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Microbiology Interspecies Interaction Host Virus Interaction
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Anti-Hepatitis B Virus X antigen 抗体 [X36C] (ab2741)

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Submit a review Q&A (5)References (7)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-Hepatitis B Virus X antigen antibody [X36C] (ab2741)

    Key features and details

    • Mouse monoclonal [X36C] to Hepatitis B Virus X antigen
    • Suitable for: IHC-P, ELISA, IP, WB
    • Reacts with: Hepatitis B virus
    • Isotype: IgG1

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    関連製品

    製品の概要

    • 製品名

      Anti-Hepatitis B Virus X antigen antibody [X36C]
      Hepatitis B Virus X antigen 一次抗体 製品一覧
    • 製品の詳細

      Mouse monoclonal [X36C] to Hepatitis B Virus X antigen
    • 由来種

      Mouse
    • アプリケーション

      適用あり: IHC-P, ELISA, IP, WBmore details
    • 種交差性

      交差種: Hepatitis B virus
    • 免疫原

      Recombinant full length protein corresponding to Hepatitis B Virus X antigen.

    • 特記事項

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • バッファー

      Preservative: 0.05% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • 精製度

      HPTLC
    • 一次抗体 備考

      Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein-protein interaction.
    • ポリ/モノ

      モノクローナル
    • クローン名

      X36C
    • アイソタイプ

      IgG1
    • 研究分野

      • Microbiology
      • Interspecies Interaction
      • Host Virus Interaction
      • Microbiology
      • Organism
      • Virus
      • DNA Virus
      • double stranded DNA Virus
      • Hepatitis B

    関連製品

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Conjugation kits

      • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

    アプリケーション

    Our Abpromise guarantee covers the use of ab2741 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    IHC-P Use at an assay dependent concentration.
    ELISA Use at an assay dependent concentration.
    IP Use at an assay dependent concentration.
    WB Use a concentration of 1 µg/ml.

    ターゲット情報

    • 関連性

      Hepatitis B virus X protein (HBx) is a 17 kD transcriptional coactivator that plays a significant role in the regulation of genes involved in inflammation and cell survival. It regulates many transcription factors including nuclear factor kappa B (NF-kappaB) and plays a key role in hepatocarcinogenesis. HBx facilitates the binding of cAMP response element binding protein (CREB) to its responsive element. HBx stabilizes the cellular coactivator ASC-2 through direct protein-protein interaction, affecting the regulation of genes actively transcribed in liver cancer cells. HBx transactivates both JNK and MAPK signal transduction pathways in association with the mobilization of cytosolic Ca2+. The communication between HBx and general transcription factor TFIIB is also one of the mechanisms which account for its transcriptional transactivation. HBx decreased the expression of PTEN a known tumor suppressor and a negative regulator of phosphatidylinositol 3'-kinase/AKT and HBx decreased the expression of PTEN in HBx-transfected cells. The etiology of hepatocellular carcinoma (HCC) is involved with hepatitis B virus (HBV) infection and HBx in particular plays a role in the development of HBV-related HCC. The persistence of HBx is important to the pathogenesis of early HCC and HBx expression in the liver during chronic HBV infection may be an important prognostic marker for the development of HCC.
    • 参照データベース

      • SwissProt: O91531 Hepatitis B virus
      • SwissProt: Q4R1S1 Hepatitis B virus
      • SwissProt: Q91C38 Hepatitis B virus
      • 別名

        • HBV X antigen antibody
        • HBx antibody
        • HBxAg antibody

      画像

      • Western blot - Anti-Hepatitis B Virus X antigen antibody [X36C] (ab2741)
        Western blot - Anti-Hepatitis B Virus X antigen antibody [X36C] (ab2741)Image from Yip WK et al., PLoS One. 2011;6(8):e22888. Epub 2011 Aug 4. Fig 2.; doi:10.1371/journal.pone.0022888; August 4, 2011, PLoS ONE 6(8): e22888.
        All lanes : Anti-Hepatitis B Virus X antigen antibody [X36C] (ab2741) at 1/2000 dilution

        Lane 1 : MIHA hepatocytes
        Lane 2 : MIHA hepatocytes infected with lentivirus containing EGFP alone
        Lane 3 : MIHA hepatocytes infected with lentivirus containing full length Hepatitis B Virus X antigen
        Lanes 4-5 : MIHA hepatocytes infected with lentivirus containing truncated Hepatitis B Virus X antigen

      プロトコール

      • Immunoprecipitation protocols
      • Immunohistochemistry protocols
      • Western blot protocols

      Click here to view the general protocols

      データシートおよび資料

      • Datasheet
    • 参考文献 (7)

      ab2741 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab2741 は 7 報の論文で使用されています。

