Anti-Histone H1 (phospho ) 抗体 (ab4270)
Key features and details
- Rabbit polyclonal to Histone H1 (phospho )
- Suitable for: ICC, ICC/IF, Flow Cyt, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Histone H1 (phospho ) antibody -
製品の詳細
Rabbit polyclonal to Histone H1 (phospho ) -
由来種
Rabbit -
アプリケーション
適用あり: ICC, ICC/IF, Flow Cyt, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Hyper-phosphorylated isoform of tetrahymena Histone H1
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.035% Sodium azide
Constituents: 1.3% Tris glycine, 30% Glycerol, 0.667% Sodium chloride -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab4270の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration. PubMed: 18250156
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | (1) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 32 kDa. Additional proteins at 66 kD and 100 kD were detected. It detected Histone H1 from acid extracts of colcemid treated HeLa cells but not untreated HeLa cells. Acid extracts from colcemid treated and untreated HeLa cell were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho- Histone H1 (2 µg/ml).
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| 特記事項 |
|---|
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ICC
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. PubMed: 18250156 ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 32 kDa. Additional proteins at 66 kD and 100 kD were detected. It detected Histone H1 from acid extracts of colcemid treated HeLa cells but not untreated HeLa cells. Acid extracts from colcemid treated and untreated HeLa cell were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho- Histone H1 (2 µg/ml). |
ターゲット情報
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機能
Histones H1 are necessary for the condensation of nucleosome chains into higher order structures. -
配列類似性
Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain. -
翻訳後修飾
Acetylated at Lys-26. Deacetylated at Lys-26 by SIRT1. -
細胞内局在
Nucleus. Chromosome. - Information by UniProt
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参照データベース
- Entrez Gene: 3008 Human
- Entrez Gene: 50709 Mouse
- Omim: 142711 Human
- SwissProt: P10412 Human
- SwissProt: P16401 Human
- SwissProt: P43274 Mouse
- SwissProt: P43276 Mouse
- Unigene: 248133 Human
see all -
別名
- H1 histone family antibody
- H14_HUMAN antibody
- H1F5 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H1 (phospho ) antibody (ab4270)This image is courtesy of Dr Michael Hendzels Laboratory
Rabbit polyclonal to Histone H1 (phospho) (1/100).
Human neuroblastoma cell line SK-N-SH cultured on coverslips were fixed in 4% paraformaldehye and then stained with ab4270 (green). The DNA stained with DAPI is shown in red. (100x magnification).
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Western blot - Anti-Histone H1 (phospho ) antibody (ab4270)All lanes : Anti-Histone H1 (phospho ) antibody (ab4270) at 2 µg/ml
Lane 1 : Colcemid treated HeLa cell lysate
Lane 2 : Untreated HeLa cell lysate
Secondary
Lane 1 : Goat anti-rabbit
secondary antibody labeled with HRP
Lane 2 : Goat anti-rabbit
secondary antibody labeled with HRP.
Developed using the ECL technique.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (7)
ab4270 は 7 報の論文で使用されています。
- Murthy T et al. Cyclin-dependent kinase 1 (CDK1) and CDK2 have opposing roles in regulating interactions of splicing factor 3B1 with chromatin. J Biol Chem 293:10220-10234 (2018). PubMed: 29764937
- Zhang B et al. Silybin attenuates LPS-induced lung injury in mice by inhibiting NF-?B signaling and NLRP3 activation. Int J Mol Med 39:1111-1118 (2017). PubMed: 28350048
- Lei X et al. Neuroprotective effects of lycopene pretreatment on transient global cerebral ischemia-reperfusion in rats: The role of the Nrf2/HO-1 signaling pathway. Mol Med Rep 13:412-8 (2016). PubMed: 26572165
- Büchner A et al. Sumoylation of p35 modulates p35/cyclin-dependent kinase (Cdk) 5 complex activity. Neuromolecular Med 17:12-23 (2015). PubMed: 25391294
- Chien WW et al. S-phase lengthening induced by p16(INK4a) overexpression in malignant cells with wild-type pRb and p53. Cell Cycle 9: (2010). WB ; Human . PubMed: 20703084
- Ingemarsdotter CK et al. Low-dose paclitaxel synergizes with oncolytic adenoviruses via mitotic slippage and apoptosis in ovarian cancer. Oncogene 29:6051-63 (2010). WB ; Human . PubMed: 20729921
- Tang X et al. A novel ATM-dependent pathway regulates protein phosphatase 1 in response to DNA damage. Mol Cell Biol 28:2559-66 (2008). Flow Cyt ; Human . PubMed: 18250156
