製品名Hexokinase Activity Assay Kit (Colorimetric)
Hexokinase キット 製品一覧
サンプルの種類Serum, Tissue, Adherent cells, Suspension cells, Tissue Extracts
アッセイタイプEnzyme activity (quantitative)
検出感度< 0.1 mU/well
種交差性交差種: Mammals, Other species
Hexokinase Activity Assay Kit (ab136957) uses a simple, sensitive and rapid procedure which can detect hexokinase activity to below 0.1 mU/well.
In the hexokinase assay protocol, glucose is converted to glucose-6-phosphate by hexokinase. The glucose-6-phosphate is then oxidized by glucose-6-phosphate dehydrogenase to form NADH. The NADH reduces a colorless probe to a colored product with strong absorbance at 450 nm.
Hexokinase activity assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 20-60 min
- analyze repeatedly with a microplate reader during the incubation
Hexokinases (HK) are found in many organisms including bacteria, plants and mammals and play an important role in glucose metabolism. Hexokinases phosphorylate glucose and generate glucose-6-phosphate for glycolysis. Hexokinases have four isoforms (HK-I, II, III and IV). HK-I, HK-II and HK-III have low Km, while HK-IV has 100 fold high Km.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
保存方法Store at -20°C. Please refer to protocols.
内容 ラベル 100 tests Assay Buffer WM 1 x 25ml Developer (Lyophilized) Red 1 vial Hexokinase Coenzyme Purple 1 vial Hexokinase Enzyme Mix Green 1 vial Hexokinase Substrate White 1 x 1ml NADH Standard ( lyophilized) Yellow 1 vial Positive Control Orange 1 vial
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Hexokinase Activity measured in cell lysates showing quantity (nmol) per 1 mln cells after 35 min of incubation.
Samples with the concentration of 5e6 cells/mL were used. Samples were diluted 1-27 fold.
Hexokinase Activity measured in tissue lysates showing quantity (nmol) per mg of extracted protein after 35 min of incubation.
Protein concentration for samples varied from 13 mg/mL to 43 mg/mL. Samples were diluted 1-9 fold.
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
NADH standard curve (a). Hexokinase activity in Positive Control and Jurkat cell lysate (40 µg) (b).
In this kit glucose is converted to glucose-6-phosphate by hexokinase; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm.
ab136957 は 8 報の論文で使用されています。
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- Bramer SA et al. Hexokinase 2 drives glycogen accumulation in equine endometrium at day 12 of diestrus and pregnancy. Reprod Biol Endocrinol 15:4 (2017). Functional Studies ; Horse . PubMed: 28056994
- Kim DJ et al. Increased glucose metabolism and alpha-glucosidase inhibition in Cordyceps militaris water extract-treated HepG2 cells. Nutr Res Pract 11:180-189 (2017). PubMed: 28584574
- Albert V et al. mTORC2 sustains thermogenesis via Akt-induced glucose uptake and glycolysis in brown adipose tissue. EMBO Mol Med 8:232-46 (2016). PubMed: 26772600
- Maiso P et al. Metabolic signature identifies novel targets for drug resistance in multiple myeloma. Cancer Res 75:2071-82 (2015). PubMed: 25769724
- Hamabe A et al. Combined evaluation of hexokinase 2 and phosphorylated pyruvate dehydrogenase-E1a in invasive front lesions of colorectal tumors predicts cancer metabolism and patient prognosis. Cancer Sci 105:1100-8 (2014). PubMed: 25060325