
    50 µg
    Specification
    Keratinocytes
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 23 2010

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H2A (acetyl K5) antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (keratinocytes)
    Specification
    keratinocytes
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.1% triton-x-100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 23 2010

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H2A (acetyl K5) antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Embryonic Stem)
    Specification
    Embryonic Stem
    Fixative
    Formaldehyde
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 20 2007

    Western blot abreview for Anti-Histone H2A (acetyl K5) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (osteosarcoma (U2OS))
    Specification
    osteosarcoma (U2OS)
    Blocking step
    Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 14 2006

    Question

    BATCH NUMBER 68075 ORDER NUMBER unk DESCRIPTION OF THE PROBLEM Non-specific staining; general background was not high at all, however the staining is continuous along the fibers. Think: DAPI. SAMPLE Extended chromatin fibers fixed on slides. Human cell line source. PRIMARY ANTIBODY Abcam 1764-100; 1:100 in blocking buffer (BSA/PBST); overnight at 4C. Wash is 3X PBS-Tween for 5m. SECONDARY ANTIBODY Alexa 488 goat anti-rabbit, 1hr RT, wash 3X PBS-Tween for 5m. DETECTION METHOD FL-microscopy POSITIVE AND NEGATIVE CONTROLS USED paralell staining of fibers with Ab specific for H2A/H4-S1phospho. ANTIBODY STORAGE CONDITIONS 4C storage. 1:100 dilution FIXATION OF SAMPLE Formaldehyde fixation, FC 4% in PBS ANTIGEN RETRIEVAL NA PERMEABILIZATION STEP NA BLOCKING CONDITIONS BSA and PBS/Tween. 30 minutes at RT. HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes ADDITIONAL NOTES I know that this Ab is questionable (based on your website info). I'm not convinced I should waste time on "optimizing" it unless you have specific suggestions.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 13 2004

    Answer

    Thank you for your enquiry and for forwarding your completed questionnaire to us. We are very sorry to hear that you are having problem with this antibody. We have looked through your protocol and would like to make some suggestions. 1. First of all, we would like to confirm that this antibody has been tested for Immunofluorescence. If you use formaldehyde as a fixative, it is advisable to apply antigen retrieval step. This is due to the formation of methylene bridges during fixation, which cross link proteins and therefore mask antigenic sites. Please read the 'Protocol' section carefully on the Abcam website, you might get some very useful information. 2. For blocking non-specific bindings, please try to use 1-3 % BSA. 3. Since the target protein is expressed in the nucleus, it is important to permabilise the cells before the staining procedure. If you use ice-cold acetone (for 5-10 min) as a fixative, you do not need to apply permeabilizing agent since it fixes and permeabilizes the samples at the same time. 4. Take a look at these websites, you may find some useful information about the different IF procedures: http://www.bdbiosciences.com/pharmingen/protocols/Immunofluorescence_Microscopy.shtml http://www.biochem.emory.edu/labs/acorbe2/protocols13.html http://www.science.mcmaster.ca/biochem/faculty/andrews/lab/projects/methodsandprograms/labman/tc19.html http://www.kcl.ac.uk/depsta/biomedical/randall/methods/im-fluo.html Should you still have any problem, please feel free to get back to us. We can offer yiu either a new vial or a reimbursement. Good luck! Wee look forward to hearing from you soon.

    Read More

    Abcam Scientific Support

    Answered on Dec 14 2004

    1-10 of 13 Abreviews or Q&A

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