アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • カスタム製品 & パートナーシップ
    カスタム製品 & パートナーシップ

    診断や治療プログラムを加速するための、カスタム製品および商業化に向けたパートナーシップ

    カスタム製品

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  1. Link

    hepatitis-c-virus-core-1b-antibody-c7-50-ab2740.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Microbiology Organism Virus RNA Virus ssRNA positive strand virus HCV
Share by email

Anti-Hepatitis C Virus Core 1b 抗体 [C7-50] (ab2740)

  • Datasheet
  • SDS
Reviews (3)Q&A (2)References (82)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Shipping info

Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Key features and details

  • Mouse monoclonal [C7-50] to Hepatitis C Virus Core 1b
  • Suitable for: IP, Flow Cyt, ELISA, WB
  • Reacts with: Hepatitis C virus
  • Isotype: IgG1

こちらの製品もご検討ください

アッセイ
Product image
Propidium Iodide Flow Cytometry Kit (ab139418)
Biochemical
Product image
TEMED, free radical stabilizer (ab146235)
二次抗体
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

関連製品

製品の概要

  • 製品名

    Anti-Hepatitis C Virus Core 1b antibody [C7-50]
  • 製品の詳細

    Mouse monoclonal [C7-50] to Hepatitis C Virus Core 1b
  • 由来種

    Mouse
  • 特異性

    Detects hepatitis C virus (HCV) core protein from transfected human and primate cell lines.
  • アプリケーション

    適用あり: IP, Flow Cyt, ELISA, WBmore details
  • 種交差性

    交差種: Hepatitis C virus
  • 免疫原

    Recombinant full length protein (GST-tag) corresponding to Hepatitis C Virus Core 1b.

  • エピトープ

    This antibody recognizes an epitope between amino acid residues 21-40 of HCV core protein. This sequence is conserved among different HCV strains.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    C7-50
  • アイソタイプ

    IgG1
  • 研究分野

    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • HCV
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • Hepatitis A/C/E/G

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab2740の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
Use at an assay dependent concentration.
Flow Cyt
1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ELISA
Use at an assay dependent concentration.
WB (1)
Use a concentration of 1 µg/ml. This antibody detects a single ~21 kDa protein representing HCV core protein in various transfected cell lines.
特記事項
IP
Use at an assay dependent concentration.
Flow Cyt
1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ELISA
Use at an assay dependent concentration.
WB
Use a concentration of 1 µg/ml. This antibody detects a single ~21 kDa protein representing HCV core protein in various transfected cell lines.

ターゲット情報

  • 関連性

    HCV (Hepatitis C Virus) viral core protein forms the internal viral coat that encapsidates the genomic RNA and is enveloped in a host cell-derived lipid membrane. The hepatitis C virus (HCV) core protein represents the first 177 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum. The N terminus of the core protein is involved in transcriptional repression. There are over 20 different subtypes of Hepatitis C Virus; HCV type 1b is mostly found in Europe and Asia. The prevalence of HCV type 1b infection has recently decreased, although it still accounts for most HCV-related cirrhosis and hepatocellular carcinoma. High HCV viremia levels and HCV genotype type 1b are independent predictors for poor response to interferon-alpha therapy. HCV core protein is among the most conserved proteins in HCV and is known to induce sensitization of cytotoxic T lymphocytes (CTL). Therefore, it is a prime candidate for a component of a potential HCV vaccine.
  • 細胞内局在

    Secreted; Host endoplasmic reticulum membrane; Single-pass membrane protein; Host nucleus; Host cytoplasm.
  • 参照データベース

    • Entrez Gene: 951475 Hepatitis C virus
    • SwissProt: P26662 Hepatitis C virus
    • SwissProt: P26663 Hepatitis C virus
    • SwissProt: Q9WMX2 Hepatitis C virus
    • 別名

      • Capsid protein C antibody
      • Core protein p19 antibody
      • Core protein p21 antibody
      • Hepatitis C virus core protein antibody

    プロトコール

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (82)

    ab2740 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2740 は 82 報の論文で使用されています。

    • Zou LL  et al. TGF-ß isoforms inhibit hepatitis C virus propagation in transforming growth factor beta/SMAD protein signalling pathway dependent and independent manners. J Cell Mol Med 25:3498-3510 (2021). PubMed: 33682288
    • Xiao D  et al. Mechanisms of microRNA-142 in mitochondrial autophagy and hippocampal damage in a rat model of epilepsy. Int J Mol Med 47:N/A (2021). PubMed: 33846769
    • Sun HY  et al. Sterol O-acyltransferase 2 chaperoned by apolipoprotein J facilitates hepatic lipid accumulation following viral and nutrient stresses. Commun Biol 4:564 (2021). PubMed: 33980978
    • Ma YY  et al. IL28A protein homotetramer structure is required for autolysosomal degradation of HCV-NS5A in vitro. Cell Death Dis 11:200 (2020). PubMed: 32205851
    • Zhang MQ  et al. A new transcription factor ATG10S activates IFNL2 transcription by binding at an IRF1 site in HepG2 cells. Autophagy 16:2167-2179 (2020). PubMed: 31996071
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-5 of 5 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Hepatitis C Virus Core 1b antibody [C7-50]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (human hepatocarcinoma)
    Antigen retrieval step
    None
    Permeabilization
    Yes - H2O2
    Specification
    human hepatocarcinoma
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 0.05% · Temperature: 24°C
    Fixative
    Methanol
    Read More

