Anti-Histone H1.1 抗体 (ab17584)
Key features and details
- Rabbit polyclonal to Histone H1.1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Histone H1.1 antibody
Histone H1.1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Histone H1.1 -
由来種
Rabbit -
特異性
Reacts with recombinant Human H1.1, not with other Human H1 isoforms or core histones. Does not react with histone H1 acid extracted from cell-lines such as breast cancer cell line T47D, because H1.1 is not expressed in this cell line. H1.1 is not expressed in many cell lines, as has been previously reported (Meergans et al, 1997) -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide:
SAAPEKPLAGKKAKKPAKAAAASKKKPA-C
, corresponding to amino acids 12-39 of Human Histone H1.1. -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab17584の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 21.7 kDa). Human H1.1 is not present in many human cell lines. Cross reacts with a band of approx. 52kDa and 40kDa in nuclear extracts of T47D cell line.
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| 特記事項 |
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WB
1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 21.7 kDa). Human H1.1 is not present in many human cell lines. Cross reacts with a band of approx. 52kDa and 40kDa in nuclear extracts of T47D cell line. |
ターゲット情報
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関連性
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. The C-terminal domain is the primary determinant of histone H1 binding to chromatin in vivo. The C-terminal region also shows the most differences in aa sequence between the H1 histone variants H1.0/1/2/3/4/5. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 3024 Human
- NCBI: NP_005316 Human
- SwissProt: Q02539 Human
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別名
- H1 histone family member 1 antibody
- H1.1 antibody
- H1F1 antibody
see all
画像
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Western blot - Anti-Histone H1.1 antibody (ab17584)This image is courtesy of Dr Albert Jordan, Center for Genomic Regulation (CRG).Rabbit polyclonal to Histone H1.1 (ab17584) against recombinant human H1 isoforms H1.0 to H1.5 as depicted in the figure or a chromatin preparation (ch) from a breast cancer cell line.Notice that H1.1 is not present/detected in the chromatin preparation.Recombinant isoforms courtesy of Dr. Nicole HappelRabbit polyclonal to Histone H1.1 (ab17584) against recombinant human H1 isoforms H1.0 to H1.5 as depicted in the figure or a chromatin preparation (ch) from a breast cancer cell line.Notice that H
データシートおよび資料
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Datasheet download
参考文献 (3)
ab17584 は 3 報の論文で使用されています。
- Johann DJ et al. Approaching solid tumor heterogeneity on a cellular basis by tissue proteomics using laser capture microdissection and biological mass spectrometry. J Proteome Res 8:2310-8 (2009). WB ; Human . PubMed: 19284784
- Happel N et al. H1 histone subtype constitution and phosphorylation state of the ageing cell system of human peripheral blood lymphocytes. Exp Gerontol 43:184-99 (2008). PubMed: 18234461
- Sancho M et al. Depletion of human histone H1 variants uncovers specific roles in gene expression and cell growth. PLoS Genet 4:e1000227 (2008). WB ; Human . PubMed: 18927631
