Anti-HGS 抗体 (ab72053)
Key features and details
- Rabbit polyclonal to HGS
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-HGS antibody
HGS 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HGS -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Cow -
免疫原
Synthetic peptide within Human HGS aa 700 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O14964 -
ポジティブ・コントロール
- WB: HeLa, Renca, CT26.WT and 4T1 whole cell lysate. IP: HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab72053 was affinity purified using an epitope specific to HGS immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72053の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 86 kDa).
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IP |
Use at 2-10 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 86 kDa). |
IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation. -
組織特異性
Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes. -
配列類似性
Contains 1 FYVE-type zinc finger.
Contains 1 UIM (ubiquitin-interacting motif) repeat.
Contains 1 VHS domain. -
ドメイン
Has a double-sided UIM that can bind 2 ubiquitin molecules, one on each side of the helix.
The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions. -
翻訳後修飾
Phosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected (By similarity). Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF.
Ubiquitinated by ITCH. -
細胞内局在
Cytoplasm. Early endosome membrane. Endosome > multivesicular body membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 511582 Cow
- Entrez Gene: 9146 Human
- Omim: 604375 Human
- SwissProt: Q0V8S0 Cow
- SwissProt: O14964 Human
- Unigene: 514590 Human
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別名
- Hepatocyte growth factor regulated tyrosine kinase substrate antibody
- Hepatocyte growth factor-regulated tyrosine kinase substrate antibody
- HGF-regulated tyrosine kinase substrate antibody
see all
画像
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All lanes : Anti-HGS antibody (ab72053) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 86 kDa
Exposure time: 10 secondsLysates prepared using NETN lysis buffer.
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Detection of human HGS by western blot of immunoprecipitates.
Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
Antibodies: ab72053 used for IP at 6 µg per reaction, lanes 1 and 2, lane 3 is control IgG. For blotting immunoprecipitated HGS, ab72053 was used at 0.4 µg/ml. -
All lanes : Anti-HGS antibody (ab72053) at 0.1 µg/ml
Lane 1 : Renca (Mouse renal adenocarcinoma cell line) whole cell lysate
Lane 2 : CT26.WT (Mouse renal colorectal carcinoma cell line) whole cell lysate
Lane 3 : 4T1 (Mouse mammary gland cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 86 kDa
Exposure time: 3 minutesLysates prepared using NETN lysis buffer.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab72053 は 8 報の論文で使用されています。
- Suárez H et al. CD9 inhibition reveals a functional connection of extracellular vesicle secretion with mitophagy in melanoma cells. J Extracell Vesicles 10:e12082 (2021). PubMed: 34012515
- Rojas JM et al. Inhibition of the IFN Response by Bluetongue Virus: The Story So Far. Front Microbiol 12:692069 (2021). PubMed: 34168637
- Avia M et al. Virus-induced autophagic degradation of STAT2 as a mechanism for interferon signaling blockade. EMBO Rep 20:e48766 (2019). PubMed: 31603272
- Torralba D et al. Priming of dendritic cells by DNA-containing extracellular vesicles from activated T cells through antigen-driven contacts. Nat Commun 9:2658 (2018). PubMed: 29985392
- Frattini V et al. A metabolic function of FGFR3-TACC3 gene fusions in cancer. Nature 553:222-227 (2018). PubMed: 29323298
- Gómez-Escudero J et al. E-cadherin cleavage by MT2-MMP regulates apical junctional signaling and epithelial homeostasis in the intestine. J Cell Sci 130:4013-4027 (2017). PubMed: 29061881
- Villarroya-Beltri C et al. ISGylation controls exosome secretion by promoting lysosomal degradation of MVB proteins. Nat Commun 7:13588 (2016). ICC/IF . PubMed: 27882925
- Wang Y et al. Molecular characterization of proprotein convertase subtilisin/kexin type 9-mediated degradation of the LDLR. J Lipid Res 53:1932-43 (2012). PubMed: 22764087