Application
Western blot
Sample
Human Cell lysate - whole cell (MRC5VA)
Loading amount
15 µg
Specification
MRC5VA
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Milk
Secondary antibody
Name
Non-Abcam antibody was used: DAKO Goat Anti Rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
10 second(s)
Bands
Specific: ~30 kDa Non-specific: 70 kDa
Additional data
Additional Notes
Used at the specified concentration, 1/500 and it was very strong, will now use at 1/1000 or 1/5000
Abcam response
Histone extraction might get rid of the higher bands.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Sep 01 2009