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Anti-HIRA/HIR 抗体 (ab20655)

Reviews (3)Q&A (4)References (8)
Western blot - Anti-HIRA/HIR antibody (ab20655)

    Key features and details

    • Rabbit polyclonal to HIRA/HIR
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-HIRA/HIR antibody [EPR7416] (ab129169)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-HIRA/HIR antibody
      HIRA/HIR 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to HIRA/HIR

    • 由来種

      Rabbit

    • 特異性

      ab20655 is able to recognise a band at 115kDa corresponding to the long isoform of HIRA/HIR and a band at 89kDa, corresponding to the short isoform of HIRA/HIR in HepG2 nuclear extract.

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse, Rat, Dog

    • 免疫原

      Synthetic peptide corresponding to Human HIRA/HIR aa 1000 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
      Database link: P54198
      (Peptide available as ab22456, ab22457)

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab20655の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB (2)
    Use a concentration of 1 µg/ml. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).

    Detects a band of approximately 89 kDa, corresponding to the short isoform of HIRA/HIR.
    特記事項
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).

    Detects a band of approximately 89 kDa, corresponding to the short isoform of HIRA/HIR.

    ターゲット情報

    • 機能

      Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.

    • 組織特異性

      Expressed at high levels in kidney, pancreas and skeletal muscle and at lower levels in brain, heart, liver, lung, and placenta.

    • 配列類似性

      Belongs to the WD repeat HIR1 family.
      Contains 8 WD repeats.

    • 発生段階

      Expressed during embryogenesis.

    • 翻訳後修飾

      Sumoylated.
      Phosphorylated by CDK2/CCNA1 and CDK2/CCNE1 on Thr-555 in vitro. Also phosphorylated on Thr-555 and Ser-687 in vivo.

    • 細胞内局在

      Nucleus. Nucleus > PML body. Primarily, though not exclusively, localized to the nucleus. Localizes to PML bodies immediately prior to onset of senescence.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • DGCR1 antibody
      • DiGeorge critical region gene 1 antibody
      • HIR antibody
      • HIR histone cell cycle regulation defective homolog A antibody
      • HIRA antibody
      • HIRA protein antibody
      • HIRA_HUMAN antibody
      • Histone cell cycle regulator antibody
      • Protein HIRA antibody
      • TUP 1 antibody
      • TUP1 like enhancer of split protein 1 antibody
      • TUP1-like enhancer of split protein 1 antibody
      • TUPLE1 antibody
      see all

    画像

    • Western blot - Anti-HIRA/HIR antibody (ab20655)
      Western blot - Anti-HIRA/HIR antibody (ab20655)
      ab20655 at 10 µg + HepG2 nuclear extract lysate at 10 µg

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 89 kDa
      Observed band size: 115 kDa why is the actual band size different from the predicted?
      Additional bands at: 89 kDa (possible isoform)



      ab20655 is able to recognise a band at 115kDa corresponding to the long isoform of HIRA/HIR and a band at 89kDa, corresponding to the short isoform of HIRA/HIR.

    参考文献 (8)

    ab20655 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab20655 は 8 報の論文で使用されています。

    • Saavedra F  et al. JMJD1B, a novel player in histone H3 and H4 processing to ensure genome stability. Epigenetics Chromatin 13:6 (2020). PubMed: 32070414
    • Zhang Q  et al. Role of lncRNA uc.457 in the differentiation and maturation of cardiomyocytes. Mol Med Rep 19:4927-4934 (2019). PubMed: 30957182
    • Cohen C  et al. Promyelocytic leukemia (PML) nuclear bodies (NBs) induce latent/quiescent HSV-1 genomes chromatinization through a PML NB/Histone H3.3/H3.3 Chaperone Axis. PLoS Pathog 14:e1007313 (2018). PubMed: 30235352
    • Rivera C  et al. Methylation of histone H3 lysine 9 occurs during translation. Nucleic Acids Res 43:9097-106 (2015). PubMed: 26405197
    • Majumder A  et al. Histone Chaperone HIRA in Regulation of Transcription Factor RUNX1. J Biol Chem 290:13053-63 (2015). PubMed: 25847244
    • O'Sullivan RJ  et al. Rapid induction of alternative lengthening of telomeres by depletion of the histone chaperone ASF1. Nat Struct Mol Biol 21:167-74 (2014). WB ; Human . PubMed: 24413054
    • Lewis PW  et al. Daxx is an H3.3-specific histone chaperone and cooperates with ATRX in replication-independent chromatin assembly at telomeres. Proc Natl Acad Sci U S A 107:14075-80 (2010). ChIP ; Mouse . PubMed: 20651253
    • Dutta D  et al. Regulation of angiogenesis by histone chaperone HIRA-mediated incorporation of lysine 56-acetylated histone H3.3 at chromatin domains of endothelial genes. J Biol Chem 285:41567-77 (2010). WB ; Mouse . PubMed: 21041298