      • You DG  et al. Hepatitis B virus X protein induces size-selective membrane permeabilization through interaction with cardiolipin. Biochim Biophys Acta Biomembr 1861:729-737 (2019). PubMed: 30658058
      • Zha Y  et al. Hepatitis B virus X protein promotes epithelial-mesenchymal transition and metastasis in hepatocellular carcinoma cell line HCCLM3 by targeting HMGA2. Oncol Lett 16:5709-5714 (2018). PubMed: 30356986
      • Lee HR  et al. A direct role for hepatitis B virus X protein in inducing mitochondrial membrane permeabilization. J Viral Hepat 25:412-420 (2018). PubMed: 29193612
      • Wang B  et al. Hepatitis B virus infection is not associated with fatty liver disease: Evidence from a cohort study and functional analysis. Mol Med Rep N/A:N/A (2018). PubMed: 30387826
      • Zhong L  et al. Reactive Oxygen Species-Mediated c-Jun NH2-Terminal Kinase Activation Contributes to Hepatitis B Virus X Protein-Induced Autophagy via Regulation of the Beclin-1/Bcl-2 Interaction. J Virol 91:N/A (2017). PubMed: 28515304
      • Huang P  et al. Hepatitis B Virus X Protein (HBx) Is Responsible for Resistance to Targeted Therapies in Hepatocellular Carcinoma: Ex Vivo Culture Evidence. Clin Cancer Res 21:4420-30 (2015). PubMed: 26059188
      • Yip WK  et al. Carboxyl-terminal truncated HBx regulates a distinct microRNA transcription program in hepatocellular carcinoma development. PLoS One 6:e22888 (2011). WB . PubMed: 21829663

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-5 of 5 Abreviews or Q&A

      Question

      Customer requested positive control for ab2741?

      Read More

      Abcam community

      Verified customer

      Asked on Nov 28 2012

      Answer

      Thank you for contacting us.

      Unfortunately laboratory has confirmed that they will not be able to provide the positive control. I am sorry I am not able to help further with this inquiry.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

      Read More

      Abcam Scientific Support

      Answered on Nov 28 2012

      Question

      Dear Sir or Madam,

      We are a biophysical lab and we are working with recombinantly expressed HBV proteins. To validate some of our recombinant protein we're now looking into co-immunoprecipitation and western blotting. These techniques have never been used in our lab, so I have a few questions. To establish antibody techniques in our lab I am interested in HBx antibodies, but once protocols have been established we'd be interested in antibodies against other HBV proteins as well.

      Could you send me a quote for the antibodies listed below?


      Anti-6X His tag® antibody [3H2201] (ab15149)

      Anti-6X His tag® antibody (ab125262)

      Anti-6X His tag® antibody (Agarose) (ab90663)

      Anti-Hepatitis B Virus X antigen antibody [3F6-G10] (ab235)
      Anti-Hepatitis B Virus X antigen antibody [X36C] (ab2741)
      Anti-Hepatitis B Virus X antigen antibody (ab39716)

      Anti-p53 antibody [PAb 240] (ab26)

      I'm also interested in secondary antibodies for the use in western blots. Could you recommend a matching secondary anti-mouse antibody for WB use?
      WouldRabbit anti-Mouse IgG H&L (HRP) secondary antibody (ab7075) be a good choice?

      Reading the posts in your support section I can see that the epitopes of the HBx antibodies are not known. Has anything changed since then?
      And my last question would be if you supply any conformation specific antibodies against HBV core protein (HBc).

      Best wishes

      Read More

      Abcam community

      Verified customer

      Asked on Sep 03 2012

      Answer

      Sorry for the delay in getting back to you. I now have further information to share with you.

      I have checked into whether anything is know about the epitope of the anti-Hepatitis B Virus X antigen antibodies ab235, ab2741 and ab39716. I can only provide further information for ab235 additionally to the information provided on the datasheet of the antibodies. The epitope recognised by ab235 is thought to recognise an epitope of the following sequence:

      DVLCLRPVGASESR.

      I was incorrect in my last email to say that ab235 had not been tested in Western blotting. It have been tested but we have had mixed reports from customers so we would not recommend buying this antibody with that application in mind.

      I have also looked into the anti-Hepatitis B Virus Core Antigen antibodies we have and if anything is known of the conformation of the epitope is known. Unfortunately, I could not find any antibodies where this information had been established and most are able to recognise both the denatured and native form of the protein.

      I hope this information has been of help. If you have any further questions, please do not hesitate to ask.

      Read More

      Abcam Scientific Support

      Answered on Sep 03 2012

      Question

      Dear Sir or Madam,

      We are a biophysical lab and we are working with recombinantly expressed HBV proteins. To validate some of our recombinant protein we're now looking into co-immunoprecipitation and western blotting. These techniques have never been used in our lab, so I have a few questions. To establish antibody techniques in our lab I am interested in HBx antibodies, but once protocols have been established we'd be interested in antibodies against other HBV proteins as well.

      Could you send me a quote for the antibodies listed below?


      Anti-6X His tag® antibody [3H2201] (ab15149)

      Anti-6X His tag® antibody (ab125262)

      Anti-6X His tag® antibody (Agarose) (ab90663)

      Anti-Hepatitis B Virus X antigen antibody [3F6-G10] (ab235)
      Anti-Hepatitis B Virus X antigen antibody [X36C] (ab2741)
      Anti-Hepatitis B Virus X antigen antibody (ab39716)

      Anti-p53 antibody [PAb 240] (ab26)

      I'm also interested in secondary antibodies for the use in western blots. Could you recommend a matching secondary anti-mouse antibody for WB use?
      WouldRabbit anti-Mouse IgG H&L (HRP) secondary antibody (ab7075) be a good choice?

