Anti-Heparan Sulfate Proteoglycan 2/Perlecan 抗体 [A7L6] (ab2501)
Key features and details
- Rat monoclonal [A7L6] to Heparan Sulfate Proteoglycan 2/Perlecan
- Suitable for: ICC, IHC-P, IHC-Fr, IP, WB
- Reacts with: Mouse, Rat, Cow, Human, Pig, Fish
- Isotype: IgG2a
製品の概要
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製品名
Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6]
Heparan Sulfate Proteoglycan 2/Perlecan 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [A7L6] to Heparan Sulfate Proteoglycan 2/Perlecan -
由来種
Rat -
特異性
Recognizes domain IV of heparan sulphate proteoglycan 2 / perlecan. The reactivity is independent of the galactosaminoglycan moieties. Therefore, the epitope is not sensitive to heparitinase treatment. -
アプリケーション
適用あり: ICC, IHC-P, IHC-Fr, IP, WBmore details -
種交差性
交差種: Mouse, Rat, Cow, Human, Pig, Fish -
免疫原
Tissue, cells or virus corresponding to Heparan Sulfate Proteoglycan 2/Perlecan. High molecular mass material derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG.
Database link: P98160 -
特記事項
Proteoglycans are macromolecules consisting of a variety of core proteins with covalently attached one or several polysaccharide chains of the glycosaminoglycan type (heparan sulphate, heparin, chondroitin sulphate, dermatan sulphate or keratan sulphate). At least two forms of basement membrane heparan sulphate proteoglycan (HSPG) have been identified. One with a large core protein (> 400 kD) and one with a small core protein (30 kD). The large HSPG is probably the most abundant basement membrane proteoglycan. It is located predominantly in the lamina lucida, where it forms clustered aggregates and interacts with other basement membrane components to form the matrix. In addition, it also plays a critical role in attachment of cells to the basal membrane via integrin receptors.
This product was previously labelled as Heparan Sulfate Proteoglycan 2
Source: A7L6 is a Rat monoclonal IgG2a antibody derived by fusion of X63 Ag8.653 Mouse myeloma cells with spleen cells from a Fisher Rat immunized with high molecular mass material derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG.
Formulation: The vial contains 100 ul 1 mg/ml monoclonal purified antibody in PBS containing 0.09% sodium azide.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
精製度
Protein A purified -
一次抗体 備考
Proteoglycans are macromolecules consisting of a variety of core proteins with covalently attached one or several polysaccharide chains of the glycosaminoglycan type (heparan sulphate, heparin, chondroitin sulphate, dermatan sulphate or keratan sulphate). At least two forms of basement membrane heparan sulphate proteoglycan (HSPG) have been identified. One with a large core protein (> 400 kD) and one with a small core protein (30 kD). The large HSPG is probably the most abundant basement membrane proteoglycan. It is located predominantly in the lamina lucida, where it forms clustered aggregates and interacts with other basement membrane components to form the matrix. In addition, it also plays a critical role in attachment of cells to the basal membrane via integrin receptors. -
ポリ/モノ
モノクローナル -
クローン名
A7L6 -
ミエローマ
x63-Ag8.653 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab2501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC | 1/25 - 1/200. Use amplification with ABC (avidin biotin complex). | |
| IHC-P | Use at an assay dependent dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. Use amplification with ABC (avidin biotin complex). | |
| IHC-Fr | Use at an assay dependent dilution. PubMed: 16055495Use amplification with ABC (avidin biotin complex). | |
| IP | Use at an assay dependent dilution. | |
| WB | 1/100 - 1/1000. |
ターゲット情報
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機能
Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development.
Endorepellin in an anti-angiogenic and anti-tumor peptide that inhibits endothelial cell migration, collagen-induced endothelial tube morphogenesis and blood vessel growth in the chorioallantoic membrane. Blocks endothelial cell adhesion to fibronectin and type I collagen. Anti-tumor agent in neovascularization. Interaction with its ligand, integrin alpha2/beta1, is required for the anti-angiogenic properties. Evokes a reduction in phosphorylation of receptor tyrosine kinases via alpha2/beta1 integrin-mediated activation of the tyrosine phosphatase, PTPN6.