    レビューと Q&A

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    レビューを送る 質問を送る

    1-7 of 7 Abreviews or Q&A

    Western blot abreview for Anti-HIRA antibody

     Inconclusive
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    10 cells
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris gel, precasted)
    Sample
    Zebrafish Tissue lysate - whole (10 whole embryo lysate, deyolked)
    Specification
    10 whole embryo lysate, deyolked
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Mr. Maximilian Krause

    Verified customer

    投稿 Feb 03 2014

    Western blot abreview for Anti-HIRA antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Hela HEK293 NIH-3T3 MEF)
    Loading amount
    20 µg
    Specification
    Hela HEK293 NIH-3T3 MEF
    Gel Running Conditions
    Reduced Denaturing (Precise protein gel 8-16%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Mr. Antibody Solutions

    Verified customer

    投稿 Jun 16 2009

    Immunocytochemistry/ Immunofluorescence abreview for Anti-HIRA antibody

     Average
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa and Indian muntjac)
    Specification
    HeLa and Indian muntjac
    Fixative
    Paraformaldehyde
    Read More

    DR. Kirk Mcmanus

    Verified customer

    投稿 Apr 20 2006

    Question

    Thank you for your detail information. It is useful and good to know that. Then I would like to try the anti-antibody (ab20555) which you recommended.Thank you very much

    Read More
    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 11111111111.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Question

    I definitely want to give it (ab20655) a shot. However, do you know why the predicted size of HIRA is different detected by two different antibodies? In the data sheet, the HIRA detected by ab20555 is 89 kDa, while it is 112 kDa byAb129169.

    Read More
    Answer



    I have checked SwissProte, and it seems that there are two isoforms: one with 112kDa, and one with 89 kDa, which would match perfectly. You canfind the database link on ourdatasheet.

    In addition, I found that we received almost theexact same question in 2006, and here is what my colleague answered: "I have been in touch with my chromatin business development colleague and the size of the band that this antibody detects (89KDa) can be explained by the splice variant shown at the following SwissProt link:

    http://us.expasy.org/uniprot/P54198-2

    At present we have not been able to detect full length HIRA: 112KDa as defined by the SwissProt reference P54198."

    This answer can be found within the Scientific Support Tag on our datasheet.

    Please let me know if you still want to try the ab20655 Anti-HIRA antibody.

    Read More

    Question

    Dear Sir/Madam, I am interested in having an antibody against HIRA. I have found your HIRA antibody (ab20655), but I slightly confused because on the picture shown in your website, the size of the bands detected don't match the size of published HIRA. Am I missing something? Please let me know if you can help. Many thanks. Best regards.

    Read More
    Answer

    Thank you for your enquiry. I have been in touch with my chromatin business development colleague and the size of the band that this antibody detects (89KDa) can be explained by the splice variant shown at the following SwissProt link: http://us.expasy.org/uniprot/P54198-2 At present we have not been able to detect full length HIRA: 112KDa as defined by the SwissProt reference P54198. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    Dear Sir/Madam, I am interested in having an antibody against HIRA. I have found your HIRA antibody (ab20655), but I slightly confused because on the picture shown in your website, the size of the bands detected don't match the size of published HIRA. Am I missing something? Please let me know if you can help.

    Read More
    Answer

    Thank you for your enquiry. Ab20655 recognises a band at 89kDa which corresponds to the short isoform of HIRA. This band was specifically blocked by the immunising peptide. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

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