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Our Abpromise guarantee covers the use of ab2165 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1600. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).|
|IHC-P||Use at an assay dependent concentration. PubMed: 23451260|
Exponentially growing 3T3 cells were treated with 130µM CoCl2 for 6 hours at 37°C to mimic hypoxia. A clear band at ~ 72kDa is detected in this lysate although some weaker background staining is also seen.
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