Anti-Histone H1.4 (phospho T18) 抗体 (ab3595)
Key features and details
- Rabbit polyclonal to Histone H1.4 (phospho T18)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Histone H1.4 (phospho T18) antibody
Histone H1.4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Histone H1.4 (phospho T18) -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Pig -
免疫原
Synthetic peptide corresponding to Human Histone H1.4 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab10138) -
特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab3595 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | 1/1000. Detects a band of approximately 32 kDa. | |
ICC/IF | Use at an assay dependent concentration. |
ターゲット情報
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機能
Histones H1 are necessary for the condensation of nucleosome chains into higher order structures. -
配列類似性
Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain. -
翻訳後修飾
Acetylated at Lys-26. Deacetylated at Lys-26 by SIRT1. -
細胞内局在
Nucleus. Chromosome. - Information by UniProt
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参照データベース
- Entrez Gene: 3008 Human
- Entrez Gene: 50709 Mouse
- Omim: 142220 Human
- SwissProt: P10412 Human
- SwissProt: P43274 Mouse
- Unigene: 248133 Human
- Unigene: 170587 Mouse
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別名
- H1 histone family member 4 antibody
- H1.4 antibody
- H14_HUMAN antibody
see all
画像
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Western blot - Anti-Histone H1.4 (phospho T18) antibody (ab3595)This image is courtesy of Alejandro Contreras, Baylor College of Medicine
Rabbit polyclonal to Histone H1 phospho T18 (1/1000) against recombinant Histone H1, incubated with control lysate (lanes 1, 3, 5, 7, 9, 11, 13) or insect cell lysate containing active cyclin E / CDK2 complexes (lanes 2, 4, 6, 8, 10, 12, 14).
Mutant Histone H1 proteins were made by site-directed mutagenesis of 5 phosphorylation cyclin/cdk phosphorylation concensus sites (T18, T146, T154, S172, S187). Lanes 3-12 contain mutant histone H1 with only one wild-type cyclin/cdk phosphorylation concensus site and lanes 13-14 containst mutant Histone H1 with all 5 sites mutated to Alanine.
Lanes 1-2 : wt Histone H1
Lanes 3-4 : T146A, T154A, S172A, S187A (wt site at T18)
Lanes 5-6 : T18A, T154A, S172A, S187A (wt site at T146)
Lanes 7-8 : T18A, T146A, S172A, S187A (wt site at T154)
Lanes 9-10 : T18A, T146A, T154A, S187A (wt site at S172)
Lanes 11-12: -
Immunocytochemistry/ Immunofluorescence - Anti-Histone H1.4 (phospho T18) antibody (ab3595)This image is courtesy of Dr Michael Hendzels laboratory
Rabbit polyclonal to Histone H1 (phospho T18) (1/25).
Human neuroblastoma cell line SK-N-SH cultured on coverslips were fixed in 4% paraformaldehye and then stained with ab3595 (green). The DNA stained with DAPI is shown in red. (100x magnification).
プロトコール
データシートおよび資料
参考文献 (2)
ab3595 は 2 報の論文で使用されています。
- Chen Y et al. Quantitative Mass Spectrometry Reveals that Intact Histone H1 Phosphorylations are Variant Specific and Exhibit Single Molecule Hierarchical Dependence. Mol Cell Proteomics N/A:N/A (2015). PubMed: 26209608
- Terme JM et al. Dynamics and dispensability of variant-specific histone H1 Lys-26/Ser-27 and Thr-165 post-translational modifications. FEBS Lett 588:2353-62 (2014). PubMed: 24873882