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Anti-Hepatitis C Virus NS3 抗体 [H23] (ab13830)

Submit a review Q&A (4)References (33)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis C Virus NS3 antibody [H23] (ab13830)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis C Virus NS3 antibody [H23] (ab13830)
  • Western blot - Anti-Hepatitis C Virus NS3 antibody [H23] (ab13830)

Key features and details

  • Mouse monoclonal [H23] to Hepatitis C Virus NS3
  • Suitable for: ELISA, WB, IHC-P
  • Reacts with: Hepatitis C virus
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Hepatitis C Virus NS3 antibody [H23]
    Hepatitis C Virus NS3 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [H23] to Hepatitis C Virus NS3

  • 由来種

    Mouse

  • 特異性

    Hepatitis C Virus antigen NS3 helicase domain. Reacts very well with JFH-1 strain (genotype 2a)

  • アプリケーション

    適用あり: ELISA, WB, IHC-Pmore details

  • 種交差性

    交差種: Hepatitis C virus

  • 免疫原

    Recombinant full length protein corresponding to Hepatitis C Virus NS3.

  • エピトープ

    Helicase domain

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • バッファー

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Protein G purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    H23

  • アイソタイプ

    IgG1

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab13830の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
Use at an assay dependent concentration.
WB
1/1000.
IHC-P
1/50.

Tested using paraffin-embedded liver sections from HCV patients.
特記事項
ELISA
Use at an assay dependent concentration.
WB
1/1000.
IHC-P
1/50.

Tested using paraffin-embedded liver sections from HCV patients.

ターゲット情報

  • 関連性

    HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.

  • 細胞内局在

    Endoplasmic reticulum membrane

  • 参照データベース

    • 別名

      • Hepatitis C Virus NS3 antibody
      • NS3 antibody
      • NS3P antibody
      • p70 antibody
      • Serine protease/NTPase/helicase antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis C Virus NS3 antibody [H23] (ab13830)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis C Virus NS3 antibody [H23] (ab13830)
      ab13830 used in IHC at 1/50 dilution. Cell line used is Huh7 human hepatoma.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis C Virus NS3 antibody [H23] (ab13830)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis C Virus NS3 antibody [H23] (ab13830)
      The negative control for above picture with Huh7 cells.
    • Western blot - Anti-Hepatitis C Virus NS3 antibody [H23] (ab13830)
      Western blot - Anti-Hepatitis C Virus NS3 antibody [H23] (ab13830)Image from Kota S et al., PLoS One. 2012;7(2):e32207. Epub 2012 Feb 28.; Fig 3.; doi:10.1371/journal.pone.0032207; February 28, 2012, PLoS ONE 7(2): e32207.
      Representative Western Blot detecting Hepatitis C Virus NS3 with ab13830.

      SL209-biotin was immobilized on streptavidin agarose beads and incubated with lysates of Huh-7.5 cells infected with HCV J6/JFH-1. Retained proteins were examined by western blot.

    参考文献 (33)

    ab13830 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab13830 は 33 報の論文で使用されています。

    • Zou LL  et al. TGF-ß isoforms inhibit hepatitis C virus propagation in transforming growth factor beta/SMAD protein signalling pathway dependent and independent manners. J Cell Mol Med 25:3498-3510 (2021). PubMed: 33682288
    • Li J  et al. Reticulon-3 modulates the incorporation of replication competent hepatitis C virus molecules for release inside infectious exosomes. PLoS One 15:e0239153 (2020). PubMed: 32941510
    • Ma YY  et al. IL28A protein homotetramer structure is required for autolysosomal degradation of HCV-NS5A in vitro. Cell Death Dis 11:200 (2020). PubMed: 32205851
    • Xu L  et al. Tupaia MAVS Is a Dual Target during Hepatitis C Virus Infection for Innate Immune Evasion and Viral Replication via NF-?B. J Immunol 205:2091-2099 (2020). PubMed: 32907995
    • Zhang X  et al. Establishment and transcriptomic features of an immortalized hepatic cell line of the Chinese tree shrew. Appl Microbiol Biotechnol 104:8813-8823 (2020). PubMed: 32880691
    View all Publications for this product

    レビューと Q&A

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    レビューを送る 質問を送る

    1-4 of 4 Abreviews or Q&A

    Question

    Dear Sir/Madam,

    I have a question regarding storage of two antibodies bought from you,a b13830 and ab13833. Due to a misunderstanding, the antibodies have been stored at +4 C, even though the product sheet recommends storing at –20 C. The antibodies have been stored at +4 C approximately two weeks. Do you know if this will affect the products negatively?

    Yours sincerely,

    Read More
    Answer

    Thank you for your message.

