Anti-HIF3 alpha/IPAS 抗体 (ab2165)

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製品の概要

  • 製品名

    Anti-HIF3 alpha/IPAS antibody
    HIF3 alpha/IPAS 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to HIF3 alpha/IPAS

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Mouse
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide corresponding to Rabbit HIF3 alpha/IPAS aa 581-592 conjugated to keyhole limpet haemocyanin.
    Sequence:

    EIKPPKRSPRLE

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項

    Previously labelled as HIF3 alpha. 

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1600. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
IHC-P Use at an assay dependent concentration. PubMed: 23451260

ターゲット情報

  • 機能

    Involved in adaptive response to hypoxia. Suppresses hypoxia-inducible expression of HIF1A and EPAS1. Binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. Isoform 4 has a dominant-negative function of inactivating HIF1A-mediated transcription. Isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secure an inhibition of HIF1A activity. Isoform 4 may be a negative regulator of hypoxia-inducible gene expression in the kidney and may be involved in renal tumorigenesis. Functions as an inhibitor of angiogenesis in the cornea.

  • 組織特異性

    Expressed in kidney. Expressed abundantly in lung epithelial cells. Expression is regulated in an oxygen-dependent manner.

  • 配列類似性

    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.

  • 翻訳後修飾

    In normoxia, hydroxylated on Pro-492 in the oxygen-dependent degradation domain (ODD) by PHD. The hydroxylated proline promotes interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation.

  • 細胞内局在

    Nucleus. Cytoplasm. In the nuclei of all periportal and perivenous hepatocytes. In the distal perivenous zone, detected in the cytoplasm of the hepatocytes.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Basic-helix-loop-helix-PAS protein MOP7 antibody
      • bHLHe17 antibody
      • Class E basic helix-loop-helix protein 17 antibody
      • HIF 3A antibody
      • HIF 3A4 antibody
      • HIF-3-alpha antibody
      • HIF3 alpha antibody
      • HIF3-alpha antibody
      • HIF3-alpha-1 antibody
      • HIF3A antibody
      • HIF3A_HUMAN antibody
      • Hypoxia Inducible Factor 3 alpha antibody
      • Hypoxia inducible factor 3 alpha subunit antibody
      • Hypoxia inducible factor three alpha antibody
      • Hypoxia-inducible factor 3-alpha antibody
      • Inhibitory PAS domain protein antibody
      • IPAS antibody
      • Member of PAS protein 7 antibody
      • MOP7 antibody
      • PAS domain-containing protein 7 antibody
      • PASD7 antibody
      see all

    画像

    • Western blot - Anti-HIF3 alpha/IPAS antibody (ab2165)
      Western blot - Anti-HIF3 alpha/IPAS antibody (ab2165)
      Anti-HIF3 alpha/IPAS antibody (ab2165) at 1/1600 dilution + 3T3 lysate treated with Cobalt (II) chloride at 10 µg/ml

      Secondary
      Goat-anti-Rabbit at 1/10000 dilution

      Predicted band size: 72 kDa
      Observed band size: 72 kDa
      Additional bands at: 24 kDa, 55 kDa. We are unsure as to the identity of these extra bands.



      Exponentially growing 3T3 cells were treated with 130µM CoCl2 for 6 hours at 37°C to mimic hypoxia. A clear band at ~ 72kDa is detected in this lysate although some weaker background staining is also seen.

    参考文献

    ab2165 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2165 は 2 報の論文で使用されています。

    • Huang Y  et al. Hypoxia inducible factor 3a plays a critical role in alveolarization and distal epithelial cell differentiation during mouse lung development. PLoS One 8:e57695 (2013). IHC-P ; Mouse . PubMed: 23451260
    • Huang C & Hales BF Effects of exposure to a DNA damaging agent on the hypoxia inducible factors in organogenesis stage mouse limbs. PLoS One 7:e51937 (2012). WB ; Mouse . PubMed: 23251655

    レビューと Q&A

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    Question

    Thanks for the antibody info! I think we will order it. I have one question: which isoform(s) does this antibody react with? Thanks!

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    Answer

    The antibody recognises Hif-3 alpha. The sequence of the immunogen is EIKPPKRSPRLE conjugated to KLH, corresponding to amino acids 581 - 592 of Mouse HIF-3 alpha.

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