Anti-Hes1 抗体 (ab71559)
Key features and details
- Rabbit polyclonal to Hes1
- Suitable for: IHC-Fr, ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Hes1 antibody
Hes1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Hes1 -
由来種
Rabbit -
特異性
This antibody reacts specifically with mouse 30 kDa Hes1 protein. -
アプリケーション
適用あり: IHC-Fr, ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide corresponding to Human Hes1 (C terminal).
Database link: Q14469 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
Constituents: 1.21% Tris, 0.75% Glycine, 2% Sucrose -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab71559の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-Fr | (3) |
Use at an assay dependent concentration.
|
ICC/IF | (4) |
Use at an assay dependent concentration.
|
IHC-P | (5) |
Use at an assay dependent concentration.
|
WB | (14) |
1/200 - 1/2000. Predicted molecular weight: 30 kDa.
|
特記事項 |
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IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
WB
1/200 - 1/2000. Predicted molecular weight: 30 kDa. |
ターゲット情報
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機能
Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity. -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain. -
ドメイン
Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 3280 Human
- Entrez Gene: 15205 Mouse
- Entrez Gene: 29577 Rat
- Omim: 139605 Human
- SwissProt: Q14469 Human
- SwissProt: P35428 Mouse
- SwissProt: Q04666 Rat
- Unigene: 250666 Human
see all -
別名
- bHLHb39 antibody
- C-HAIRY1 antibody
- c-hairy1A antibody
see all
画像
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Anti-Hes1 antibody (ab71559) at 1/2000 dilution + MCF-7 at 20 µg
Secondary
HRP conjugated goat anti-rabbit at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Exposure time: 15 secondsBlocked with 5% milk at RT
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All lanes : Anti-Hes1 antibody (ab71559)
Lane 1 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 1)
Lane 2 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 1)
Lane 3 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 2)
Lane 4 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 2)
Lane 5 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 3)
Lane 6 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 3)
Lane 7 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 4)
Lane 8 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 4)
Predicted band size: 30 kDa
Actin blot given as control.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (126)
ab71559 は 126 報の論文で使用されています。
- Akdemir ES et al. Lunatic Fringe-GFP Marks Lamina-Specific Astrocytes That Regulate Sensory Processing. J Neurosci 42:567-580 (2022). PubMed: 34872929
- Silva E et al. Immunolocalization of Notch Signaling in Mouse Preimplantation Embryos. Methods Mol Biol 2472:173-185 (2022). PubMed: 35674900
- Ni T et al. Medial prefrontal cortex Notch1 signalling mediates methamphetamine-induced psychosis via Hes1-dependent suppression of GABAB1 receptor expression. Mol Psychiatry 27:4009-4022 (2022). PubMed: 35732696
- Shi Y et al. Interaction between BEND5 and RBPJ suppresses breast cancer growth and metastasis via inhibiting Notch signaling. Int J Biol Sci 18:4233-4244 (2022). PubMed: 35844785
- Luan Y et al. REST-repressed lncRNA NPPA-AS1 regulates cervical cancer progression by modulating miR-302e/DKK1/Wnt/β-catenin signaling pathway. J Cell Biochem 122:16-28 (2021). PubMed: 32965043