Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human H9 Organoids)
Permeabilization
Yes - 0.05% Tween 20
Specification
Human H9 Organoids
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Sea Block
Dilution
1/100
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary A
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
The Heparan Sulfate Proteoglycan 2/Perlecan antibody is pseudo colored in Green and is expressed in the kidney organoid progenitor tubules and not in the podocalyxin positive progenitor cells. The nuclei are stained Blue with Hoechst .
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投稿 Feb 06 2020