Application
Western blot
Sample
Spermophilus tridecemlineatus Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (12.5%)
Loading amount
15 µg
Specification
Liver
Blocking step
BSA as blocking agent for 7 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST with 0.3% BSA
Dilution
1/5000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 22 kDa Non-specific: many kDa
Positive control
Squirrel liver whole tissue homogenate, cytosolic fraction, mitochondrial fraction, and nuclear fraction prepared in RIPA.
Additional data
Additional Notes
Lane 1=15ug Whole Sq liver tissue
Lane 2=15ug Sg liver cytosolic fraction
Lane 3=15ug Sq liver mitochondrial fraction
Lane 4=15ug Sq liver nuclear fraction
MR. James Bjork
Verified customer
投稿 Aug 24 2015