      Reading the posts in your support section I can see that the epitopes of the HBx antibodies are not known. Has anything changed since then?
      And my last question would be if you supply any conformation specific antibodies against HBV core protein (HBc).

      Best wishes

      Read More

      Abcam community

      Verified customer

      Asked on Aug 28 2012

      Answer

      Thank you for contacting us and your interest in our products.

      The request for a quote for the antibodies mentioned has been passed on to one of my colleagues who will get back to you shortly with this information. In regards to your other questions:

      The Rabbit anti-Mouse IgG H&L (HRP) secondary antibody (ab7075) should work well with ab15149, ab2741, ab235 and ab26. You would need a different secondary antibody for all the other antibodies mentioned as they are raised in rabbit. You may wantto consider the Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080). Please bear in mind that we have as yet not tested the anti-Hepatitis B Virus X antigen antibody [3F6-G10] (ab235) in Western blotting or immunoprecipitation. This does not mean that it would not work, simply that we have not tested it ourselves as yet and would therefore not he able to guarantee its performance in these applications.

      We have some good resources in setting up both immunoprecipitation and Western blotting experiments from the following link:

      https://docs.abcam.com/pdf/misc/abcam-protocols-book-2010.pdf

      Some further troubleshooting tips can also be found here:

      https://www.abcam.com/index.html?pageconfig=resource&rid=11382

      If you would like any help with this please do let us know.

      I will check if any further information is know about the epitopes recognised by the anti-HBx antibodies mentioned and will get back to you with this information as soon as I have it. I will also look into your final question ifwe have any conformation specific antibodies against HBV core protein (HBc).

      As you will be setting up these experiments from scratch, you may be interested in looking at our Western blotting range:

      https://www.abcam.com/index.html?pageconfig=resource&rid=14709&source=pagetrap&viapagetrap=optiblot

      The link above will take you not only to our Optiblot range of gels which provide superior durability and ease of use compared to many standard gels, but also the PVDF and nitrocellulose membranes and Ecl Detection kits we have available.

      If you would like any further information on these products please do let us know.

      Read More

      Abcam Scientific Support

      Answered on Aug 28 2012

      Question

      Our customer some questions with antibodies ab2741, ab235, ab39716. He want to know which subtype virus that these antibodis can detect? Does these antibodis detect nature protein or denatue protein?

      Read More

      Abcam community

      Verified customer

      Asked on Sep 26 2007

      Answer

      Thank you for your message. Unfortuantley, I am unable to obtain precise information about which subtypes of Hepatitis B virus these antibodies detect. We aim to provide as much information as possible to customers, and I am sorry this is not possible on this occasion. With regards to whether the antibodies detect native or denatured protein, you should always use reducing and denaturing conditions unless stated otherwise on the datasheet. Please also check the information I have provided below for futher details. I did manage to obtain the following information on these antibodies, which I hope you will find helpful: Reference for ab2741. This reference uses the antibody in IP and WB procedures. M. Melegari, et al. Cloning and Characterization of a Novel Hepatitis B Virus x Binding Protein That Inhibits Viral Replication. J. Virology, 72(3): 1737-1743, 1998. http://jvi.asm.org/cgi/content/full/72/3/1737 Hepatitis B Virus X antigen antibody ab39716 was tested only with recombinant antigen in WB (reducing and nonreducing) and for immunoprecipitation: with antigen from HeLa cells transfected with HBX tagged with c-myc. Antibody ab235 reacts with both the native and denatured HBX antigens. For further details of this clone please see the following references: Pal J, Somogyi C, Szmolenszky A A, Szekeres G, Sipos J, Hegedus G, Martzinovits I, Molnar J, Nemeth P. Immunohistochemical assessment and prognostic value of hepatitis B virus X protein in chronic hepatitis and primary hepatocellular carcinomas using anti-HBxAg monoclonal antibody. Pathol Oncol Res. 2001;7(3):178-84. PMID: 11692143 Marczinovits I, Somogyi C, Patthy A, Nemeth P, Molnar J. An alternative purification protocol for producing hepatitis B virus X antigen on a preparative scale in Escherichia coli. J Biotechnol. 1997 Aug 11;56(2):81-8. PMID: 9304871 I hope this information is helpful. Should you have any further questions, please do not hesiate to contact us again.

      Read More

      Abcam Scientific Support

      Answered on Sep 26 2007

      Question

      Dear Tracy, May you tell me which amino acid sequence ab2741/Hepatitis B X Protein clone:X36C detect? Vanisa

      Read More

      Abcam community

      Verified customer

      Asked on Nov 05 2003

      Answer

      The immunogen used was the whole recombinant Hepatitis B X protein and the epitope has not been determined.

      Read More

      Abcam Scientific Support

      Answered on Nov 05 2003

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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