    Hwanhee Lee

    Verified customer

    投稿 Jul 01 2016

    Western blot abreview for Anti-Hepatitis C Virus Core 1b antibody [C7-50]

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (12%)
    Sample
    Human Cell lysate - whole cell (human liver carcinoma cell line)
    Specification
    human liver carcinoma cell line
    Treatment
    transfection of Core protein expressed vector
    Blocking step
    BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    投稿 Oct 29 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Hepatitis C Virus Core Antigen antibody [C7-50]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Huh7-Lunet Cells)
    Specification
    Huh7-Lunet Cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Triton X-100
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 19 2013

    Question

    https://www.abcam.com/Hepatitis-C-Virus-Core-Antigen-antibody-C7-50-ab2740.html

    This is my antibody sir

    Read More

    Abcam community

    Verified customer

    Asked on May 24 2012

    Answer

    Thank you for confirming the details of this product.

    I am pleased to provide thetesting protocol for ELISA using this antibody which I have copied below. Please note, this is a guideline only and may require some individual optimization.

    While we do not recommend an ELISA specific dilution for this antibody,a usefulstandard starting point for recommendations for ELISA antibody concentrations is 1-10ug/ml for coating and 1-5ug/ml for detection.

    I hope the following article may also be helpful to you:

    J Med Virol. 1996 Mar;48(3):234-41.
    Characterization of three novel monoclonal antibodies against hepatitis C virus core protein.

    Author(s): Moradpour D, Wakita T, Tokushige K, Carlson RI, Krawczynski K, Wands JR
    (http://www.ncbi.nlm.nih.gov/pubmed/8801283)

    I am sorry to confirm that the cut off point has not been determiened. Calculation of the results and statistics will depend on what you require. I can recommend to review further details in the instructions for the platereader software regarding data analysis. I am sorry we have no more specific information to provide.

    I hope this will be helpful. If you have any further questions, please do not hesitate to contact me.

    General ELISA Protocol

    1. Bind Antigen to Microplate

    a. Dispense 1-5 mg antigen in PBS into each well.
    b. Cover the plate with parafilm and place in the refrigerator overnight.
    c. Remove and discard antigen solution from plate.

    2. Blocking and Primary Antibody Incubation

    a. Block each well in the entire microplate by adding 200 ml of 2.5 mg/ml BSA in PBS to each well.
    b. Allow plate to stand at room temperature for 2 hours.
    c. Remove and discard blocking solution from plate.
    d. Add the appropriate amount of primary antibody to each well and incubate at room temperature for 1 hour.
    e. Remove and discard incubation solution from plate.
    f. Wash each well using TBST (TBS containing 0.1% Tween20). Do NOT cross-contaminate wells.
    g. Repeat washing step for a total of 5 washes.

    3. Secondary Incubation and Detection

    a. Add appropriate amount of secondary antibody solution wells and incubate at room temperature for 1 hour.
    b. Remove and discard secondary antibody solution from plate.
    c. Wash each well using TBST (TBS containing 0.1% Tween20). Do NOT cross-contaminate wells.
    d. Repeat washing step for a total of 5 washes.
    e. Add the appropriate detection reagent, incubate, and stop the color development according to manufacturer’s recommendations.
    f. Read the plate according to manufacturer’s recommendations.

    Read More

    Abcam Scientific Support

    Answered on May 24 2012

    Question

    will this antibody (ab2740) recognize genotype 1a virus? Thanks in advance for your help!

    Read More

    Abcam community

    Verified customer

    Asked on Jan 05 2004

    Answer

    It is not known. From epitope mapping it has been shown to be directed against aa 21-40 of the HCV core region. I would suggest seeing how conserved this sequence is in 1a.

    Read More

    Abcam Scientific Support

    Answered on Jan 06 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    技術情報や製品情報をメールで受け取る 登録
    製品・研究分野別
    • 癌
    • 循環器
    • 細胞生物学
    • 発生
    • エピジェネティクス&核内情報
    • 免疫学
    • メタボリズム
    • 感染症
    • ニューロサイエンス
    • シグナル伝達
    • ステムセル
    製品・タイプ別
    • 一次抗体
    • 二次抗体
    • バイオケミカルズ
    • アイソタイプ・コントロール
    • マルチカラー・セレクター
    • キット
    • ローディング・コントロール
    • ライゼート
    • ペプチド
    • タンパク質
    • スライド
    • タグ抗体・細胞マーカー
    • その他試薬
    サービス&サポート
    • サポート
    • よくあるお問い合わせ
    • プロトコール&トラブルシュート
    • ご購入に関して
    • お問い合わせフォーム
    • トレーニング
    • ターゲット別に見る
    アブカムについて
    • 企業ウェブサイト
    • 投資家の皆様へ
    • 企業情報
    • 採用情報
    • アブカムについて
    イベント
    • 展示会
    • カンファレンス
    International websites
    • abcam.com
    • abcam.cn

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    © 1998-2022 Abcam plc. All rights reserved.