Anti-Hepatitis B Virus Surface Antigen 抗体 [3E7], prediluted (ab859)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis B Virus Surface Antigen antibody [3E7], prediluted (ab859)

    Key features and details

    • Mouse monoclonal [3E7] to Hepatitis B Virus Surface Antigen, prediluted
    • Suitable for: IHC-P
    • Reacts with: Hepatitis B virus
    • Isotype: IgG1

    製品の概要

    • 製品名

      Anti-Hepatitis B Virus Surface Antigen antibody [3E7], prediluted
      Hepatitis B Virus Surface Antigen 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [3E7] to Hepatitis B Virus Surface Antigen, prediluted

    • 由来種

      Mouse

    • 特異性

      This antibody stains the cytoplasm of antigen-positive liver cells in patients with Type B viral hepatitis. This antibody reacts with the ‘a’ determinant present on the HBsAg subtypes ayw1, ayw2, ayw3, ayw4, ayr, adw2, adw4 and adr. It does not react with normal tissues.

    • アプリケーション

      適用あり: IHC-Pmore details

    • 種交差性

      交差種: Hepatitis B virus

    • 免疫原

      Full length protein corresponding to Mouse Hepatitis B Virus Surface Antigen. BALB/c mice were immunized with purified HBsAG subtype AYW3.

    • ポジティブ･コントロール

      • IHC-P: Hepatitis B infected liver tissue.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab859の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P
    1/25 - 1/50.

    Pretreat tissue with citrate buffer pH 6. Incubate with primary antibody for 30 minutes at room temperature.
    特記事項
    IHC-P
    1/25 - 1/50.

    Pretreat tissue with citrate buffer pH 6. Incubate with primary antibody for 30 minutes at room temperature.

    ターゲット情報

    • 関連性

      Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction.

    • 細胞内局在

      Virion membrane

    • 参照データベース

      • 別名

        • Large envelope protein antibody
        • Large surface protein antibody
        • LHB antibody
        • Major surface antigen antibody

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis B Virus Surface Antigen antibody [3E7], prediluted (ab859)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis B Virus Surface Antigen antibody [3E7], prediluted (ab859)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Hepatitis B infected liver tissue labeling Hepatitis B Virus Surface Antigen with ab859.

      参考文献 (1)

      ab859 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab859 は 1 報の論文で使用されています。

      • He Z  et al. Expression of hepatitis B surface antigen in liver tissues can serve as a predictor of prognosis for hepatitis B virus-related hepatocellular carcinoma patients after liver resection. Eur J Gastroenterol Hepatol 33:76-82 (2021). PubMed: 32049678

      レビューと Q&A

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      1-3 of 3 Abreviews or Q&A

      Question

      Hi Abcam Tech Support Team,

      May I know which Human HBsAg Monoclonal antibody (i.e. ab110600, ab54247 and ab20724) binds to all four serotypes of Hepatitis B virus?

      I look forward to receiving your reply soon. Many thanks in advance!

      Best regards,

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      Answer

      Thank you for your email.
      We do offer a variety of antibodies that will recognize the four major serotypes: adr, adw, ayr, ayw.
      If it has been tested which serotype is recognizwed by an antibody this information can be found on the datasheet in the "specificity" section.
      Here is a non- exhaustive list of antibodies that bind all four major serotypes. They have even been characterized further and the datasheet will tell you the serotype subtypes (e.g. ayw2, ayw3, ayw4, ayr, adw2, adw4, adrq+, adrq-)also.
      For example:
      ab2039
      https://www.abcam.com/Hepatitis-B-Virus-Surface-Antigen-antibody-HB12-ab2039.html (or use the following: https://www.abcam.com/Hepatitis-B-Virus-Surface-Antigen-antibody-HB12-ab2039.html).
      ab8333
      https://www.abcam.com/Hepatitis-B-Virus-Surface-Antigen-antibody-HB5-ab8333.html (or use the following: https://www.abcam.com/Hepatitis-B-Virus-Surface-Antigen-antibody-HB5-ab8333.html).
      ab859
      https://www.abcam.com/Hepatitis-B-Virus-Surface-Antigen-antibody-3E7-prediluted-ab859.html (or use the following: https://www.abcam.com/Hepatitis-B-Virus-Surface-Antigen-antibody-3E7-prediluted-ab859.html).
      ab20724
      https://www.abcam.com/Hepatitis-B-Virus-Surface-Antigen-Ayw-antibody-HB5-ab20724.html (or use the following: https://www.abcam.com/Hepatitis-B-Virus-Surface-Antigen-Ayw-antibody-HB5-ab20724.html).
      Which antibody is the most suitable for experiments depends on your samples and the planned application.
      We encourage customers to consult the latest literature available through PubMed and other resources in order to find the most up-to-date information about this specific research topic.
      I hope this information is helpful. Please do not hesiate to contact me again with anyfurther questions.

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      Question

      ab859 Hepatitis B Virus Surface Antigen antibody [3E7], prediluted Can you provide the concentration information?

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      Answer

      Regarding ab859 (Hepatitis B Virus Surface Antigen antibody [3E7], prediluted), the concentration of the present lot is 2 ug/ml. Please don't hesitate to contact us again if you have further questions.

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      Question

      Hello, Just had a quick question about product ab859. This is a pre-diluted antibody - What is the final concentration? Thanks, Mike

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      Answer

      The final concentration of this prediluted antibody is 20ug/ml.

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