Anti-Histone H1 (phospho ) 抗体 (ab4270)
Key features and details
- Rabbit polyclonal to Histone H1 (phospho )
- Suitable for: ICC, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Histone H1 (phospho ) antibody
Histone H1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Histone H1 (phospho ) -
由来種
Rabbit -
特異性
Recognizes phosphorylated histone H1 -
アプリケーション
適用あり: ICC, WBmore details -
種交差性
交差種: Human -
免疫原
Hyper-phosphorylated isoform of tetrahymena Histone H1
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特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.035% Sodium azide
Constituents: 1.3% Tris glycine, 30% Glycerol, 0.667% Sodium chloride -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab4270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 32 kDa. Additional proteins at 66 kD and 100 kD were detected. It detected Histone H1 from acid extracts of colcemid treated HeLa cells but not untreated HeLa cells. Acid extracts from colcemid treated and untreated HeLa cell were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho- Histone H1 (2 µg/ml). |
ターゲット情報
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機能
Histones H1 are necessary for the condensation of nucleosome chains into higher order structures. -
配列類似性
Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain. -
翻訳後修飾
Acetylated at Lys-26. Deacetylated at Lys-26 by SIRT1. -
細胞内局在
Nucleus. Chromosome. - Information by UniProt
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参照データベース
- Entrez Gene: 3008 Human
- Omim: 142711 Human
- SwissProt: P10412 Human
- SwissProt: P16401 Human
- Unigene: 131956 Human
- Unigene: 248133 Human
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別名
- H1 histone family antibody
- H14_HUMAN antibody
- H1F5 antibody
see all
画像
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Immunocytochemistry - Anti-Histone H1 (phospho ) antibody (ab4270)This image is courtesy of Dr Michael Hendzels Laboratory
Rabbit polyclonal to Histone H1 (phospho) (1/100).
Human neuroblastoma cell line SK-N-SH cultured on coverslips were fixed in 4% paraformaldehye and then stained with ab4270 (green). The DNA stained with DAPI is shown in red. (100x magnification).
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Western blot - Anti-Histone H1 (phospho ) antibody (ab4270)All lanes : Anti-Histone H1 (phospho ) antibody (ab4270) at 2 µg/ml
Lane 1 : Colcemid treated HeLa cell lysate
Lane 2 : Untreated HeLa cell lysate
Secondary
Lane 1 : Goat anti-rabbit
secondary antibody labeled with HRP
Lane 2 : Goat anti-rabbit
secondary antibody labeled with HRP.
Developed using the ECL technique.
プロトコール
データシートおよび資料
参考文献 (6)
ab4270 は 6 報の論文で使用されています。
- Murthy T et al. Cyclin-dependent kinase 1 (CDK1) and CDK2 have opposing roles in regulating interactions of splicing factor 3B1 with chromatin. J Biol Chem 293:10220-10234 (2018). PubMed: 29764937
- Zhang B et al. Silybin attenuates LPS-induced lung injury in mice by inhibiting NF-?B signaling and NLRP3 activation. Int J Mol Med 39:1111-1118 (2017). PubMed: 28350048
- Büchner A et al. Sumoylation of p35 modulates p35/cyclin-dependent kinase (Cdk) 5 complex activity. Neuromolecular Med 17:12-23 (2015). PubMed: 25391294
- Chien WW et al. S-phase lengthening induced by p16(INK4a) overexpression in malignant cells with wild-type pRb and p53. Cell Cycle 9: (2010). WB ; Human . PubMed: 20703084
- Ingemarsdotter CK et al. Low-dose paclitaxel synergizes with oncolytic adenoviruses via mitotic slippage and apoptosis in ovarian cancer. Oncogene 29:6051-63 (2010). WB ; Human . PubMed: 20729921
- Tang X et al. A novel ATM-dependent pathway regulates protein phosphatase 1 in response to DNA damage. Mol Cell Biol 28:2559-66 (2008). Flow Cyt ; Human . PubMed: 18250156
