Anti-HIF3 alpha/IPAS 抗体 (ab2165)

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Key features and details

  • Rabbit polyclonal to HIF3 alpha/IPAS
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-HIF3 alpha/IPAS antibody
    HIF3 alpha/IPAS 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to HIF3 alpha/IPAS

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Mouse
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide corresponding to Rabbit HIF3 alpha/IPAS aa 581-592 conjugated to keyhole limpet haemocyanin.
    Sequence:

    EIKPPKRSPRLE

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 特記事項

    Previously labelled as HIF3 alpha. 

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1600. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
IHC-P Use at an assay dependent concentration. PubMed: 23451260

ターゲット情報

  • 機能

    Involved in adaptive response to hypoxia. Suppresses hypoxia-inducible expression of HIF1A and EPAS1. Binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. Isoform 4 has a dominant-negative function of inactivating HIF1A-mediated transcription. Isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secure an inhibition of HIF1A activity. Isoform 4 may be a negative regulator of hypoxia-inducible gene expression in the kidney and may be involved in renal tumorigenesis. Functions as an inhibitor of angiogenesis in the cornea.

  • 組織特異性

    Expressed in kidney. Expressed abundantly in lung epithelial cells. Expression is regulated in an oxygen-dependent manner.

  • 配列類似性

    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.

  • 翻訳後修飾

    In normoxia, hydroxylated on Pro-492 in the oxygen-dependent degradation domain (ODD) by PHD. The hydroxylated proline promotes interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation.

  • 細胞内局在

    Nucleus. Cytoplasm. In the nuclei of all periportal and perivenous hepatocytes. In the distal perivenous zone, detected in the cytoplasm of the hepatocytes.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Basic-helix-loop-helix-PAS protein MOP7 antibody
      • bHLHe17 antibody
      • Class E basic helix-loop-helix protein 17 antibody
      • HIF 3A antibody
      • HIF 3A4 antibody
      • HIF-3-alpha antibody
      • HIF3 alpha antibody
      • HIF3-alpha antibody
      • HIF3-alpha-1 antibody
      • HIF3A antibody
      • HIF3A_HUMAN antibody
      • Hypoxia Inducible Factor 3 alpha antibody
      • Hypoxia inducible factor 3 alpha subunit antibody
      • Hypoxia inducible factor three alpha antibody
      • Hypoxia-inducible factor 3-alpha antibody
      • Inhibitory PAS domain protein antibody
      • IPAS antibody
      • Member of PAS protein 7 antibody
      • MOP7 antibody
      • PAS domain-containing protein 7 antibody
      • PASD7 antibody
      see all

    画像

    • Western blot - Anti-HIF3 alpha/IPAS antibody (ab2165)
      Western blot - Anti-HIF3 alpha/IPAS antibody (ab2165)
      Anti-HIF3 alpha/IPAS antibody (ab2165) at 1/1600 dilution + 3T3 lysate treated with Cobalt (II) chloride at 10 µg/ml

      Secondary
      Goat-anti-Rabbit at 1/10000 dilution

      Predicted band size: 72 kDa
      Observed band size: 72 kDa
      Additional bands at: 24 kDa, 55 kDa. We are unsure as to the identity of these extra bands.



      Exponentially growing 3T3 cells were treated with 130µM CoCl2 for 6 hours at 37°C to mimic hypoxia. A clear band at ~ 72kDa is detected in this lysate although some weaker background staining is also seen.

    参考文献 (3)

    ab2165 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2165 は 3 報の論文で使用されています。

    • Wang Y  et al. Berberine improves insulin resistance in adipocyte models by regulating the methylation of hypoxia-inducible factor-3a. Biosci Rep 39:N/A (2019). PubMed: 31652442
    • Huang Y  et al. Hypoxia inducible factor 3a plays a critical role in alveolarization and distal epithelial cell differentiation during mouse lung development. PLoS One 8:e57695 (2013). IHC-P ; Mouse . PubMed: 23451260
    • Huang C & Hales BF Effects of exposure to a DNA damaging agent on the hypoxia inducible factors in organogenesis stage mouse limbs. PLoS One 7:e51937 (2012). WB ; Mouse . PubMed: 23251655

    レビューと Q&A

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    Question

    Thanks for the antibody info! I think we will order it. I have one question: which isoform(s) does this antibody react with? Thanks!

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    Answer

    The antibody recognises Hif-3 alpha. The sequence of the immunogen is EIKPPKRSPRLE conjugated to KLH, corresponding to amino acids 581 - 592 of Mouse HIF-3 alpha.

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