Anti-Hepatitis B Virus X antigen 抗体 [X36C] (ab2741)
Key features and details
- Mouse monoclonal [X36C] to Hepatitis B Virus X antigen
- Suitable for: IHC-P, ELISA, IP, WB
- Reacts with: Hepatitis B virus
- Isotype: IgG1
製品の概要
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製品名
Anti-Hepatitis B Virus X antigen antibody [X36C]
Hepatitis B Virus X antigen 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [X36C] to Hepatitis B Virus X antigen -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, ELISA, IP, WBmore details -
種交差性
交差種: Hepatitis B virus -
免疫原
Recombinant full length protein corresponding to Hepatitis B Virus X antigen.
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特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
HPTLC -
一次抗体 備考
Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein-protein interaction. -
ポリ/モノ
モノクローナル -
クローン名
X36C -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab2741 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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IHC-P | Use at an assay dependent concentration. | |
ELISA | Use at an assay dependent concentration. | |
IP | Use at an assay dependent concentration. | |
WB | Use a concentration of 1 µg/ml. |
ターゲット情報
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関連性
Hepatitis B virus X protein (HBx) is a 17 kD transcriptional coactivator that plays a significant role in the regulation of genes involved in inflammation and cell survival. It regulates many transcription factors including nuclear factor kappa B (NF-kappaB) and plays a key role in hepatocarcinogenesis. HBx facilitates the binding of cAMP response element binding protein (CREB) to its responsive element. HBx stabilizes the cellular coactivator ASC-2 through direct protein-protein interaction, affecting the regulation of genes actively transcribed in liver cancer cells. HBx transactivates both JNK and MAPK signal transduction pathways in association with the mobilization of cytosolic Ca2+. The communication between HBx and general transcription factor TFIIB is also one of the mechanisms which account for its transcriptional transactivation. HBx decreased the expression of PTEN a known tumor suppressor and a negative regulator of phosphatidylinositol 3'-kinase/AKT and HBx decreased the expression of PTEN in HBx-transfected cells. The etiology of hepatocellular carcinoma (HCC) is involved with hepatitis B virus (HBV) infection and HBx in particular plays a role in the development of HBV-related HCC. The persistence of HBx is important to the pathogenesis of early HCC and HBx expression in the liver during chronic HBV infection may be an important prognostic marker for the development of HCC. -
参照データベース
- SwissProt: O91531 Hepatitis B virus
- SwissProt: Q4R1S1 Hepatitis B virus
- SwissProt: Q91C38 Hepatitis B virus
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別名
- HBV X antigen antibody
- HBx antibody
- HBxAg antibody
画像
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Western blot - Anti-Hepatitis B Virus X antigen antibody [X36C] (ab2741)Image from Yip WK et al., PLoS One. 2011;6(8):e22888. Epub 2011 Aug 4. Fig 2.; doi:10.1371/journal.pone.0022888; August 4, 2011, PLoS ONE 6(8): e22888.All lanes : Anti-Hepatitis B Virus X antigen antibody [X36C] (ab2741) at 1/2000 dilution
Lane 1 : MIHA hepatocytes
Lane 2 : MIHA hepatocytes infected with lentivirus containing EGFP alone
Lane 3 : MIHA hepatocytes infected with lentivirus containing full length Hepatitis B Virus X antigen
Lanes 4-5 : MIHA hepatocytes infected with lentivirus containing truncated Hepatitis B Virus X antigen
プロトコール
データシートおよび資料
参考文献 (7)
ab2741 は 7 報の論文で使用されています。
- You DG et al. Hepatitis B virus X protein induces size-selective membrane permeabilization through interaction with cardiolipin. Biochim Biophys Acta Biomembr 1861:729-737 (2019). PubMed: 30658058
- Zha Y et al. Hepatitis B virus X protein promotes epithelial-mesenchymal transition and metastasis in hepatocellular carcinoma cell line HCCLM3 by targeting HMGA2. Oncol Lett 16:5709-5714 (2018). PubMed: 30356986
- Lee HR et al. A direct role for hepatitis B virus X protein in inducing mitochondrial membrane permeabilization. J Viral Hepat 25:412-420 (2018). PubMed: 29193612
- Wang B et al. Hepatitis B virus infection is not associated with fatty liver disease: Evidence from a cohort study and functional analysis. Mol Med Rep N/A:N/A (2018). PubMed: 30387826
- Zhong L et al. Reactive Oxygen Species-Mediated c-Jun NH2-Terminal Kinase Activation Contributes to Hepatitis B Virus X Protein-Induced Autophagy via Regulation of the Beclin-1/Bcl-2 Interaction. J Virol 91:N/A (2017). PubMed: 28515304
- Huang P et al. Hepatitis B Virus X Protein (HBx) Is Responsible for Resistance to Targeted Therapies in Hepatocellular Carcinoma: Ex Vivo Culture Evidence. Clin Cancer Res 21:4420-30 (2015). PubMed: 26059188
- Yip WK et al. Carboxyl-terminal truncated HBx regulates a distinct microRNA transcription program in hepatocellular carcinoma development. PLoS One 6:e22888 (2011). WB . PubMed: 21829663