Anti-HIF-1 alpha 抗体 [H1alpha67] (ab1)
Key features and details
- Mouse monoclonal [H1alpha67] to HIF-1 alpha
- Suitable for: IP, Flow Cyt, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-HIF-1 alpha antibody [H1alpha67]
HIF-1 alpha 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [H1alpha67] to HIF-1 alpha -
由来種
Mouse -
アプリケーション
適用あり: IP, Flow Cyt, ICC/IF, WBmore details
適用なし: IHC-Fr or IHC-P -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Fusion protein corresponding to Human HIF-1 alpha aa 400-550.
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ポジティブ・コントロール
- WB: HeLa (DFO treated 0.5mM, 24h) nuclear lysate (ab180880); Human whole cell lysate (human lung adenocarcinoma cell line ADLC-5M2) (DFO treated 100uM, 16h). IP: HeLa (DFO treated 0.5 mg) nuclear lysate. ICC: MCF7 cells. Flow Cyt: HeLa (Human epithelial cell line from cervix adenocarcinoma) cells treated with 1mM Deferoxamine (ab120727) for 24 hours.
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特記事項
For WB, we recommend using positive control samples such as DFO or CoCl2 treated nulcear cell lysates such as ab180880. Ensure cell lysis occurs quickly (within 2 mins) if removed from hypoxia. Loading a high amount of sample (>50 µg) and addition of protease inhibitors (e.g. ab65621) may also enhance detection.
This antibody clone is manufactured by Abcam. If you require a custom formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
H1alpha67 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Alternative Versions
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Assay kits
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
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- HeLa nuclear extract lysate (ab150036)
- Human HIF-1 alpha ELISA Kit (ab171577)
- HIF-1 alpha (H1alpha67, EP1215Y, ESEE122, EPR16897) Antibody Panel (ab263460)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab1の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP | (2) |
Use a concentration of 5 µg/ml.
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Flow Cyt |
Use at an assay dependent concentration.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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ICC/IF | (6) |
1/100 - 1/200.
PubMed: 25422886 We recommend Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879) secondary antibody. |
WB | (22) |
Use a concentration of 5 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 92 kDa).
We recommend blocking for 1 hour with 5% milk in TBST and reducing to 2% milk in TBST for the primary and secondary antibody incubation steps. For primary antibody incubation, we recommend 2 hours at room temperature. We recommend Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) secondary antibody.
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特記事項 |
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IP
Use a concentration of 5 µg/ml. |
Flow Cyt
Use at an assay dependent concentration. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/100 - 1/200. PubMed: 25422886 We recommend Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879) secondary antibody. |
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 92 kDa). We recommend blocking for 1 hour with 5% milk in TBST and reducing to 2% milk in TBST for the primary and secondary antibody incubation steps. For primary antibody incubation, we recommend 2 hours at room temperature. We recommend Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) secondary antibody.
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ターゲット情報
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機能
Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions activates the transcription of over 40 genes, including, erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. -
組織特異性
Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains. -
ドメイン
Contains two independent C-terminal transactivation domains, NTAD and CTAD, which function synergistically. Their transcriptional activity is repressed by an intervening inhibitory domain (ID). -
翻訳後修飾
In normoxia, is hydroxylated on Pro-402 and Pro-564 in the oxygen-dependent degradation domain (ODD) by EGLN1/PHD1 and EGLN2/PHD2. EGLN3/PHD3 has also been shown to hydroxylate Pro-564. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Deubiquitinated by USP20. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization.
In normoxia, is hydroxylated on Asn-803 by HIF1AN, thus abrogating interaction with CREBBP and EP300 and preventing transcriptional activation. This hydroxylation is inhibited by the Cu/Zn-chelator, Clioquinol.
S-nitrosylation of Cys-800 may be responsible for increased recruitment of p300 coactivator necessary for transcriptional activity of HIF-1 complex.
Requires phosphorylation for DNA-binding.
Sumoylated; by SUMO1 under hypoxia. Sumoylation is enhanced through interaction with RWDD3. Desumoylation by SENP1 leads to increased HIF1A stability and transriptional activity.
Ubiquitinated; in normoxia, following hydroxylation and interaction with VHL. Lys-532 appears to be the principal site of ubiquitination. Clioquinol, the Cu/Zn-chelator, inhibits ubiquitination through preventing hydroxylation at Asn-803.
