Anti-HIP55 抗体 (ab2836)
Key features and details
- Goat polyclonal to HIP55
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-HIP55 antibody
HIP55 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to HIP55 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Dog
-
免疫原
Synthetic peptide:
AANLSRNGPALQE
, corresponding to N terminal amino acids 2-14 of Human HIP55. -
特記事項
GenBank Accession Number – NP_054782.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab2836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 49 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
-
機能
Actin-binding adapter protein. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Its association with dynamin suggests that it may also connect the actin cytoskeleton to endocytic function. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. Binds to F-actin but is not involved in actin polymerization, capping or bundling. Does not bind G-actin. -
配列類似性
Belongs to the ABP1 family.
Contains 1 ADF-H domain.
Contains 1 SH3 domain. -
ドメイン
The SH3 domain mediates interaction with SHANK2, SHANK3 and PRAM1. -
翻訳後修飾
Degraded by caspases during apoptosis. -
細胞内局在
Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cortical cytoskeleton. Associates with lamellipodial actin. - Information by UniProt
-
参照データベース
- Entrez Gene: 28988 Human
- Entrez Gene: 13169 Mouse
- Entrez Gene: 83527 Rat
- Omim: 610106 Human
- SwissProt: Q9UJU6 Human
- SwissProt: Q62418 Mouse
- SwissProt: Q9JHL4 Rat
- Unigene: 436500 Human
see all -
別名
- ABP1 antibody
- Actin-binding protein 1 antibody
- Cervical mucin associated protein antibody
see all
画像
-
Western blot - Anti-HIP55 antibody (ab2836)Anti-HIP55 antibody (ab2836) at 1 µg/ml + 3T3 cell lysate at 35 µg
Predicted band size: 49 kDa -
Western blot - Anti-HIP55 antibody (ab2836)ab2836 staining (1µ g/ml) of Jurkat lysate (RIPA buffer, 35µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
プロトコール
データシートおよび資料
参考文献 (5)
ab2836 は 5 報の論文で使用されています。
- Okur E & Yerlikaya A A novel and effective inhibitor combination involving bortezomib and OTSSP167 for breast cancer cells in light of label-free proteomic analysis. Cell Biol Toxicol N/A:N/A (2018). PubMed: 29948483
- Lenos KJ et al. Stem cell functionality is microenvironmentally defined during tumour expansion and therapy response in colon cancer. Nat Cell Biol 20:1193-1202 (2018). PubMed: 30177776
- Santos MS et al. Protein interactions of the vesicular glutamate transporter VGLUT1. PLoS One 9:e109824 (2014). PubMed: 25334008
- Hepper I et al. The mammalian actin-binding protein 1 is critical for spreading and intraluminal crawling of neutrophils under flow conditions. J Immunol 188:4590-601 (2012). ICC/IF ; Human . PubMed: 22450813
- Skelton TS et al. Downregulation of RhoA and changes in T cell cytoskeleton correlate with the abrogation of allograft rejection. Transpl Immunol 23:185-93 (2010). IHC-Fr ; Rat . PubMed: 20619345
