Application
Western blot
Sample
Escherichia coli Purified protein (Gen. Acc. HV1: KR066902.1, HV2: KR066903.1)
Gel Running Conditions
Reduced Denaturing (20)
Loading amount
10 µg
Specification
Gen. Acc. HV1: KR066902.1, HV2: KR066903.1
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 22°C
Other product details
Incubation time
17 hour(s) and 0 minute(s) · Diluent: 0,1 % (w/v) Milk-TTBS-Lösung
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Anti-mouse IgG, HRP-linked Antibody
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ChemiLumi
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: ~14 kDa Non-specific: >66 kDa
Positive control
Bands to concentrations down to 0,1µg/band.
Native samples should more spots, properbly oligomers or Konfomers
Additional data
Additional Notes
Antibody propable detects PKP-motif with C-terminal region of hirudin varaints. HV1 and HV2 testet in the trials were able to detect down to 0,1µg/band. Detektion occured in the range of 30 min. Additional fine tuning may detect lower amounts per band
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Phil Lukas
Verified customer
投稿 May 24 2019