The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains. -
細胞内局在
Cytoplasm. Nucleus. Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. Colocalizes with SUMO1 in the nucleus, under hypoxia. - Information by UniProt
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参照データベース
- Entrez Gene: 3091 Human
- Entrez Gene: 15251 Mouse
- Entrez Gene: 29560 Rat
- Omim: 603348 Human
- SwissProt: Q16665 Human
- SwissProt: Q61221 Mouse
- SwissProt: O35800 Rat
- Unigene: 597216 Human
see all -
別名
- ARNT interacting protein antibody
- ARNT-interacting protein antibody
- Basic helix loop helix PAS protein MOP1 antibody
see all
画像
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All lanes : Anti-HIF-1 alpha antibody [H1alpha67] (ab1) at 5 µg/ml
Lane 1 :HeLa nuclear extract lysate (ab150036) at 40 µg
Lane 2 :Hela-DFO treated (0.5mM, 24h) Nuclear Lysate (ab180880) at 40 µg
Lane 3 : HeLa nuclear control at 40 µg
Lane 4 : HeLa nuclear DFO treated at 40 µg
Lane 5 :Recombinant Human HIF-1 alpha protein (ab154478) at 0.001 µg
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 92 kDa
Exposure time: 20 minutesWe recommend using 5% milk in TBST as the blocking agent, decreasing to 2% milk in TBST during primary and secondary antibody incubation.
Blots were developed with Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) secondary antibody
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HIF-1 alpha was immunoprecipitated using 0.5 mg HeLa Nuclear DFO treated whole cell extract (ab180880), 5 µg of Mouse monoclonal to HIF-1 alpha and 50 µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10 minutes, HeLa DFO treated whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10 minutes under agitation.
Proteins were eluted by addition of 40 µl SDS loading buffer and incubated for 10 minutes at 70°C; 10 µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab1.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1:20,000 dilution.
Band: 110 kDa; HIF1 alpha
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Western blot - Anti-HIF-1 alpha antibody [H1alpha67] (ab1)This image is taken from an Abreview submitted by Mike Campa, no further information is known about this imageAnti-HIF-1 alpha antibody [H1alpha67] (ab1) at 1/400 dilution + Human whole cell lysate (human lung adenocarcinoma cell line ADLC-5M2) treated for 16 hours with 100 micromolar deferoxamine (DFO) at 20 µg
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
PVDF membrane was used and blocked for 16 hours in 5% milk. -
ab1 staining HIF-1-alpha in MCF7 (Human breast adenocarcinoma cell line) cells treated with metformin hydrochloride ab12084, by ICC/IF. Decrease in HIF-1-alpha expression correlates with increased concentration of metformin hydrochloride, as described in literature.
The cells were incubated at 37°C for 24 hours in media containing different concentrations of ab120847 (metformin hydrochloride) in water, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2 hours at room temperature. Staining of the treated cells with ab1 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue. -
Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody [H1alpha67] (ab1)This Image is courtesy of an anonymous Abreview
ab1 at 1/200 dilution staining HIF-1 alpha in human 293FT cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde, permeabilized in 0.5% Trition X-100 and blocked in 5% BSA for 1 hour at 25°C. The primary antibody was used at 1/200 dilution in PBS and incubated with sample at 4°C for 12 hours. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG was used as secondary at 1/500 dilution.
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Flow cytometry using ab1. HeLa (Human epithelial cell line from cervix adenocarcinoma) cells were cultured untreated or with 1mM Deferoxamine (ab120727) for 24 hours to induce HIF-1-alpha protein levels. Cells were then trypsinized, fixed with paraformaldehyde and stained with ab1 (0.5 µg/mL). 1% BSA in PBS was used as the blocking buffer throughout. ab1 was labeled with and anti-mouse Alexa-Fluor® 488 dye. Unstained (black), untreated (red) and DFO treated (blue) cell traces are shown.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (240)
ab1 は 240 報の論文で使用されています。
- Lai HT et al. GATA6 enhances the stemness of human colon cancer cells by creating a metabolic symbiosis through upregulating LRH-1 expression. Mol Oncol 14:1327-1347 (2020). PubMed: 32037723
- Wang J et al. Inhibition of PLK4 might enhance the anti-tumour effect of bortezomib on glioblastoma via PTEN/PI3K/AKT/mTOR signalling pathway. J Cell Mol Med 24:3931-3947 (2020). PubMed: 32126150
- Zhou Y et al. HIF1A activates the transcription of lncRNA RAET1K to modulate hypoxia-induced glycolysis in hepatocellular carcinoma cells via miR-100-5p. Cell Death Dis 11:176 (2020). PubMed: 32152275
- Zhang G et al. HIF-1a/microRNA-128-3p axis protects hippocampal neurons from apoptosis via the Axin1-mediated Wnt/ß-catenin signaling pathway in Parkinson's disease models. Aging (Albany NY) 12:4067-4081 (2020). PubMed: 32167488
- Guo Y et al. DNA N6-methyladenine modification in hypertension. Aging (Albany NY) 12:6276-6291 (2020). PubMed: 32283543