Anti-HGS 抗体 - C-terminal (ab155539)
Key features and details
- Rabbit polyclonal to HGS - C-terminal
- Suitable for: IP, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-HGS antibody - C-terminal
HGS 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HGS - C-terminal -
由来種
Rabbit -
アプリケーション
適用あり: IP, WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment, corresponding to a region within C terminal amino acids 611-777 of Human HGS (UniProt ID: O14964).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab155539の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP | (1) |
Use at an assay dependent concentration.
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 86 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF | (1) |
1/100 - 1/1000.
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特記事項 |
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IP
Use at an assay dependent concentration. |
WB
1/1000 - 1/10000. Predicted molecular weight: 86 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation. -
組織特異性
Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes. -
配列類似性
Contains 1 FYVE-type zinc finger.
Contains 1 UIM (ubiquitin-interacting motif) repeat.
Contains 1 VHS domain. -
ドメイン
Has a double-sided UIM that can bind 2 ubiquitin molecules, one on each side of the helix.
The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions. -
翻訳後修飾
Phosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected (By similarity). Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF.
Ubiquitinated by ITCH. -
細胞内局在
Cytoplasm. Early endosome membrane. Endosome > multivesicular body membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 9146 Human
- Entrez Gene: 15239 Mouse
- Entrez Gene: 56084 Rat
- Omim: 604375 Human
- SwissProt: O14964 Human
- SwissProt: Q99LI8 Mouse
- SwissProt: Q9JJ50 Rat
- Unigene: 514590 Human
see all -
別名
- Hepatocyte growth factor regulated tyrosine kinase substrate antibody
- Hepatocyte growth factor-regulated tyrosine kinase substrate antibody
- HGF-regulated tyrosine kinase substrate antibody
see all
画像
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All lanes : Anti-HGS antibody - C-terminal (ab155539) at 1/10000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 86 kDa
7.5% SDS PAGE -
Anti-HGS antibody - C-terminal (ab155539) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 86 kDa
7.5% SDS PAGE -
Anti-HGS antibody - C-terminal (ab155539) at 1/1000 dilution + Rat brain whole cell lysate at 50 µg
Predicted band size: 86 kDa
7.5 % SDS-PAGE -
Confocal immunofluorescence analysis of methanol-fixed A431 cells, labeling HGS with ab155539 at 1/200 dilution (green) and Alpha-tubulin filaments (red).
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Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue, labeling HGS with ab155539 at 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab155539 は 8 報の論文で使用されています。
- Read CB et al. The Obligate Intracellular Bacterial Pathogen Anaplasma phagocytophilum Exploits Host Cell Multivesicular Body Biogenesis for Proliferation and Dissemination. mBio 13:e0296122 (2022). PubMed: 36409075
- Kim YH et al. Secretory autophagy machinery and vesicular trafficking are involved in HMGB1 secretion. Autophagy 17:2345-2362 (2021). PubMed: 33017561
- Coudert L et al. The ESCRT-0 subcomplex component Hrs/Hgs is a master regulator of myogenesis via modulation of signaling and degradation pathways. BMC Biol 19:153 (2021). PubMed: 34330273
- Lin H et al. miR-4454 Promotes Hepatic Carcinoma Progression by Targeting Vps4A and Rab27A. Oxid Med Cell Longev 2021:9230435 (2021). PubMed: 34777698
- Yu Z et al. Hepatocyte growth factor-regulated tyrosine kinase substrate is essential for endothelial cell polarity and cerebrovascular stability. Cardiovasc Res 117:533-546 (2021). PubMed: 32044971
- Cardoso RS et al. Host Retromer Protein Sorting Nexin 2 Interacts with Human Respiratory Syncytial Virus Structural Proteins and is Required for Efficient Viral Production. mBio 11:N/A (2020). PubMed: 32994321
- Tavares LA et al. Two Functional Variants of AP-1 Complexes Composed of either ?2 or ?1 Subunits Are Independently Required for Major Histocompatibility Complex Class I Downregulation by HIV-1 Nef. J Virol 94:N/A (2020). PubMed: 31915283
- Hammerling BC et al. A Rab5 endosomal pathway mediates Parkin-dependent mitochondrial clearance. Nat Commun 8:14050 (2017). PubMed: 28134239