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Anti-Hepatitis C Virus NS5a 抗体 [H26] (ab13833)

Submit a review Q&A (2)References (8)
Western blot - Anti-Hepatitis C Virus NS5a antibody [H26] (ab13833)

    Key features and details

    • Mouse monoclonal [H26] to Hepatitis C Virus NS5a
    • Suitable for: ELISA, WB, ICC/IF
    • Reacts with: Hepatitis C virus
    • Isotype: IgG1

    製品の概要

    • 製品名

      Anti-Hepatitis C Virus NS5a antibody [H26]

    • 製品の詳細

      Mouse monoclonal [H26] to Hepatitis C Virus NS5a

    • 由来種

      Mouse

    • 特異性

      Hepatitis C Virus antigen NS5a. This antibody works with the HCV genotypes 1a, 1b, 3 and 4, but not with genotype 2a.

    • アプリケーション

      適用あり: ELISA, WB, ICC/IFmore details

    • 種交差性

      交差種: Hepatitis C virus

    • 免疫原

      Recombinant full length protein (E. coli).

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    • バッファー

      pH: 7.40
      Preservative: 0.1% Sodium azide
      Constituent: PBS
    • Concentration information loading...

    • 精製度

      Protein G purified

    • ポリ/モノ

      モノクローナル

    • クローン名

      H26

    • アイソタイプ

      IgG1

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab13833の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    ELISA
    Use at an assay dependent dilution.
    WB
    1/1000.
    ICC/IF
    1/50.
    特記事項
    ELISA
    Use at an assay dependent dilution.
    WB
    1/1000.
    ICC/IF
    1/50.

    ターゲット情報

    • 関連性

      HCV is classified into the genus Hepacivirus of the family Flaviviridae. Like all the members of the family, HCV is an enveloped, single-stranded, positive-sense RNA virus. Its genome (about 9600 nt) is flanked at both termini by conserved, highly structured non-translated regions (NTRs) and encodes a polyprotein precursor (about 3000 aa), which is proteolytically processed by host and viral proteases to produce the structural (core, E1, E2 and p7) and non-structural (NS2, NS3, NS4A, NS4B, NS5A and NS5B) proteins of the virus. Recently, an additional protein has been identified, whose function remains unknown. NS5A is a ~56 kDa pleiotropic protein with key roles in both viral RNA replication and modulation of the physiology of the host cell. It's exact role is not currently known (2008).

    • 参照データベース

      • 別名

        • HCV antibody
        • HCV Non-structural protein 5A antibody
        • Non-structural protein 5A antibody
        • NS5A antibody
        • p56 antibody
        see all

      画像

      • Western blot - Anti-Hepatitis C Virus NS5a antibody [H26] (ab13833)
        Western blot - Anti-Hepatitis C Virus NS5a antibody [H26] (ab13833)Image from Scheel TK et al., PLoS Pathog. 2012 May;8(5):e1002696. Epub 2012 May 24.; Fig 6.; doi:10.1371/journal.ppat.1002696; May 24, 2012, PLoS Pathog 8(5): e1002696.
        Representative western blot detecting Hepatitis C Virus NS5a with ab13833.

        Amounts of Hepatitis C Virus NS5a were compared to Core after spread of infection to >80% of Huh7.5 cells transfected with H77C(1a) and P346A/P351A/P354A, delta414-428 or “delta414-428+20aa” mutants.

      参考文献 (8)

      ab13833 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab13833 は 8 報の論文で使用されています。

      • Shiode Y  et al. Hepatitis C virus enhances Rubicon expression, leading to autophagy inhibition and intracellular innate immune activation. Sci Rep 10:15290 (2020). PubMed: 32943718
      • Li J  et al. Reticulon-3 modulates the incorporation of replication competent hepatitis C virus molecules for release inside infectious exosomes. PLoS One 15:e0239153 (2020). PubMed: 32941510
      • Klinker S  et al. Phosphorylated tyrosine 93 of hepatitis C virus nonstructural protein 5A is essential for interaction with host c-Src and efficient viral replication. J Biol Chem 294:7388-7402 (2019). PubMed: 30862675
      • Kuwashiro T  et al. Regulation of interferon signaling and HCV-RNA replication by extracellular matrix. Int J Mol Med 42:957-965 (2018). PubMed: 29786754
      • Madhvi A  et al. A screen for novel hepatitis C virus RdRp inhibitor identifies a broad-spectrum antiviral compound. Sci Rep 7:5816 (2017). PubMed: 28725041
      • Abdallah C  et al. Hepatitis C virus core protein targets 4E-BP1 expression and phosphorylation and potentiates Myc-induced liver carcinogenesis in transgenic mice. Oncotarget 8:56228-56242 (2017). PubMed: 28915586
      • Meistermann H  et al. A novel immuno-competitive capture mass spectrometry strategy for protein-protein interaction profiling reveals that LATS kinases regulate HCV replication through NS5A phosphorylation. Mol Cell Proteomics 13:3040-8 (2014). PubMed: 25044019
      • Scheel TK  et al. Analysis of functional differences between hepatitis C virus NS5A of genotypes 1-7 in infectious cell culture systems. PLoS Pathog 8:e1002696 (2012). WB ; Hepatitis C virus . PubMed: 22654662

      レビューと Q&A

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      1-2 of 2 Abreviews or Q&A

      Question

      Dear Sir/Madam,

      I have a question regarding storage of two antibodies bought from you,a b13830 and ab13833. Due to a misunderstanding, the antibodies have been stored at +4 C, even though the product sheet recommends storing at –20 C. The antibodies have been stored at +4 C approximately two weeks. Do you know if this will affect the products negatively?

      Yours sincerely,

      Read More
      Answer

      Thank you for your message.

      Reviewing the recommended storage details for these antibodies, I can suggest they should not be significantly affected by short term storage for a week or two at +4oC. However, I would recommend to aliquot and freeze them as soon as possible.

      We would recommend that products are stored as recommended on the datasheet as soon as they are delivered. This will ensure the product remains stable and that we can continue to provide our guarantee.

      I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

      Read More

      Question

      Could you tell me whether the epitope of the following antibody is known: Hepatitis C Virus NS5a antibody [H26] (ab13833) Thank you

      Read More
      Answer

      Thank you for your enquiry. I can confirm that the exact epitope is not know, but it has been mapped between amino acid positions 2200 and 2250. I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

      Read More

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