製品の概要

  • 製品名
    Anti-Hepatitis B Virus Core Antigen antibody [14E11]
    Hepatitis B Virus Core Antigen 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [14E11] to Hepatitis B Virus Core Antigen
  • 由来種
    Mouse
  • アプリケーション
    適用あり: ELISA, IHC-FoFr, IHC-Fr, ICC/IF, IP, IHC-P, WBmore details
  • 種交差性
    交差種: Hepatitis B virus
  • 免疫原

    Purified Denatured Hepatitis B Core Antigen

  • エピトープ
    135-141aa (PNAPILS)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab8638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
IHC-FoFr 1/100.
IHC-Fr 1/100.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P 1/100.
WB 1/1000.

ターゲット情報

  • 関連性
    Hepatitis B Virus Core Antigen (HBcAg) is part of the infectious virion containing an inner "core particle" enclosing the viral genome. The icosahedral core particle contains 180 or 240 copies of the core protein. HBcAg is one of the three major clinical antigens of hepatitis B virus but disappears early in the course of infection. The hepatitis B virus core antigen (HBcAg) is a highly immunogenic subviral particle and functions as both a T-cell-dependent and a T-cell-independent antigen. Therefore, HBcAg may be a promising candidate target for therapeutic vaccine control of chronic HBV infection.
  • 細胞内局在
    Capsid protein: Virion. Host cytoplasm, hepatocyte nucleus.
  • 参照データベース
    • 別名
      • C antibody
      • Capsid protein antibody
      • Core and e antigen antibody
      • core antibody
      • Core antigen antibody
      • Core protein antibody
      • HBc antibody
      • HBcAg antibody
      • HBVgp4 antibody
      • Hepatitis B core antigen antibody
      • Hepatitis B Virus core antigen antibody
      • p21.5 antibody
      • precore/core protein antibody
      see all

    参考文献

    This product has been referenced in:
    • Liang P  et al. Efficient Humoral and Cellular Immune Responses Induced by a Chimeric Virus-like Particle Displaying the Epitope of EV71 without Adjuvant. Biomed Environ Sci 31:343-350 (2018). WB ; Hepatitis B virus . Read more (PubMed: 29866216) »
    • Ye X  et al. Chimeric virus-like particle vaccines displaying conserved enterovirus 71 epitopes elicit protective neutralizing antibodies in mice through divergent mechanisms. J Virol 88:72-81 (2014). Read more (PubMed: 24131712) »
    See all 5 Publications for this product

    レビューと Q&A

    1-10 of 12 Abreviews or Q&A

    Answer

    Thank for your inquiry. I would like to reassure you that the antibody is the same as previously. There is no batch to batch variability of the immunogen sequence in monoclonal antibodies. This a monoclonal antibody was known to bind to the HBV Core Antigen which corresponds to the area of amino acid residues 130-140. More recently we discovered that the epitope had been mapped and the literature indicates that this clone (14E11) recognizes the amino acids 135-141 which corresponds to the following sequence: PNAPILS. I hope this information is helpful. Please do not hesitate to contact me again with any further questions.

    Read More

    Answer

    1. The epitope is 135-141 (PNAPILS) 2. This depends on how much virus or core antigen is in the cells. You can load different amounts in parallel. For example 5ug (microgram) and 20ug. 3. Example version of Laemmli 2X buffer 4% SDS 10% 2-mercaptoehtanol 20% glycerol 0.004% bromophenol blue 0.125 M Tris HCl Check the pH and bring it to pH 6.8. Our online protocols are at www.abcam.com/protocols I highly recommend to look at the publications: PubMed: 19327810 and 16378961.

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    Answer

    Thank you for your enquiry. Further to discussions with the lab I have determined that the approach that you are using (n)on-reducing gel at 12%) is not the best system to isolate monomeric units of HBcAg core particles. To dissolve HBcAg core particles to monomers, the sample should be dissolved in the Laemmli buffer with 5%SDS, 5% Mercaptoethanol (added fresh each time), and boiled for 10 min on a water bath. Should you continue to get what you expect to be polymers of this protein please do not hesitate to get back in touch with me.

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    Answer

    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I am surprised by the size of the band that you have been detecting. I am in correspondence with the source of this antibody to determine the expected size of the band using this antibody. At this stage I would like to recommend that you transfer the protein lysate that you have prepared and cut the blot using a scalpel so that only proteins of a molecular weight less than 100KDa are immunoblotted and the antibody is concentrated over proteins of appropriate size. I would like to recommend that an overnight incubation is performed using 3% BSA as a blocking agent. This approach should significantly increase the chances of detecting the Hepatitis core antigen. I hope this information helps. Should this approach not improve your results please do not hesitate to get back in touch with me.

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    Answer

    Ab8637 and ab8639 should recognize the precorerotein in WB but there is no "cross-reaction" as all proteins are distinguishable by mobility on the gel (i.e they will recognise both the precoreprotein and core protein). AB8638 may or may not recognize your protein fragment aa1-150, since its epitope is aa aa35-140 of the core protein, which is really close to the N terminus of the protein you are going to analyze. Ab8637 will not recognize the precorprotein under native conditions, because this protein can not self assemble into particles. I hope this information will help you, please let me know if you need further assistance,

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    Answer

    Here is the information that I could obtain regarding ab8639, ab8638, and ab8637. The recommended starting dilutions are 1:1,000 and down in 10-fold increments. As for detection, any known detection method would work. Once again, those mabs had being characterized in great detail in the following paper: Bichko et al. Epitopes recognized by antibodies to denatured core protein of hepatitis B virus. Mol Immunol. 1993 Feb;30(3):221-31 If you have any more questions, just let me know.

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    Answer

    I have received the following information regarding ab8255 and ab2045, but will have to get back to you about the other antibodies. The originator has said that they tested the antibodies as pairs in internal ELISA. Possible pairs are H6F5 (ab2045) - H3A4 (ab8255), and H3A4 - H6F5. They have used coating concentration 5 - 10 ug/ml and conjugate concentration 0.2 -1 ug/ml. However, the concentrations need to be optimized separately for every application.

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    Answer

    Thank you for your enquiry. I was able to obtain the following information regarding the use of these antibodies in ELISA. Ab8255 and Ab2045: These antibodies have been tested in ELISA with the native antigen. The antibodies can be used as a pair in sandwich ELISA. Both clones can work as capture and as detection antibodies. Ab8638, Ab8637, and Ab8639: These antibodies work well with a recombinant or natural HBcAg adsorbed on a plate, or in sandwich ELISA. Also, they work well with the synthetic HBcAg peptides. Below is one reference: Bichko et al. Epitopes recognized by antibodies to denatured core protein of hepatitis B virus. Mol Immunol. 1993 Feb;30(3):221-31

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    Answer

    Thank you for your reply. The only data we have regarding this is that it is an ayw variant as described in: Bichko et. al. Subtype ayw variant of hepatitis B virus. DNA primary structure analysis. FEBS Lett. 1985 Jun 3;185(1):208-12.

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    1-10 of 12 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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