Application
Western blot
Sample
Rat Tissue lysate - whole (Kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1XTBS
Dilution
1/1000
Secondary antibody
Dilution
1/4000
Name
Non-Abcam antibody was used: Anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Chemi
Exposure
30 second(s)
Additional data
Additional Notes
lane 1,2,3= rat1(triplicate): control Kidney
lane 4,5,6= rat2(triplicate): Hypoxic Kidney (Chronic heart failure induced by myocardial-infarction)
Note: The detected band is of ~80kDa, not as expected for HIF2a (120kDa).
Abcam response
Thank you for submitting your feedback in the form of an Abreview. I am sorry that the antibody did not work as it should and will have the lab investigate this issue. We do guarantee the antibody for Western Blot and Rat under the Abpromise guarantee, and can provide a replacement or refund within 12 months from purchase.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jun 12 2019