Key features and details
- Rabbit polyclonal to Histone H1.4 (phospho T18)
- Suitable for: WB, ICC/IF, ICC
- Reacts with: Human
- Isotype: IgG
製品名Anti-Histone H1.4 (phospho T18) antibody
Histone H1.4 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to Histone H1.4 (phospho T18)
アプリケーション適用あり: WB, ICC/IF, ICCmore details
交差が予測される動物種: Mouse, Rat, Cow, Pig
Synthetic peptide corresponding to Human Histone H1.4 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
精製度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab3595 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 32 kDa.|
|ICC/IF||Use at an assay dependent dilution.|
|ICC||Use at an assay dependent dilution.|
機能Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
配列類似性Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain.
翻訳後修飾Acetylated at Lys-26. Deacetylated at Lys-26 by SIRT1.
- Information by UniProt
- H1 histone family member 4 antibody
- H1.4 antibody
- H14_HUMAN antibody
Rabbit polyclonal to Histone H1 phospho T18 (1/1000) against recombinant Histone H1, incubated with control lysate (lanes 1, 3, 5, 7, 9, 11, 13) or insect cell lysate containing active cyclin E / CDK2 complexes (lanes 2, 4, 6, 8, 10, 12, 14).
Mutant Histone H1 proteins were made by site-directed mutagenesis of 5 phosphorylation cyclin/cdk phosphorylation concensus sites (T18, T146, T154, S172, S187). Lanes 3-12 contain mutant histone H1 with only one wild-type cyclin/cdk phosphorylation concensus site and lanes 13-14 containst mutant Histone H1 with all 5 sites mutated to Alanine.
Lanes 1-2 : wt Histone H1
Lanes 3-4 : T146A, T154A, S172A, S187A (wt site at T18)
Lanes 5-6 : T18A, T154A, S172A, S187A (wt site at T146)
Lanes 7-8 : T18A, T146A, S172A, S187A (wt site at T154)
Lanes 9-10 : T18A, T146A, T154A, S187A (wt site at S172)
Rabbit polyclonal to Histone H1 (phospho T18) (1/25).
Human neuroblastoma cell line SK-N-SH cultured on coverslips were fixed in 4% paraformaldehye and then stained with ab3595 (green). The DNA stained with DAPI is shown in red. (100x magnification).
ab3595 は 2 報の論文で使用されています。
- Chen Y et al. Quantitative Mass Spectrometry Reveals that Intact Histone H1 Phosphorylations are Variant Specific and Exhibit Single Molecule Hierarchical Dependence. Mol Cell Proteomics N/A:N/A (2015). PubMed: 26209608
- Terme JM et al. Dynamics and dispensability of variant-specific histone H1 Lys-26/Ser-27 and Thr-165 post-translational modifications. FEBS Lett 588:2353-62 (2014). PubMed: 24873882