Anti-HHV8 抗体 [LN53] (ab4103)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HHV8 antibody [LN53] (ab4103)

    Key features and details

    • Rat monoclonal [LN53] to HHV8
    • Suitable for: IHC-P, IHC-Fr
    • Reacts with: Species independent
    • Isotype: IgG2c

    製品の概要

    • 製品名

      Anti-HHV8 antibody [LN53]
      HHV8 一次抗体 製品一覧

    • 製品の詳細

      Rat monoclonal [LN53] to HHV8

    • 由来種

      Rat

    • 特異性

      HHV 8 is the likely etiological agent of Kaposi’s sarcoma (KS). HHV 8 encodes a latent nuclear antigen (LNA), which is the product of the viral gene orf 73. LNA is capable of forming a complex with retinoblastoma susceptibility gene product, which may be related to its oncogenic activity.

    • アプリケーション

      適用あり: IHC-P, IHC-Frmore details

    • 種交差性

      交差種: Species independent

    • 免疫原

      Recombinant full length protein corresponding to HHV8. Corresponding to the latent nuclear antigen 1 molecule of HHV 8.

    • エピトープ

      Located within the ORF-73 region.

    • ポジティブ･コントロール

      • Kaposi’s sarcoma.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • バッファー

      pH: 7.3
      Preservative: 0.05% Sodium azide
      Constituents: Tissue culture supernatant, 1% BSA
    • Concentration information loading...

    • 精製度

      IgG fraction

    • ポリ/モノ

      モノクローナル

    • クローン名

      LN53

    • アイソタイプ

      IgG2c

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab4103の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P
    1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC method.
    IHC-Fr
    1/25 - 1/50. ABC method.
    特記事項
    IHC-P
    1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC method.
    IHC-Fr
    1/25 - 1/50. ABC method.

    ターゲット情報

    • 関連性

      HHV8 has been found to be associated with three different diseases observed in AIDS patients; kaposi's sarcoma, primary effusion lymphoma (which is a rare type of non-Hodgkin lymphoma affecting the body cavities) and multicentric Castleman's disease. To date there is much evidence to support a direct role for HHV8 in kaposi's sarcoma.

    • 別名

      • HHV 8 antibody
      • Human Herpes Virus 8 antibody
      • Human herpesvirus 8 antibody
      • Kaposi's sarcoma associated herpes virus antibody
      • KSHV antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HHV8 antibody [LN53] (ab4103)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HHV8 antibody [LN53] (ab4103)
      Cultured cells infected with HHV8, stained with HHV8 using ABC and DAB chromogen.

    参考文献 (9)

    ab4103 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab4103 は 9 報の論文で使用されています。

    • Vladimirova O  et al. Phase separation and DAXX redistribution contribute to LANA nuclear body and KSHV genome dynamics during latency and reactivation. PLoS Pathog 17:e1009231 (2021). PubMed: 33471863
    • Elbasani E  et al. Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication Is Independent of Anaphase-Promoting Complex Activity. J Virol 94:N/A (2020). PubMed: 32295923
    • Gramolelli S  et al. Oncogenic Herpesvirus Engages Endothelial Transcription Factors SOX18 and PROX1 to Increase Viral Genome Copies and Virus Production. Cancer Res 80:3116-3129 (2020). PubMed: 32518203
    • Naipauer J  et al. PDGFRA defines the mesenchymal stem cell Kaposi's sarcoma progenitors by enabling KSHV oncogenesis in an angiogenic environment. PLoS Pathog 15:e1008221 (2019). PubMed: 31881074
    • Tan B  et al. Viral and cellular N6-methyladenosine and N6,2'-O-dimethyladenosine epitranscriptomes in the KSHV life cycle. Nat Microbiol 3:108-120 (2018). PubMed: 29109479
    • Yang J  et al. Case report of ocular Kaposi's sarcoma. BMC Ophthalmol 17:143 (2017). PubMed: 28806949
    • Zhu Y  et al. An Oncogenic Virus Promotes Cell Survival and Cellular Transformation by Suppressing Glycolysis. PLoS Pathog 12:e1005648 (2016). IHC-P . PubMed: 27187079
    • El Hajj H  et al. Combination of arsenic and interferon-a inhibits expression of KSHV latent transcripts and synergistically improves survival of mice with primary effusion lymphomas. PLoS One 8:e79474 (2013). ICC/IF . PubMed: 24250827
    • Loddenkemper C  et al. [The morphologic variants of KSHV/HHV 8-associated lymphoproliferations.] Pathologe : (2006). PubMed: 17160657

