The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. A major band is obtained at ~50 kDa with minor bands at ~35, ~40, and ~67 kDa. This reflects bands found in DTT reduced Blots of the immunizing antigen and in immunoblots using a known antibody positive serum.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.
NS5 (non structural protein 5) may play a role in the viral RNA replication of the Hepatitis C Virus.