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Anti-HIF-2-alpha 抗体 [BL-95-1A2] - BSA free (ab272041)

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ChIP-sequencing - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)
  • Immunocytochemistry - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)
  • Immunocytochemistry - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)
  • Immunoprecipitation - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)
  • Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BL-95-1A2] to HIF-2-alpha - BSA free
  • Suitable for: IP, ChIP-sequencing, ICC, IHC-P
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free
    HIF-2-alpha 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [BL-95-1A2] to HIF-2-alpha - BSA free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • 免疫原

    Synthetic peptide within Human HIF-2-alpha aa 400-450. The exact sequence is proprietary. NP_001421.2 and Gene ID 2034.
    Database link: Q99814

  • 特記事項

    This product is sold under License from Bethyl Laboratories, Inc.

    ab272041 is the carrier-free version of ab243861. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

関連製品

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272041 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
ICC
Human
IHC-P
Human
IP
Human
アプリケーション Abreviews 特記事項
IP
Use at an assay dependent concentration.
ChIP-sequencing
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
特記事項
IP
Use at an assay dependent concentration.
ChIP-sequencing
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.

  • 組織特異性

    Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.

  • 関連疾患

    Defects in EPAS1 are the cause of erythrocytosis familial type 4 (ECYT4) [MIM:611783]. ECYT4 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated hemoglobin concentration and hematocrit, and normal platelet and leukocyte counts.

  • 配列類似性

    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.

  • 翻訳後修飾

    In normoxia, is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization.
    In normoxia, is hydroxylated on Asn-847 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation.
    Phosphorylated on multiple sites in the CTAD.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Basic helix loop helix PAS protein MOP2 antibody
      • Basic-helix-loop-helix-PAS protein MOP2 antibody
      • bHLHe73 antibody
      • Class E basic helix-loop-helix protein 73 antibody
      • ECYT4 antibody
      • Endothelial PAS domain containing protein 1 antibody
      • Endothelial pas domain protein 1 antibody
      • Endothelial PAS domain-containing protein 1 antibody
      • EPAS 1 antibody
      • EPAS-1 antibody
      • EPAS1 antibody
      • EPAS1_HUMAN antibody
      • HIF 1 alpha like factor antibody
      • HIF 2 alpha antibody
      • HIF-1-alpha-like factor antibody
      • HIF-2-alpha antibody
      • HIF2-alpha antibody
      • HIF2A antibody
      • HLF antibody
      • Hypoxia inducible factor 2 alpha antibody
      • Hypoxia inducible factor 2 alpha subunit antibody
      • Hypoxia-inducible factor 2-alpha antibody
      • Member of PAS protein 2 antibody
      • Member of pas superfamily 2 antibody
      • MOP 2 antibody
      • MOP2 antibody
      • PAS domain-containing protein 2 antibody
      • PASD2 antibody
      see all

    画像

    • ChIP-sequencing - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)
      ChIP-sequencing - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)
      Localization of human HIF2-alpha binding sites in immunoprecipitates from subcutaneous tumor 786-O cells by ChIP-Seq using ab243861.

      This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243861).

    • Immunocytochemistry - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)
      Immunocytochemistry - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)
      Formaldehyde-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells untreated (Left) and treated with CoCl2 (right), labeling HIF-2 alpha (Red) using ab243861 at 1/100 dilution. DyLight® 594-conjugated goat anti-rabbit IgG was used as the secondary antibody at 1/100 dilution. Phalloidin Alexa Fluor® 488conjugated (green) was used as the counterstain.

      This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243861).

    • Immunocytochemistry - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)
      Immunocytochemistry - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)
      Formalin-fixed, paraffin-embedded HepG2 (human liver hepatocellular carcinoma cell line) cells labeling HIF-2 alpha using ab243861 at 1/400 dilution in ICC/IF analysis. A HRP-conjugated goat-anti rabiit IgG was used as the secondary. DAB staining.

      This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243861).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)
      Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for HIF-2 alpha using ab243861 at 1/400 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.

      This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243861).

    • Immunoprecipitation - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)
      Immunoprecipitation - Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)

      HIF-2 alpha was immunoprecipitated from 1 mg HepG2 whole cell lysate with ab243861 at 20 µL per reaction. Western blot was performed on the immunoprecipitate using ab243861 at 1/1000 dilution.

      Lane 1: ab243861 IP in HepG2 cell lysate treated with 200 µM CoCl2.

      Lane 2: ab243861 IP in HepG2 whole cell lysate Mock treated.

      Lane 3: Contol IgG in HepG2 cell lysate treated with 200 µM CoCl2.

      This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243861).

    • Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)
      Anti-HIF-2-alpha antibody [BL-95-1A2] - BSA free (ab272041)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab272041 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab272041 は論文での使用が確認できていません。

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