    Reviewing the recommended storage details for these antibodies, I can suggest they should not be significantly affected by short term storage for a week or two at +4oC. However, I would recommend to aliquot and freeze them as soon as possible.

    We would recommend that products are stored as recommended on the datasheet as soon as they are delivered. This will ensure the product remains stable and that we can continue to provide our guarantee.

    I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

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    Question

    Hi,   I am interested in the hepatitis C anti-NS3 antibody for WB. I would like to check the expression condition of the NS3 protein using the antibody, especially its helicase domain.  Would you suggest any antibody fit for this purpose? I have rabbit/mouse HRP secondary antibody. Thanks.   Yi

    Read More
    Answer

    Thank you for your enquiry. I am sorry it has taken some time to request the immunogen seuqences from our sources and review these for you, thank you for your patience. I can confirm the following mouse Hepatitis C Virus NS3 antibodies have been tested in wesern blot and should detect the helicase domain. I can recommend to review the online datasheets for further information: ab13830 Hepatitis C Virus NS3 antibody [H23] Mouse monoclonal Applications: ELISA, IHC-P, WB The immunogen for this mab was of the genotype 1b, the exact sequence is unknown. The epitope has been mapped to the helicase domain (between aa positions 1350 and1500). Click here (or use the following: https://www.abcam.com/Hepatitis-C-Virus-NS3-antibody-H23-ab13830.html). ab65407 Hepatitis C Virus NS3 antibody [8 G-2] Mouse monoclonal Applications: ELISA, ICC/IF, IHC-P, WB The epitope has been mapped between amino acid positions 1340 and 1480 The antibody includes and recognises the helicase domain. Click here (or use the following: https://www.abcam.com/Hepatitis-C-Virus-NS3-antibody-8-G-2-ab65407.html). I am still waiting for confirmation of the immunogen from several more antibodies. I will contact you again if I have any further information that any will detect the helicase domain. I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

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    Question

    here is the scan of the blot. there is a good band but unfortunately it shows up in the negative control which is the 20.5 and does not show up in the positive control using NS3 protein only. let me know what you think.

    Read More
    Answer

    Thank you for sending me the image of your blot. So, you have no bands (just some background) in the NS3 recombinant protein lane, and the same pattern of bands in the other lanes, including the negative control. Just to make sure, do you know that your secondary antibody is working properly? Also, have you tried in the past another Hepatitis C Virus antigen antibody with these samples?

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    Question

    BATCH NUMBER 64192 ORDER NUMBER 66184 DESCRIPTION OF THE PROBLEM No signal or weak signal SAMPLE Huh 20.5 (negative control) Huh BB7, GS4.1 (positive for HCV NS3) NS3 recombinant protein (positive control) Core recombinant protein (negative control) PRIMARY ANTIBODY abcam 13830 mouse monoclonal at 1:1000 and 1:500 for two hours, wash five to ten minutes. SECONDARY ANTIBODY secondary anti-mouse 1:10,000 for 2 hours and wash twice for 30 mins DETECTION METHOD ECL for 5 minutes ANTIBODY STORAGE CONDITIONS 4*C SAMPLE PREPARATION lysis buffer 300mM NaCl, 50mM Tris, 5mM EDTA, 5% Igepal, 1mMPMSF, 5ug/ml leupeptin, 30ug/ml aproptonin. lyse on ice for 10 minutes, spin and collect supernatants. AMOUNT OF PROTEIN LOADED add 10-20 ug protein with 2X SDS reducing buffer ELECTROPHORESIS/GEL CONDITIONS load and run on 10% SDSPAGE run for one and a half hours and transfer TRANSFER AND BLOCKING CONDITIONS transfer 2 hours and block using milk, tween 20 and pbs HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? amount of primary antibodyand length of time. Hi I actually purchased this antibody the hepatitis C virus antigen and tested the Western Blot specificity using a control containing HCV NS3 positive and a negative control and did not receive a specific band in the correct size. I performed the WB at both the suggested dilution of antibody 1:1000 and at 1:500. neither blot worked. There was proper transfer of protein but too many non-specific bands and non at the specific size. Is there another antibody that I can get as a replacement or a refund?? please let me know asap, I can send you the blot as an attachment if you want to see.

    Read More
    Answer

    Thank you for your message and I'm sorry to hear that you are experiencing difficulty with ab13830. It would be helpful if you could send me an image of the blot. You mention no signal but then mention non-specific bands. How strong are these bands? What are you seeing in your negative control lanes? At this point I would like to make a few suggestions that will hopefully help you out. Try incubating with the primary at a dilution of 1:1000 overnight at 4C. Also, you mention that you are seeing non-specific bands; make sure to run a secondary only control (no primary antibody) to ensure that these bands are not due to your secondary antibody. Thanks, and I look forward to hearing from you.

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