Application
Western blot
Sample
Mouse Cell lysate - nuclear (NMuMG cells)
Loading amount
25 µg
Specification
NMuMG cells
Treatment
Check comments
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Milk 1% in TBS T
Secondary antibody
Name
Non-Abcam antibody was used: DAKO anti mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ecl
Exposure
20 minute(s) and 0 second(s)
Bands
Specific: 60 kDa
Positive control
Cells under Hipoxia conditions that promotes HIF 1a expression
Additional data
PubMed ID
19955572: View
Additional Notes
MCF-7 cells were cultured under normoxic (N) or hypoxic(H) conditions for72h or in normoxic condition withTGF-b1(5 ng/ml) for the same length of time.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jan 21 2013