Anti-HGFA Inhibitor 2 抗体 [EPR6283(2)] - BSA and Azide free (ab248225)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6283(2)] to HGFA Inhibitor 2 - BSA and Azide free
- Suitable for: ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-HGFA Inhibitor 2 antibody [EPR6283(2)] - BSA and Azide free
HGFA Inhibitor 2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6283(2)] to HGFA Inhibitor 2 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details
適用なし: IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
ab248225 is the carrier-free version of ab128926.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
解離定数(KD 値)
KD = 7.70 x 10 -11 M Learn more about KD -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6283(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248225の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 28 kDa).
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特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 28 kDa). |
ターゲット情報
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機能
Inhibitor of HGF activator. Also inhibits plasmin, plasma and tissue kallikrein, and factor XIa. -
組織特異性
Expressed in placenta, kidney, pancreas, prostate, testis, thymus, and trachea. -
関連疾患
Defects in SPINT2 are the cause of diarrhea type 3 (DIAR3) [MIM:270420]; also known as congenital sodium diarrhea (CSD). DIAR3 is a rare, inherited diarrhea of infancy. A diagnosis of DIAR3 is made on the findings of a life-threatening secretory diarrhea, severe metabolic acidosis, and hyponatremia secondary to extraordinarily high fecal losses of sodium, with low or normal excretion of urinary sodium, in the absence of infectious, autoimmune, and endocrine causes. -
配列類似性
Contains 2 BPTI/Kunitz inhibitor domains. -
ドメイン
This inhibitor contains two inhibitory domains. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 10653 Human
- Entrez Gene: 20733 Mouse
- Entrez Gene: 292770 Rat
- Omim: 605124 Human
- SwissProt: O43291 Human
- SwissProt: Q9WU03 Mouse
- Unigene: 31439 Human
- Unigene: 295230 Mouse
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別名
- AL024025 antibody
- Bikunin, placental antibody
- C76321 antibody
see all
画像
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All lanes : Anti-HGFA Inhibitor 2 antibody [EPR6283(2)] (ab128926) at 1/1000 dilution
Lane 1 : Fetal kidney tissue lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : Human small intestine lysate
Lane 4 : Caco-2 cell lysate
Lane 5 : PC-3 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 28 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?This data was developed using ab128926, the same antibody clone in a different buffer formulation.
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This data was developed using ab128926, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab248225 は論文での使用が確認できていません。