製品の概要
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製品名Anti-HIP55 antibody
HIP55 一次抗体 製品一覧 -
製品の詳細Goat polyclonal to HIP55
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由来種Goat
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アプリケーション適用あり: ICC/IF, ELISA, WBmore details
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種交差性交差種: Mouse, Human
交差が予測される動物種: Rat, Dog
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免疫原
Synthetic peptide:
AANLSRNGPALQE
, corresponding to N terminal amino acids 2-14 of Human HIP55. -
ポジティブ･コントロール
- Jurkat and 3T3 lysates.
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特記事項GenBank Accession Number – NP_054782.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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バッファーpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab2836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. PubMed: 22450813 | |
| ELISA | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 49 kDa). |
ターゲット情報
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機能Actin-binding adapter protein. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Its association with dynamin suggests that it may also connect the actin cytoskeleton to endocytic function. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. Binds to F-actin but is not involved in actin polymerization, capping or bundling. Does not bind G-actin.
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配列類似性Belongs to the ABP1 family.
Contains 1 ADF-H domain.
Contains 1 SH3 domain. -
ドメインThe SH3 domain mediates interaction with SHANK2, SHANK3 and PRAM1.
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翻訳後修飾Degraded by caspases during apoptosis.
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細胞内局在Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cortical cytoskeleton. Associates with lamellipodial actin.
- Information by UniProt
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参照データベース
- Entrez Gene: 28988 Human
- Entrez Gene: 13169 Mouse
- Entrez Gene: 83527 Rat
- Omim: 610106 Human
- SwissProt: Q9UJU6 Human
- SwissProt: Q62418 Mouse
- SwissProt: Q9JHL4 Rat
- Unigene: 436500 Human
see all -
別名
- ABP1 antibody
- Actin-binding protein 1 antibody
- Cervical mucin associated protein antibody
see all
画像
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Western blot - Anti-HIP55 antibody (ab2836)Anti-HIP55 antibody (ab2836) at 1 µg/ml + 3T3 cell lysate at 35 µg
Predicted band size: 49 kDa -
Western blot - Anti-HIP55 antibody (ab2836)ab2836 staining (1µ g/ml) of Jurkat lysate (RIPA buffer, 35µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
参考文献
This product has been referenced in:
- Santos MS et al. Protein interactions of the vesicular glutamate transporter VGLUT1. PLoS One 9:e109824 (2014). Read more (PubMed: 25334008) »
- Hepper I et al. The mammalian actin-binding protein 1 is critical for spreading and intraluminal crawling of neutrophils under flow conditions. J Immunol 188:4590-601 (2012). ICC/IF ; Human . Read more (PubMed: 22450813) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"