Anti-Histone H1 (phospho ) 抗体 (ab4270)

Reviews (1)Q&A (1)References (6)
Immunocytochemistry/ Immunofluorescence - Anti-Histone H1 (phospho ) antibody (ab4270)
  • Western blot - Anti-Histone H1 (phospho ) antibody (ab4270)

Key features and details

  • Rabbit polyclonal to Histone H1 (phospho )
  • Suitable for: ICC, ICC/IF, Flow Cyt, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Histone H1 (phospho ) antibody

  • 製品の詳細

    Rabbit polyclonal to Histone H1 (phospho )

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC, ICC/IF, Flow Cyt, WBmore details

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Hyper-phosphorylated isoform of tetrahymena Histone H1

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.035% Sodium azide
    Constituents: 1.3% Tris glycine, 30% Glycerol, 0.667% Sodium chloride
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab4270の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration. PubMed: 18250156

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB (1)
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 32 kDa. Additional proteins at 66 kD and 100 kD were detected. It detected Histone H1 from acid extracts of colcemid treated HeLa cells but not untreated HeLa cells. Acid extracts from colcemid treated and untreated HeLa cell were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho- Histone H1 (2 µg/ml).
特記事項
ICC
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration. PubMed: 18250156

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 32 kDa. Additional proteins at 66 kD and 100 kD were detected. It detected Histone H1 from acid extracts of colcemid treated HeLa cells but not untreated HeLa cells. Acid extracts from colcemid treated and untreated HeLa cell were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho- Histone H1 (2 µg/ml).

ターゲット情報

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H1 (phospho ) antibody (ab4270)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H1 (phospho ) antibody (ab4270)This image is courtesy of Dr Michael Hendzels Laboratory

    Rabbit polyclonal to Histone H1 (phospho) (1/100).

    Human neuroblastoma cell line SK-N-SH cultured on coverslips were fixed in 4% paraformaldehye and then stained with ab4270 (green). The DNA stained with DAPI is shown in red. (100x magnification).

  • Western blot - Anti-Histone H1 (phospho ) antibody (ab4270)
    Western blot - Anti-Histone H1 (phospho ) antibody (ab4270)
    All lanes : Anti-Histone H1 (phospho ) antibody (ab4270) at 2 µg/ml

    Lane 1 : Colcemid treated HeLa cell lysate
    Lane 2 : Untreated HeLa cell lysate

    Secondary
    Lane 1 : Goat anti-rabbit
    secondary antibody labeled with HRP
    Lane 2 : Goat anti-rabbit
    secondary antibody labeled with HRP.

    Developed using the ECL technique.

参考文献 (6)

ab4270 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab4270 は 6 報の論文で使用されています。

  • Murthy T  et al. Cyclin-dependent kinase 1 (CDK1) and CDK2 have opposing roles in regulating interactions of splicing factor 3B1 with chromatin. J Biol Chem 293:10220-10234 (2018). PubMed: 29764937
  • Zhang B  et al. Silybin attenuates LPS-induced lung injury in mice by inhibiting NF-?B signaling and NLRP3 activation. Int J Mol Med 39:1111-1118 (2017). PubMed: 28350048
  • Büchner A  et al. Sumoylation of p35 modulates p35/cyclin-dependent kinase (Cdk) 5 complex activity. Neuromolecular Med 17:12-23 (2015). PubMed: 25391294
  • Chien WW  et al. S-phase lengthening induced by p16(INK4a) overexpression in malignant cells with wild-type pRb and p53. Cell Cycle 9: (2010). WB ; Human . PubMed: 20703084
  • Ingemarsdotter CK  et al. Low-dose paclitaxel synergizes with oncolytic adenoviruses via mitotic slippage and apoptosis in ovarian cancer. Oncogene 29:6051-63 (2010). WB ; Human . PubMed: 20729921
  • Tang X  et al. A novel ATM-dependent pathway regulates protein phosphatase 1 in response to DNA damage. Mol Cell Biol 28:2559-66 (2008). Flow Cyt ; Human . PubMed: 18250156

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-Histone H1 (phospho) antibody

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Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Parotid gland)
Loading amount
100 µg
Specification
Parotid gland
Treatment
5Gy gamma irradiation
Gel Running Conditions
Non-reduced Denaturing (12% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1500µg/mL · Temperature: 20°C
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Dr. Kirsten Limesand

Verified customer

投稿 Nov 16 2010

Question

I am wondering if you can tell me more about this antibody:
Anti-Histone H1 (phospho) antibody (ab4270)
Specifically, what phosphorylated residue does the antibody recognize?

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Answer



Concerningab4270, Histone H1, phosphorylated PabRabbit x:



Thisantibody is produced by using hyper-phosphorylated histone H1 as the immunogen. The number and location of phosphorylation sites that thisantibody recognizes have not been determined. Thisantibody has been used to monitor passage through mitosis and the signal is maximal at metaphase. The presence ofhyper-phosphorylated histone H1has also been used as a measure of cdck1 activity.



There is no single specific phosphorylation site on H1 that is required for recognition by ab4270.

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