Anti-HIF-2-alpha 抗体 (ab199)
Key features and details
- Rabbit polyclonal to HIF-2-alpha
- Suitable for: IHC-P, WB
- Reacts with: Human, African green monkey
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-HIF-2-alpha antibody
HIF-2-alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HIF-2-alpha -
由来種
Rabbit -
特異性
Specific for HIF-2-alpha / EPAS 1. Does not cross react with HIF-1-alpha. -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human, African green monkey -
免疫原
Synthetic peptide corresponding to Mouse HIF-2-alpha aa 632-646.
Sequence:GRSNTQWPPDPPLHF
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ポジティブ･コントロール
- WB: Hypoxic HeLa lysate; COS7 nuclear extracts IHC: Human cardiac myocytes
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab199の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
1/100.
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| WB | (9) |
1/200 - 1/1000. Detects a band of approximately 118 kDa (predicted molecular weight: 100 kDa). The following should be taken into consideration: sufficient hypoxia induction is necessary; use nuclear extracts for cleaner results; use positive/negative controls to see which band is up-regulated; HIF2a degrades rapidly (fast sample preparation, protease inhibitors!).
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| 特記事項 |
|---|
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IHC-P
1/100. |
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WB
1/200 - 1/1000. Detects a band of approximately 118 kDa (predicted molecular weight: 100 kDa). The following should be taken into consideration: sufficient hypoxia induction is necessary; use nuclear extracts for cleaner results; use positive/negative controls to see which band is up-regulated; HIF2a degrades rapidly (fast sample preparation, protease inhibitors!). |
ターゲット情報
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機能
Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD. -
組織特異性
Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells. -
関連疾患
Defects in EPAS1 are the cause of erythrocytosis familial type 4 (ECYT4) [MIM:611783]. ECYT4 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated hemoglobin concentration and hematocrit, and normal platelet and leukocyte counts. -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains. -
翻訳後修飾
In normoxia, is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization.
In normoxia, is hydroxylated on Asn-847 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation.
Phosphorylated on multiple sites in the CTAD.
The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 2034 Human
- Omim: 603349 Human
- SwissProt: Q99814 Human
- Unigene: 468410 Human
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別名
- Basic helix loop helix PAS protein MOP2 antibody
- Basic-helix-loop-helix-PAS protein MOP2 antibody
- bHLHe73 antibody
see all
画像
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Western blot - Anti-HIF-2-alpha antibody (ab199)Anti-HIF-2-alpha antibody (ab199) + Hypoxic HeLa cell lysate at 0.5 mg/ml
Performed under reducing conditions.
Predicted band size: 100 kDaDetection of HIF-2-alpha in hypoxic HeLa lysate using ab199, reducing conditions using the 12-230 kDa separation system.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF-2-alpha antibody (ab199)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of huaman cardiac myocytes labeling HIF-2-alpha with ab199 at 1/100 dilution.
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Western blot - Anti-HIF-2-alpha antibody (ab199)All lanes : Anti-HIF-2-alpha antibody (ab199)
Lane 1 : Cobalt chloride treated COS7 nuclear extracts
Lane 2 : Untreated COS7 nuclear extracts
Predicted band size: 100 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (117)
ab199 は 117 報の論文で使用されています。
- Macias D et al. Targeting HIF2a-ARNT hetero-dimerisation as a novel therapeutic strategy for pulmonary arterial hypertension. Eur Respir J 57:N/A (2021). PubMed: 32972983
- Pantazopoulou V et al. Hypoxia-Induced Reactivity of Tumor-Associated Astrocytes Affects Glioma Cell Properties. Cells 10:N/A (2021). PubMed: 33802060
- Niklasson CU et al. Hypoxia inducible factor-2a importance for migration, proliferation, and self-renewal of trunk neural crest cells. Dev Dyn 250:191-236 (2021). PubMed: 32940375
- Chan CD et al. Co-localisation of intra-nuclear membrane type-1 matrix metalloproteinase and hypoxia inducible factor-2a in osteosarcoma and prostate carcinoma cells. Oncol Lett 21:158 (2021). PubMed: 33552276
- Lyu Q et al. Intermittent short-duration reoxygenation protects against simulated high altitude-induced pulmonary hypertension in rats. FASEB J 35:e21212 (2021). PubMed: 33230951