The LG3 peptide has anti-angiogenic properties that require binding of calcium ions for full activity. -
組織特異性
Found in the basement membranes. -
関連疾患
Defects in HSPG2 are the cause of Schwartz-Jampel syndrome (SJS1) [MIM:255800]; a rare autosomal recessive disorder characterized by permanent myotonia (prolonged failure of muscle relaxation) and skeletal dysplasia, resulting in reduced stature, kyphoscoliosis, bowing of the diaphyses and irregular epiphyses.
Defects in HSPG2 are the cause of dyssegmental dysplasia Silverman-Handmaker type (DDSH) [MIM:224410]. The dyssegmental dysplasias are rare, autosomal recessive skeletal dysplasias with anisospondyly and micromelia. There are two recognized types: the severe, lethal DDSH and the milder Rolland-Desbuquois form. Individuals with DDSH also have a flat face, micrognathia, cleft palate and reduced joint mobility, and frequently have an encephalocoele. The endochondral growth plate is short, the calcospherites (which are spherical calcium-phosphorus crystals produced by hypertrophic chondrocytes) are unfused, and there is mucoid degeneration of the resting cartilage. -
配列類似性
Contains 4 EGF-like domains.
Contains 22 Ig-like C2-type (immunoglobulin-like) domains.
Contains 11 laminin EGF-like domains.
Contains 3 laminin G-like domains.
Contains 3 laminin IV type A domains.
Contains 4 LDL-receptor class A domains.
Contains 1 SEA domain. -
翻訳後修飾
Proteolytic processing produces the C-terminal angiogenic peptide, endorepellin. This peptide can be further processed to produce the LG3 peptide.
N- and O-glycosylated; contains three heparan sulfate chains. The LG3 peptide contains at least three and up to five potential O-glycosylation sites but no N-glycosylation. -
細胞内局在
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 444872 Cow
- Entrez Gene: 3339 Human
- Entrez Gene: 15530 Mouse
- Entrez Gene: 313641 Rat
- Omim: 142461 Human
- SwissProt: P98160 Human
- SwissProt: Q05793 Mouse
- Unigene: 562227 Human
see all -
別名
- Basement membrane specific heparan sulfate proteoglycan core protein antibody
- Endorepellin (domain V region) antibody
- Heparan sulfate proteoglycan of basement membrane antibody
see all
画像
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![Immunohistochemistry (Frozen sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (ab2501)](https://www.abcam.co.jp/ps/products/2/ab2501/Images/ab2501-280731-anti-heparan-sulfate-proteoglycan-2-antibody-a7l6-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (ab2501)IHC image on a frozen section of human colon showing strong reactivity in the extracellular matrix and basement membrane.
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![Immunohistochemistry (Frozen sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (ab2501)](https://www.abcam.co.jp/ps/products/2/ab2501/Images/ab2501-5878-anti-heparan-sulfate-proteoglycan-2-antibody-a7l6-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A7L6] (ab2501)Immunohistochemistry on frozen section of Human kidney showing strong reactivity in the extracellular matrix and basement membrane.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献
ab2501 は 19 報の論文で使用されています。
- Horsley H et al. A urine-dependent human urothelial organoid offers a potential alternative to rodent models of infection. Sci Rep 8:1238 (2018). PubMed: 29352171
- Brauchle E et al. Biomechanical and biomolecular characterization of extracellular matrix structures in human colon carcinomas. Matrix Biol 68-69:180-193 (2018). IHC ; Human . PubMed: 29605717
- Sambi M et al. Acellular Mouse Kidney ECM can be Used as a Three-Dimensional Substrate to Test the Differentiation Potential of Embryonic Stem Cell Derived Renal Progenitors. Stem Cell Rev 13:513-531 (2017). PubMed: 28239758
- Shu C et al. Use of FGF-2 and FGF-18 to direct bone marrow stromal stem cells to chondrogenic and osteogenic lineages. Future Sci OA 2:FSO142 (2016). PubMed: 28116125
- Chen AC et al. Physical and functional interaction between the a- and ?-secretases: A new model of regulated intramembrane proteolysis. J Cell Biol 211:1157-76 (2015). PubMed: 26694839
