Anti-HHV6AgQ1 抗体 [119] - BSA and Azide free (ab179902)
Key features and details
- Mouse monoclonal [119] to HHV6AgQ1
- Suitable for: Flow Cyt (Intra), ELISA, WB, ICC/IF, IP
- Reacts with: Human herpesvirus 6
- Isotype: IgG
製品の概要
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製品名
Anti-HHV6AgQ1 antibody [119] - BSA and Azide free -
製品の詳細
Mouse monoclonal [119] to HHV6AgQ1 -
由来種
Mouse -
特異性
ab179902 reacts with gQ1 of HHV6A and HHV6B. This antibody is not recommended for IP of HHV-6B. -
アプリケーション
適用あり: Flow Cyt (Intra), ELISA, WB, ICC/IF, IPmore details -
種交差性
交差種: Human herpesvirus 6 -
免疫原
Recombinant fragment (His-tag) corresponding to Human Herpesvirus HHV6AgQ1 aa 3-422. (Expressed in E. coli).
Sequence:TARLSAMKPPRSCALIFLCAFSMATAPTNATAHRRAGTVKSTPPPEDKHS YTAKYYDKDIYFNIYEGRNSTPRRRTLSEIISKFSTSEMLSLKRVKAFVP VDENPTTTLEDIADILNYAVCDDNSCGCTIETQARIMFGDIIICVPLSAD NKGVRNFKDRIMPKGLSQILSSSLGLHLSLLYGAFGSNYNSLAYMRRLKP LTAMTAIRFCPMTTKLELRQNYKVKETLCELIVSIEILKIRNNGGQTMKT LTSFAIVRKDNDGQDWETCTRFAPVNIEDILRYKRVANDTCCRHRDVQHG RRTLESSNSWTQTQYFEPWQDIVDVYVPINDTHCPNDSYVVFETLQGFEW CSRLNKNETKNYLSSVLGFRNALFETEELMETIAMRLASQILSMVGQQGT TIRDIDPAIVSALWHSLPEN
Database link: Q69572 -
ポジティブ・コントロール
- T-cell line HSB-2 cells infected with HHV-6A; 293T cells transfected with HHV6 U100 expression plasmid. 293T cells : Flow Cyt (Intra).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS -
Concentration information loading...
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精製度
Proprietary Purification -
特記事項(精製)
ab179902 is produced in serum-free medium and purified by proprietary chromatography procedures under mild conditions. ab179902 is 90~95% pure by SDS-PAGE and is filter-sterilised. -
ポリ/モノ
モノクローナル -
クローン名
119 -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab179902の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/100.
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ELISA |
Use at an assay dependent concentration.
assay dependent |
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WB |
1/500 - 1/1000.
|
|
ICC/IF |
1/100 - 1/3200.
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|
IP |
Use at an assay dependent concentration.
assay dependent |
特記事項 |
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Flow Cyt (Intra)
1/100. |
ELISA
Use at an assay dependent concentration. assay dependent |
WB
1/500 - 1/1000. |
ICC/IF
1/100 - 1/3200. |
IP
Use at an assay dependent concentration. assay dependent |
ターゲット情報
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関連性
Human herpesvirus 6 (HHV-6) is the common collective name for Human herpesvirus 6A (HHV-6A) and Human herpesvirus 6B (HHV-6B). These closely related viruses are two of the nine herpesviruses known to have humans as their primary host. HHV-6A and HHV-6B are double stranded DNA viruses within the betaherpesvirinae subfamily and of the genus Roseolovirus. HHV-6A and HHV-6B infects almost all of the human populations tested. The overall nucleotide sequence identity between HHV-6A and HHV-6B is 90% and they are now classified as distinct species. HHV-6A has been described as more neurovirulent, and as such is more frequently found in patients with neuroinflammatory diseases such as multiple sclerosis. HHV-6B primary infection is the cause of the common childhood illness exanthem subitum (also known as roseola infantum or sixth disease). Additionally, HHV-6B reactivation is common in transplant recipients, which can cause several clinical manifestations such as encephalitis, bone marrow suppression and pneumonitis. gQ1 encoded by the U100 gene of HHV6 is glycoprotein, complexes with gH, gL and gQ2 to form HHV6A ligand to CD46 receptor and HHV6B ligand to CD134 receptor. It is expressed in two different forms: an 80-kDa form (gQ1-80K) and a 74-kDa form (gQ1-74K) - only gQ1-80K, but not gQ1-74K, forms the ligand complex with gQ2, gH, and gL. It associates with lipid rafts.
画像
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All lanes : Anti-HHV6AgQ1 antibody [119] - BSA and Azide free (ab179902)
Lane 1 : Non-treated
Lane 2 : Treated with end-glycosilase
Lane 3 : Treated with peptide N -glycosidasegQ1 is detected as two glycoproteins with 80 kDa and 74 kDa molecular masses
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All lanes : Anti-HHV6AgQ1 antibody [119] - BSA and Azide free (ab179902)
Lane 1 : HSB-2 cells
Lane 2 : HSB-2 cells infected with HHV-6A GS strain -
Immunofluorescent analysis of HHV6A-infected HSB-2 cells labeling HHV6 U100 using FITC-conjugated ab179902 at 1/200 dilution. The infected cells were harvested 3 days postinfection, fixed in cold acetone.
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293T cells were transfected with gQ1 expression plasmid. The cells were harvested on second day postinfection, fixed with 4% PFA, permeabilized with 0.1% Triton-X100, incubated with anti-gQ1 antibody (119) and then with FITC-conjugated anti-mouse IgG antibody.Histograms show fluorescence intensities measured in arbitary units on a log scale (x axis) and relative cell numbers on a linear scale (y axis).Black line is control and red line is gQ1 introduced cells.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab179902 は 7 報の論文で使用されています。
- Tang H et al. Human herpesvirus 6 glycoprotein complex formation is required for folding and trafficking of the gH/gL/gQ1/gQ2 complex and its cellular receptor binding. J Virol 85:11121-30 (2011). PubMed: 21849437
- Tang H et al. Human herpesvirus 6 encoded glycoprotein Q1 gene is essential for virus growth. Virology 407:360-7 (2010). PubMed: 20863544
- Huang H et al. Human herpesvirus 6 envelope cholesterol is required for virus entry. J Gen Virol 87:277-85 (2006). PubMed: 16432012
- Mori Y et al. Discovery of a second form of tripartite complex containing gH-gL of human herpesvirus 6 and observations on CD46. J Virol 78:4609-16 (2004). PubMed: 15078943
- Akkapaiboon P et al. Intracellular processing of human herpesvirus 6 glycoproteins Q1 and Q2 into tetrameric complexes expressed on the viral envelope. J Virol 78:7969-83 (2004). PubMed: 15254169
- Mori Y et al. The human herpesvirus 6 U100 gene product is the third component of the gH-gL glycoprotein complex on the viral envelope. J Virol 77:2452-8 (2003). PubMed: 12551983
- Mori Y et al. Human herpesvirus 6 variant A glycoprotein H-glycoprotein L-glycoprotein Q complex associates with human CD46. J Virol 77:4992-9 (2003). PubMed: 12663806