Anti-HHV8 抗体 [LN53] (ab4103)

Submit a review Q&A (5)References (5)

Key features and details

  • Rat monoclonal [LN53] to HHV8
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Species independent
  • Isotype: IgG2c

製品の概要

  • 製品名

    Anti-HHV8 antibody [LN53]
    HHV8 一次抗体 製品一覧

  • 製品の詳細

    Rat monoclonal [LN53] to HHV8

  • 由来種

    Rat

  • 特異性

    HHV 8 is the likely etiological agent of Kaposi’s sarcoma (KS). HHV 8 encodes a latent nuclear antigen (LNA), which is the product of the viral gene orf 73. LNA is capable of forming a complex with retinoblastoma susceptibility gene product, which may be related to its oncogenic activity.

  • アプリケーション

    適用あり: IHC-P, IHC-Frmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    Recombinant protein corresponding to the latent nuclear antigen 1 molecule of HHV 8.

  • エピトープ

    Located within the ORF-73 region.

  • ポジティブ･コントロール

    • Kaposi’s sarcoma.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.05% Sodium azide
    Constituents: Tissue culture supernatant, 1% BSA
  • Concentration information loading...

  • 精製度

    IgG fraction

  • ポリ/モノ

    モノクローナル

  • クローン名

    LN53

  • アイソタイプ

    IgG2c

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab4103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC method.
IHC-Fr 1/25 - 1/50. ABC method.

ターゲット情報

  • 関連性

    HHV8 has been found to be associated with three different diseases observed in AIDS patients; kaposi's sarcoma, primary effusion lymphoma (which is a rare type of non-Hodgkin lymphoma affecting the body cavities) and multicentric Castleman's disease. To date there is much evidence to support a direct role for HHV8 in kaposi's sarcoma.

  • 別名

    • HHV 8 antibody
    • Human Herpes Virus 8 antibody
    • Human herpesvirus 8 antibody
    • Kaposi's sarcoma associated herpes virus antibody
    • KSHV antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HHV8 antibody [LN53] (ab4103)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HHV8 antibody [LN53] (ab4103)
    Cultured cells infected with HHV8, stained with HHV8 using ABC and DAB chromogen.

参考文献 (5)

ab4103 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab4103 は 5 報の論文で使用されています。

  • Tan B  et al. Viral and cellular N6-methyladenosine and N6,2'-O-dimethyladenosine epitranscriptomes in the KSHV life cycle. Nat Microbiol 3:108-120 (2018). PubMed: 29109479
  • Yang J  et al. Case report of ocular Kaposi's sarcoma. BMC Ophthalmol 17:143 (2017). PubMed: 28806949
  • Zhu Y  et al. An Oncogenic Virus Promotes Cell Survival and Cellular Transformation by Suppressing Glycolysis. PLoS Pathog 12:e1005648 (2016). IHC-P . PubMed: 27187079
  • El Hajj H  et al. Combination of arsenic and interferon-a inhibits expression of KSHV latent transcripts and synergistically improves survival of mice with primary effusion lymphomas. PLoS One 8:e79474 (2013). ICC/IF . PubMed: 24250827
  • Loddenkemper C  et al. [The morphologic variants of KSHV/HHV 8-associated lymphoproliferations.] Pathologe : (2006). PubMed: 17160657

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Question


I would like to know which region of LANA this antibody recognizes, N-terminal, C-terminal or central repeat region?

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Answer


The specific epitope of ab4103 is near the C terminus of the ORF-73 region within HHV8. Below is more information about specific recombinant proteins that this antibody recognizes.

GST-C14, GST-C17, GST-C7, and TH-C14;

GST-C14 contains amino acids 803 to 1113 of orf73 encompassing 126 amino acids of the central repeat domain as well as 184 amino acids of the C-terminal basic domain.

GST-C17 contains amino acids 803 to 942 comprised almost entirely of repetitive coding sequence.

GST-C14 served as the backbone for making further defined deletions of the C terminus of orf73 by using three unique restriction enzyme sites.

GST-C7 was constructed by digesting GST-C14 with NruI (in orf73) and NotI (in pGEX-4T3 [Pharmacia]), followed by end repair and ligation.

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Question

A happy new year to you and all at Abcam.

We have an inquiry for the HHV8 - LANA1 antibody. The customer has used Leica before. Kindly advise, from the many different HHV8 antibodies that Abcam have, which one matches the Leica HHV8-LNA, Clone 13B10, so we can quote.

Best regards

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Answer

Thank you for contacting us.

We do not have clone 13B10 in catalogue like the Leica microsystem. We however have antibody that is specific to HHV8- latent nuclear antigen that can quoted to customer;

https://www.abcam.com/hhv8-antibody-ln35-ab4103.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Customer contacted us regarding location of epitope on HHV8 sequence.

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Answer



The laboratory responded that the epitope for ab4103 is near the C terminus of ORF 73 within HHV8.

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Question

Please provide list of antibodies to HHV8 virus

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Answer

Thank you for your telephone enquiry yesterday afternoon. As requested, here is a list of all our antibodies for detection of HHV8 virus. I can recommend to review the online datasheets for further information. ab4103 Rat monoclonal Anti-HHV8 antibody [LN35] Applications: IHC-Fr, IHC-P Click here (or use the following: https://www.abcam.com/index.html?datasheet=4103). ab12208 Rat monoclonal Anti-HHV8 Cyclin antibody [94B] Applications: IF, IP, WB Click here (or use the following: https://www.abcam.com/index.html?datasheet=12208). ab25122 Rabbit polyclonal Anti-HHV8 GPCR antibody Applications: Flow Cyt Click here (or use the following: https://www.abcam.com/index.html?datasheet=25122). ab16159 Sheep polyclonal Anti-HHV8 ORF62 antibody Applications: WB Click here (or use the following: https://www.abcam.com/index.html?datasheet=16159). I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Question

has this antibody cross-reactivity with mouse antibody in a double staining immunofluorescence?

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Answer

Thank you for your enquiry and your patience in awaiting a response. HHV8 is human herpes virus and was tested only on human infected tissue. It was not tested for any other virus infected animal tissues. If you have any further questions then please do not hesitate to get back in touch with us.

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