Anti-Histone Core 抗体 (ab7832)

Reviews (1)Q&A (3)References (2)

Key features and details

  • Sheep polyclonal to Histone Core
  • Reacts with: Mouse, Cow, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Histone Core antibody

  • 製品の詳細

    Sheep polyclonal to Histone Core

  • 由来種

    Sheep

  • 特異性

    The antibody binds to H1, H2B, H3 and H4. There is no evidence to suggest that the antibody can bind to H2A.

  • 種交差性

    交差種: Mouse, Cow, Human

  • 免疫原

    Other Immunogen Type. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Tonsil, colon carcinoma and any other human tissues.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Concentration information loading...

  • 精製度

    Protein G purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

関連製品

ターゲット情報

  • 関連性

    In diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. Histones are believed to be regularly arranged in the deep grove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers. The histone core protein is an octamer consisting of 2 subunits of each of histone 2A, 2B, 3 and 4. In conjunction with the linker histone H1 and the nonhistone chromosomal proteins, it is responsible for the binding and compacting of DNA into chromatin. The sequences of the core histones are highly conserved from species to species.

  • 細胞内局在

    Nuclear

参考文献 (2)

ab7832 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7832 は 2 報の論文で使用されています。

  • Zheng S  et al. MIR31HG promotes cell proliferation and invasion by activating the Wnt/ß-catenin signaling pathway in non-small cell lung cancer. Oncol Lett 17:221-229 (2019). PubMed: 30655759
  • Li Y  et al. Characterization of nucleohistone and nucleoprotamine components in the mature human sperm nucleus. Asian J Androl 10:535-41 (2008). ICC/IF ; Human . PubMed: 18478156

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Western blot abreview for Anti-Histone Core antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HL60)
Loading amount
10 µg
Specification
HL60
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Abcam user community

Verified customer

投稿 Jul 13 2007

Question

I was wondering if ab7832 recognizes histone H2A. The data sheet does not list it as being recognized by the anitbody.

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Answer

The antibody (ab7832 -- Sheep polyclonal to Histone Core)binds to H1, H2B, H3 and H4; however, there is no evidence to suggest that the antibody can bind to H2A.

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Question

I am looking for antibody to recognize the core histones in Drosophila melanogaster. Has this antibody been tested in this species?

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Answer

This antibody has not been tested specifically in Drosophila, but it works in both animal and plant tissue, so I would expect it to work in Drosophila. Core histones are highly conserved.

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Question

I'm interested in finding an antibody that will recognize the HFD of S. cerevisiae H4 expressed in E. coli. Will this antibody do this?

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Answer

This antibody has not been tested in budding yeast, however as histones are highly conserved across species, it is quite likely that the antibody will react with yeast histones. As the antibody is raised against a mixture of histones, I think it likely that it will recognise the histone fold domain.

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