製品の概要

  • 製品名

    Histone Acetyltransferase Inhibitor Screening Assay Kit
    Histone acetyltransferase キット 製品一覧
  • 検出方法

    Fluorescent
  • 再現性

    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    HAT 16 3.2%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    HAT 16 4.4%
  • アッセイタイプ

    Enzyme activity
  • 製品の概要

     Histone Acetyltransferase Inhibitor Screening Kit (ab133099) provides a fast, fluorescence-based method for evaluating pCAF HAT inhibitors.

    The procedure is based on the ability of 7-diethylamino-3-(4'-maleimidylphenyl)-4-methylcoumarin (CPM) to react with free thiol grious present after the acetylation reaction. The reaction of the CPM reacts these free thiol groups forms a highly fluorescent product that is detected using excitation and emission wavelengths of 360-390 nm and 450-470 nm, respectively.

  • 試験プラットフォーム

    Microplate reader

製品の特性

画像

  • An example of a known pCAF HAT inhibitor, garcinol, interfering with the assay.

プロトコール

参考文献

ab133099 has not yet been referenced specifically in any publications.

レビューと Q&A

Answer

Hello! The two kits ab115102 and ab113476 should be suitable for use in fish, while ab133099 is designed to study inhibitors and so fish is not suitable. Please let me know if you have further questions.

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