Anti-HGFA Inhibitor 2 抗体 [EPR6283(2)] (ab128926)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6283(2)] to HGFA Inhibitor 2
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-HGFA Inhibitor 2 antibody [EPR6283(2)]
HGFA Inhibitor 2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6283(2)] to HGFA Inhibitor 2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details
適用なし: IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human HGFA Inhibitor 2 aa 100-200 (extracellular). The exact sequence is proprietary.
-
ポジティブ・コントロール
- WB: Human Caco-2, MCF7, PC-3, BxPC-3, MDA-MB-435S whole cell lysates.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 7.70 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6283(2) -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab128926の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 28 kDa).
|
|
ICC/IF |
1/250 - 1/500.
|
特記事項 |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 28 kDa). |
ICC/IF
1/250 - 1/500. |
ターゲット情報
-
機能
Inhibitor of HGF activator. Also inhibits plasmin, plasma and tissue kallikrein, and factor XIa. -
組織特異性
Expressed in placenta, kidney, pancreas, prostate, testis, thymus, and trachea. -
関連疾患
Defects in SPINT2 are the cause of diarrhea type 3 (DIAR3) [MIM:270420]; also known as congenital sodium diarrhea (CSD). DIAR3 is a rare, inherited diarrhea of infancy. A diagnosis of DIAR3 is made on the findings of a life-threatening secretory diarrhea, severe metabolic acidosis, and hyponatremia secondary to extraordinarily high fecal losses of sodium, with low or normal excretion of urinary sodium, in the absence of infectious, autoimmune, and endocrine causes. -
配列類似性
Contains 2 BPTI/Kunitz inhibitor domains. -
ドメイン
This inhibitor contains two inhibitory domains. -
細胞内局在
Membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 10653 Human
- Entrez Gene: 20733 Mouse
- Entrez Gene: 292770 Rat
- Omim: 605124 Human
- SwissProt: O43291 Human
- SwissProt: Q9WU03 Mouse
- Unigene: 31439 Human
- Unigene: 295230 Mouse
-
別名
- AL024025 antibody
- Bikunin, placental antibody
- C76321 antibody
see all
画像
-
All lanes : Anti-HGFA Inhibitor 2 antibody [EPR6283(2)] (ab128926) at 1/1000 dilution
Lane 1 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4 : BxPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 5 : MDA-MB-435S (Human mammary gland ductal carcinoma melanocyte) whole cell lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST.
-
ab128926, at 1/250 dilution, staining HGFA Inhibitor 2 in MCF-7 cells by Immunofluorescence.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (4)
ab128926 は 4 報の論文で使用されています。
- Steiro I et al. The serine protease matriptase inhibits migration and proliferation in multiple myeloma cells. Oncotarget 13:1175-1186 (2022). PubMed: 36268559
- Ma Z et al. STYK1 promotes tumor growth and metastasis by reducing SPINT2/HAI-2 expression in non-small cell lung cancer. Cell Death Dis 10:435 (2019). PubMed: 31164631
- Roversi FM et al. Up-regulation of SPINT2/HAI-2 by Azacytidine in bone marrow mesenchymal stromal cells affects leukemic stem cell survival and adhesion. J Cell Mol Med N/A:N/A (2018). PubMed: 30484958
- Roversi FM et al. Serine protease inhibitor kunitz-type 2 is downregulated in myelodysplastic syndromes and modulates cell-cell adhesion. Stem Cells Dev 23:1109-20 (2014). PubMed: 24410667