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Anti-Heparan Sulfate Proteoglycan 2/Perlecan 抗体 [EPR22844-82] - BSA and Azide free (ab269589)

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Western blot - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [EPR22844-82P] - BSA and Azide free (ab269589)
  • Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [EPR22844-82] - BSA and Azide free (ab269589)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22844-82] to Heparan Sulfate Proteoglycan 2/Perlecan - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [EPR22844-82] - BSA and Azide free
    Heparan Sulfate Proteoglycan 2/Perlecan 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR22844-82] to Heparan Sulfate Proteoglycan 2/Perlecan - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HUVEC and SH-SY5Y whole cell lysates; human heart tissue lysate.

  • 特記事項

    ab269589 is the carrier-free version of ab255829. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab269589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
WB
Human
アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 469, 130 kDa (predicted molecular weight: 469 kDa).
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 469, 130 kDa (predicted molecular weight: 469 kDa).
  • 追加情報
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

    • ターゲット情報

    • 機能

      Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development.
      Endorepellin in an anti-angiogenic and anti-tumor peptide that inhibits endothelial cell migration, collagen-induced endothelial tube morphogenesis and blood vessel growth in the chorioallantoic membrane. Blocks endothelial cell adhesion to fibronectin and type I collagen. Anti-tumor agent in neovascularization. Interaction with its ligand, integrin alpha2/beta1, is required for the anti-angiogenic properties. Evokes a reduction in phosphorylation of receptor tyrosine kinases via alpha2/beta1 integrin-mediated activation of the tyrosine phosphatase, PTPN6.
      The LG3 peptide has anti-angiogenic properties that require binding of calcium ions for full activity.

    • 組織特異性

      Found in the basement membranes.

    • 関連疾患

      Defects in HSPG2 are the cause of Schwartz-Jampel syndrome (SJS1) [MIM:255800]; a rare autosomal recessive disorder characterized by permanent myotonia (prolonged failure of muscle relaxation) and skeletal dysplasia, resulting in reduced stature, kyphoscoliosis, bowing of the diaphyses and irregular epiphyses.
      Defects in HSPG2 are the cause of dyssegmental dysplasia Silverman-Handmaker type (DDSH) [MIM:224410]. The dyssegmental dysplasias are rare, autosomal recessive skeletal dysplasias with anisospondyly and micromelia. There are two recognized types: the severe, lethal DDSH and the milder Rolland-Desbuquois form. Individuals with DDSH also have a flat face, micrognathia, cleft palate and reduced joint mobility, and frequently have an encephalocoele. The endochondral growth plate is short, the calcospherites (which are spherical calcium-phosphorus crystals produced by hypertrophic chondrocytes) are unfused, and there is mucoid degeneration of the resting cartilage.

    • 配列類似性

      Contains 4 EGF-like domains.
      Contains 22 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 11 laminin EGF-like domains.
      Contains 3 laminin G-like domains.
      Contains 3 laminin IV type A domains.
      Contains 4 LDL-receptor class A domains.
      Contains 1 SEA domain.

    • 翻訳後修飾

      Proteolytic processing produces the C-terminal angiogenic peptide, endorepellin. This peptide can be further processed to produce the LG3 peptide.
      N- and O-glycosylated; contains three heparan sulfate chains. The LG3 peptide contains at least three and up to five potential O-glycosylation sites but no N-glycosylation.

    • 細胞内局在

      Secreted > extracellular space > extracellular matrix > basement membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Basement membrane specific heparan sulfate proteoglycan core protein antibody
        • Endorepellin (domain V region) antibody
        • Heparan sulfate proteoglycan of basement membrane antibody
        • HSPG 2 antibody
        • HSPG antibody
        • Hspg2 antibody
        • LG3 peptide antibody
        • Perlecan antibody
        • PGBM_HUMAN antibody
        • PLC antibody
        • Schwartz Jampel syndrome 1 (chondrodystrophic myotonia) antibody
        • SJA antibody
        • SJS antibody
        • SJS1 antibody
        see all

      画像

      • Western blot - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [EPR22844-82P] - BSA and Azide free (ab269589)
        Western blot - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [EPR22844-82P] - BSA and Azide free (ab269589)
        All lanes : Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [EPR22844-82] (ab255829) at 1/1000 dilution

        Lane 1 : HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg
        Lane 2 : HUVEC culture supernatant at 10 µl
        Lane 3 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg
        Lane 4 : SH-SY5Y culture supernatant at 10 µl
        Lane 5 : Human heart tissue lysate at 20 µg

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

        Predicted band size: 469 kDa
        Observed band size: 130,469 kDa why is the actual band size different from the predicted?



        Blocking and dilution buffer: 5% NFDM/TBST.

        Exposure times.

        Lane 1:8 seconds; Lanes 2-5: 3 minutes.

        The molecular weight observed is consistent with what has been described in the literature (PMID: 14627628, 12591930,23235151, 26636652).

        Lower expression on SH-SY5Y (PMID:25883214).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255829).

      • Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [EPR22844-82] - BSA and Azide free (ab269589)
        Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [EPR22844-82] - BSA and Azide free (ab269589)

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (0)

      ab269589 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab269589 は論文での使用が確認できていません。

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