Application
Western blot
Sample
Cat Cell lysate - other (cells)
Loading amount
50 µg
Specification
cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: pbst
Secondary antibody
Name
Non-Abcam antibody was used: secondary antibody
Host species: Branchiostoma belcheri
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Host species: Branchiostoma belcheri
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Dilution
1/5000
Detection
Detection method
ecl
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 123 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 18 2013