製品の概要

  • 製品名
    Hexokinase Activity Assay Kit (Colorimetric)
    Hexokinase キット 製品一覧
  • サンプルの種類
    Serum, Tissue, Adherent cells, Suspension cells, Tissue Extracts
  • アッセイタイプ
    Quantitative
  • 検出感度
    < 0.1 mU/well
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mammals
  • 製品の概要

    Hexokinase Activity Assay Kit (ab136957) is a product where glucose is converted to glucose-6-phosphate by hexokinase; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm.


    This kit has a simple, sensitive and rapid procedure which can detect hexokinase activity even less than 0.1 mU/well.

  • 特記事項

    Hexokinases (HK) are found in many organisms including bacteria, plants and mammals and play an important role in glucose metabolism. Hexokinases phosphorylate glucose and generate glucose-6-phosphate for glycolysis. Hexokinases have four isoforms (HK-I, II, III and IV). HK-I, HK-II and HK-III have low Km, while HK-IV has 100 fold high Km. Hexokinase deficiency leads to severe human diseases such as X-linked muscular dystrophy and a rare autosomal recessive hemolytic anemia. On the other hand, increased hexokinase activity is detected in various human tumors and is associated with metastasis. Early detection of abnormal hexokinase activity is crucial for diagnosis, prediction and treatment of the disease.

製品の特性

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  • Hexokinase Activity measured in cell lysates showing quantity (nmol) per 1 mln cells after 35 min of incubation.

    Samples with the concentration of 5e6 cells/mL were used. Samples were diluted 1-27 fold.

  • Hexokinase Activity measured in tissue lysates showing quantity (nmol) per mg of extracted protein after 35 min of incubation.

    Protein concentration for samples varied from 13 mg/mL to 43 mg/mL. Samples were diluted 1-9 fold.

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted
  • NADH standard curve (a). Hexokinase activity in Positive Control and Jurkat cell lysate (40 µg) (b).

プロトコール

参考文献

This product has been referenced in:
  • Bramer SA  et al. Hexokinase 2 drives glycogen accumulation in equine endometrium at day 12 of diestrus and pregnancy. Reprod Biol Endocrinol 15:4 (2017). Functional Studies ; Horse . Read more (PubMed: 28056994) »
  • Kim DJ  et al. Increased glucose metabolism and alpha-glucosidase inhibition in Cordyceps militaris water extract-treated HepG2 cells. Nutr Res Pract 11:180-189 (2017). Read more (PubMed: 28584574) »

See all 3 Publications for this product

レビューと Q&A



When preparing these samples please make sure to keep these on ice throughout the preparation procedure. Fresh samples will give you the best results with this kit, however, frozen samples can also be used. If freezing your samples, make sure...

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We have not specifically tested the kit with plasma sample however, it should work with mammalian samples including plasma. It should not affect the sensitivity or accuracy of the test.

This kit is based on function of the Hexokinase enzyme (conversion of glucose to glucose6phosphate is measured) so this kit does not distinguish between the isoforms.

HKI, HKII and HKIII have low Km, while HKIV has 100 fold high Km. Hence it...

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I can confirm the the Hexokinase substrate kit component included in the kit is glucose. This component was recently re-labeled. The error in the naming will be fixed shortly and a new protocol will be available for download from the website shortly. U...

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Yes, this buffer contains NP-40.

The assay buffer contains NP-40.

I apologize for any misunderstanding regarding the unit label for this kit. I am glad you read through the documentation. You are correct in your assessment of how many actual samples can be tested using this kit, after taking into account controls and...

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Unfortunately, the concentration of these components is proprietary information.

Thank you for contacting us.

I have spoken with the lab in regards to these issues. While the positive control information is proprietary, I can confirm that this is a protein control.

Regarding separate purchase of the kit's de...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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