Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) 抗体 (ab9193)

Reviews (1)Q&A (7)References (14)

Key features and details

  • Horse polyclonal to Hepatitis B Virus Surface Antigen (Ad/Ay)
  • Suitable for: ELISA
  • Reacts with: Hepatitis B virus
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody
    Hepatitis B Virus Surface Antigen (Ad/Ay) 一次抗体 製品一覧

  • 製品の詳細

    Horse polyclonal to Hepatitis B Virus Surface Antigen (Ad/Ay)

  • 由来種

    Horse

  • 特異性

    This antibody is specific for Hepatitis B surface antigen

  • アプリケーション

    適用あり: ELISAmore details

  • 種交差性

    交差種: Hepatitis B virus

  • 免疫原

    Immunization of horses with highly purified Ad/Ay antigen.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab9193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.

  • 細胞内局在

    Virion membrane

  • 参照データベース

    see all

  • 別名

    • HBsAg antibody
    • HBV major surface antigen antibody
    • HBV surface antigen antibody
    • Hepatitis B Virus major surface antigen antibody
    • Major surface antigen antibody
    • S antibody
    see all

参考文献 (14)

ab9193 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab9193 は 14 報の論文で使用されています。

  • Huang LH  et al. Development of a Transgenic Flammulina velutipes Oral Vaccine for Hepatitis B. Food Technol Biotechnol 57:105-112 (2019). PubMed: 31316282
  • Gani AW  et al. An Automated, Quantitative, and Multiplexed Assay Suitable for Point-of-Care Hepatitis B Virus Diagnostics. Sci Rep 9:15615 (2019). PubMed: 31666635
  • Passioura T  et al. De Novo Macrocyclic Peptide Inhibitors of Hepatitis B Virus Cellular Entry. Cell Chem Biol 25:906-915.e5 (2018). PubMed: 29779957
  • Song J  et al. CRISPR/Cas9-mediated knockout of HBsAg inhibits proliferation and tumorigenicity of HBV-positive hepatocellular carcinoma cells. J Cell Biochem N/A:N/A (2018). PubMed: 29904948
  • Kim DH  et al. Intracellular interleukin-32? mediates antiviral activity of cytokines against hepatitis B virus. Nat Commun 9:3284 (2018). PubMed: 30115930
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-8 of 8 Abreviews or Q&A

Question

What does the AD/AY mean in the Hepatitis B surface antigen antibody

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Answer



The designation Ad and Ay refer to the subtypes of Hepatitis B Surface Antigen. The reference in the product profile simply means that the antigen used was a mixture of both rather than one or the other.

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Question

Our records indicate that Cedarlane has only received lot# GR62259 of AB9193.
Please confirm that this lot is not affected by this notification.
Thank you

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Answer



Unfortunately, I have to be the messenger to confirm that indeed this lot is affected.

Please let me know what kind of compensation your customer would like to receive from us.

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Question

I tried standard protocol (bicarb pH9.6 coating, blocking 1% BSA, then sandwich with your sAg standard, and detection with 2nd goat anti mouse HRP).

I ordered this Ab pair you suggested. Can you pls give me the protocol details that have worked for Abcam? I'll be using the same sAg standard that I ordered before.

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Answer

Here are a few specific sELISA protocol details I was able to find:

ab2039, the capture antibody, was coated on the plate at 5 μg/ml in 0.1 M carbonate buffer, and the HRP-conjugated detection antibody, ab2042, was used at 0.5-5 ug/mL.

Otherwise, a standard sELISA protocol such as https://www.abcam.com/index.html?pageconfig=resource&rid=11422 (or your current in-house protocol) can be used with appropriate negative controls. One thing I could not find was what source for Hepatitis B Virus Surface Antigen was used in this sELISA.

I hope this is helpful. Please contact me again if you have any further questions.

Use our products? Submit an https://www.abcam.com/abreviews. Earn rewards!

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Question

Inquiry: I purchased two anti HBsAg antibodies (ab9193 and ab8636) and ab113610 protein as standard but don't see any signal in my ELISA attempt. Which antibody pairs have you tested for ELISA? Can you recommend a detection Ab that I can use with ab9193, e.g. HRP conjugated ab19990 or ab2042?

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Answer



If you don't mind providing a more complete protocol for the ELISA assay you ran, I can help you to troubleshoot further.

We have already tested ab2039 to work successfully with ab2042 in sandwich ELISA.

We have not yet determined which detection antibodies may work with ab9193 in and ELISA.

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Question

Hi there, Do you know the epitope that is recognised by this antibody? Also do you have any further information on the antigen used to raise this antibody? Many thanks

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Answer

Thank you for contacting us.
The antigen was purified from human serum, heat inactivated and injected into horse. The exact immunogen sequence is not known; immunogen contains L, M & S proteins.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

How was the concentration of this antibody determined? Have you by any chance also determined the purity of the immunoglobulins?

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Answer

Vielen Dank für Ihre Anfrage. Es tut mir leid, dass Sie so lange auf eine Antwort warten mussten. Meine Kollegin Claudia, mit der Sie telefoniert hatten, ist derzeit für Abcam unterwegs und nicht im Büro, hat zuvor jedoch noch genauere Informationen zu ab9193 erfragt. Die Informationen, die uns nun mitgeteilt wurden, besagen, dass die Konzentration von ab9193 photometrisch bestimmt wurde. Die Reinheit des affinitätsaufgereinigten Antikörpers wird leider nicht routinemäßig bestimmt. Wir versuchen nach Möglichkeit alle wichtigen Daten über unsere Produkte an unsere Kunden weiterzugeben und ich möchte mich dafür entschuldigen, dass dies in diesem Fall nicht möglich ist. Ich hoffe, diese Informationen helfen Ihnen dennoch weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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Immunocytochemistry/ Immunofluorescence abreview for Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody

 Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Huh7 Human Hepatoma Cells)
Specification
Huh7 Human Hepatoma Cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Saponin
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Abcam user community

Verified customer

投稿 Mar 22 2011

Question

One of our customers would like to get the dillution for these antibody for WB. He would also be interestested to get a picture of a WB with these products. Do you think this would be possible? Thank you in advance for your help.

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Answer

I would recommend a starting dilution of 1:1500 with this product. Currently we do not have an image available with this antibody but we will provide you with one when it becomes available.

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