    レビューと Q&A

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    1-5 of 5 Abreviews or Q&A

    Question


    I would like to know which region of LANA this antibody recognizes, N-terminal, C-terminal or central repeat region?

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    Answer


    The specific epitope of ab4103 is near the C terminus of the ORF-73 region within HHV8. Below is more information about specific recombinant proteins that this antibody recognizes.

    GST-C14, GST-C17, GST-C7, and TH-C14;

    GST-C14 contains amino acids 803 to 1113 of orf73 encompassing 126 amino acids of the central repeat domain as well as 184 amino acids of the C-terminal basic domain.

    GST-C17 contains amino acids 803 to 942 comprised almost entirely of repetitive coding sequence.

    GST-C14 served as the backbone for making further defined deletions of the C terminus of orf73 by using three unique restriction enzyme sites.

    GST-C7 was constructed by digesting GST-C14 with NruI (in orf73) and NotI (in pGEX-4T3 [Pharmacia]), followed by end repair and ligation.

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    Question

    A happy new year to you and all at Abcam.

    We have an inquiry for the HHV8 - LANA1 antibody. The customer has used Leica before. Kindly advise, from the many different HHV8 antibodies that Abcam have, which one matches the Leica HHV8-LNA, Clone 13B10, so we can quote.

    Best regards

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    Answer

    Thank you for contacting us.

    We do not have clone 13B10 in catalogue like the Leica microsystem. We however have antibody that is specific to HHV8- latent nuclear antigen that can quoted to customer;

    https://www.abcam.com/hhv8-antibody-ln35-ab4103.html

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question

    Customer contacted us regarding location of epitope on HHV8 sequence.

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    Answer



    The laboratory responded that the epitope for ab4103 is near the C terminus of ORF 73 within HHV8.

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    Question

    Please provide list of antibodies to HHV8 virus

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    Answer

    Thank you for your telephone enquiry yesterday afternoon. As requested, here is a list of all our antibodies for detection of HHV8 virus. I can recommend to review the online datasheets for further information. ab4103 Rat monoclonal Anti-HHV8 antibody [LN35] Applications: IHC-Fr, IHC-P Click here (or use the following: https://www.abcam.com/HHV8-antibody-LN53-ab4103.html). ab12208 Rat monoclonal Anti-HHV8 Cyclin antibody [94B] Applications: IF, IP, WB Click here (or use the following: https://www.abcam.com/v-cyclin-antibody-94B-ab12208.html). ab25122 Rabbit polyclonal Anti-HHV8 GPCR antibody Applications: Flow Cyt Click here (or use the following: https://www.abcam.com/vGPCR-antibody-ab25122.html). ab16159 Sheep polyclonal Anti-HHV8 ORF62 antibody Applications: WB Click here (or use the following: https://www.abcam.com/HHV8-ORF62-antibody-ab16159.html). I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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    Question

    has this antibody cross-reactivity with mouse antibody in a double staining immunofluorescence?

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    Answer

    Thank you for your enquiry and your patience in awaiting a response. HHV8 is human herpes virus and was tested only on human infected tissue. It was not tested for any other virus infected animal tissues. If you have any further questions then please do not hesitate to get back in touch with us.

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